BASP1, CPTAC-32 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BASP1	NAP22

Sequence Length (AA)	Molecular Weight (Da)
227	22693

Protein Name
Brain acid soluble protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGGKLSKKKK	GYNVNDEKAK	EKDKKAEGAA	TEEEGTPKES	EPQAAAEPAE
60	70	80	90	100
AKEGKEKPDQ	DAEGKAEEKE	GEKDAAAAKE	EAPKAEPEKT	EGAAEAKAEP
110	120	130	140	150
PKAPEQEQAA	PGPAAGGEAP	KAAEAAAAPA	ESAAPAAGEE	PSKEEGEPKK
160	170	180	190	200
TEAPAAPAAQ	ETKSDGAPAS	DSKPGSSEAA	PSSKETPAAT	EAPSSTPKAQ
210	220	227
GPAASAEEPK	PVEAPAANSD	QTVTVKE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-31 Collapse assay details

Data source: Panorama

Official Gene Symbol: BASP1
Peptide Sequence: ESEPQAAAEPAEAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 39
Peptide End: 52

CPTAC ID: CPTAC-31
Peptide Molecular Mass: 1,426.6576
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	2.8	4.3	3.3	5.5	6.7	5	6.2	8	6	15	25	15
y9 (1+)	3.9	3.8	4.8	6.6	6.5	5.8	7.7	7.5	7.5	15	25	15
y8 (1+)	4	4.2	2.5	6.6	6.8	5.3	7.7	8	5.9	15	25	15
sum	3	3.5	2.9	6.1	6.5	4.8	6.8	7.4	5.6	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments