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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ALDH9A1 ALDH4, ALDH7, ALDH9
Sequence Length (AA) Molecular Weight (Da)
494 53802
Protein Name
4-trimethylaminobutyraldehyde dehydrogenase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSTGTFVVSQ PLNYRGGARV EPADASGTEK AFEPATGRVI ATFTCSGEKE
60 70 80 90 100
VNLAVQNAKA AFKIWSQKSG MERCRILLEA ARIIREREDE IATMECINNG
110 120 130 140 150
KSIFEARLDI DISWQCLEYY AGLAASMAGE HIQLPGGSFG YTRREPLGVC
160 170 180 190 200
VGIGAWNYPF QIASWKSAPA LACGNAMVFK PSPFTPVSAL LLAEIYSEAG
210 220 230 240 250
VPPGLFNVVQ GGAATGQFLC QHPDVAKVSF TGSVPTGMKI MEMSAKGIKP
260 270 280 290 300
VTLELGGKSP LIIFSDCDMN NAVKGALMAN FLTQGQVCCN GTRVFVQKEI
310 320 330 340 350
LDKFTEEVVK QTQRIKIGDP LLEDTRMGPL INRPHLERVL GFVKVAKEQG
360 370 380 390 400
AKVLCGGDIY VPEDPKLKDG YYMRPCVLTN CRDDMTCVKE EIFGPVMSIL
410 420 430 440 450
SFDTEAEVLE RANDTTFGLA AGVFTRDIQR AHRVVAELQA GTCFINNYNV
460 470 480 490 494
SPVELPFGGY KKSGFGRENG RVTIEYYSQL KTVCVEMGDV ESAF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-309 Collapse assay details

Data source: Panorama

Official Gene Symbol
ALDH9A1
Peptide Sequence
IGDPLLEDTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
317
Peptide End
326
CPTAC ID
CPTAC-309
Peptide Molecular Mass
1,127.5823
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 25.1 18.1 12 28.1 36.8 25 37.7 41 27.7 15 25 15
y6 (1+) 21.2 9.4 5.6 25.6 11.6 15.1 33.2 14.9 16.1 15 25 15
y5 (1+) 10.8 7 5.7 16.1 8.3 5.5 19.4 10.9 7.9 15 25 15
sum 11.3 7.1 5.3 15.5 10 8.6 19.2 12.3 10.1 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-310 Collapse assay details

Data source: Panorama

Official Gene Symbol
ALDH9A1
Peptide Modified Sequence
VLC[+57.0]GGDIYVPEDPK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
355
Peptide - Site of Modification
3
Peptide Start
353
Peptide End
366
CPTAC ID
CPTAC-310
Peptide Molecular Mass
1,560.7494
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 12.1 7 7.3 20 9.2 8.9 23.4 11.6 11.5 15 25 15
y8 (1+) 19.1 9.6 11 21.5 15.1 14.4 28.8 17.9 18.1 15 25 15
y7 (1+) 8.8 8.6 7 13.1 15.3 7.5 15.8 17.6 10.3 15 25 15
sum 7.5 6.1 4.7 13.6 12.1 5.8 15.5 13.6 7.5 15 25 15


Additional Resources and Comments