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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CACYBP S100A6BP,SIP
Sequence Length (AA) Molecular Weight (Da)
228 26210
Protein Name
Calcyclin-binding protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASEELQKDL EEVKVLLEKA TRKRVRDALT AEKSKIETEI KNKMQQKSQK
60 70 80 90 100
KAELLDNEKP AAVVAPITTG YTVKISNYGW DQSDKFVKIY ITLTGVHQVP
110 120 130 140 150
TENVQVHFTE RSFDLLVKNL NGKSYSMIVN NLLKPISVEG SSKKVKTDTV
160 170 180 190 200
LILCRKKVEN TRWDYLTQVE KECKEKEKPS YDTETDPSEG LMNVLKKIYE
210 220 228
DGDDDMKRTI NKAWVESREK QAKGDTEF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-321 Collapse assay details

Data source: Panorama

Official Gene Symbol
CACYBP
Peptide Sequence
IYITLTGVHQVPTENVQVHFTER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
89
Peptide End
111
CPTAC ID
CPTAC-321
Peptide Molecular Mass
2,680.3973
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y21 (2+) 22.5 9.6 11.8 23.5 21.9 19.9 32.5 23.9 23.1 25 15 15
y20 (2+) 28.2 18 12.4 33.1 21.1 25 43.5 27.7 27.9 25 15 15
y17 (2+) 34.7 15.5 11.2 42.9 21.1 23.8 55.2 26.2 26.3 25 15 15
sum 18.9 9 7.2 25.2 17.8 20.1 31.5 19.9 21.4 25 15 15

Additional Resources and Comments


Assay Details for CPTAC-322 Collapse assay details

Data source: Panorama

Official Gene Symbol
CACYBP
Peptide Modified Sequence
TDTVLILC[+57.0]R
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
154
Peptide - Site of Modification
8
Peptide Start
147
Peptide End
155
CPTAC ID
CPTAC-322
Peptide Molecular Mass
1,089.5852
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 11.4 15.4 7.9 19.5 21.1 9.1 22.6 26.1 12.1 15 25 15
y5 (1+) 8.8 7.3 4.9 13.6 10.2 6 16.2 12.5 7.7 15 25 15
y4 (1+) 5.8 7.4 7.5 12.6 11.1 7.2 13.9 13.3 10.4 15 25 15
sum 6.8 7.4 5.7 11.9 11.4 6 13.7 13.6 8.3 15 25 15

Additional Resources and Comments