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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ATR

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ATR FRP1
Sequence Length (AA) Molecular Weight (Da)
2644 301367
Protein Name
Serine/threonine-protein kinase ATR
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGEHGLELAS MIPALRELGS ATPEEYNTVV QKPRQILCQF IDRILTDVNV
60 70 80 90 100
VAVELVKKTD SQPTSVMLLD FIQHIMKSSP LMFVNVSGSH EAKGSCIEFS
110 120 130 140 150
NWIITRLLRI AATPSCHLLH KKICEVICSL LFLFKSKSPA IFGVLTKELL
160 170 180 190 200
QLFEDLVYLH RRNVMGHAVE WPVVMSRFLS QLDEHMGYLQ SAPLQLMSMQ
210 220 230 240 250
NLEFIEVTLL MVLTRIIAIV FFRRQELLLW QIGCVLLEYG SPKIKSLAIS
260 270 280 290 300
FLTELFQLGG LPAQPASTFF SSFLELLKHL VEMDTDQLKL YEEPLSKLIK
310 320 330 340 350
TLFPFEAEAY RNIEPVYLNM LLEKLCVMFE DGVLMRLKSD LLKAALCHLL
360 370 380 390 400
QYFLKFVPAG YESALQVRKV YVRNICKALL DVLGIEVDAE YLLGPLYAAL
410 420 430 440 450
KMESMEIIEE IQCQTQQENL SSNSDGISPK RRRLSSSLNP SKRAPKQTEE
460 470 480 490 500
IKHVDMNQKS ILWSALKQKA ESLQISLEYS GLKNPVIEML EGIAVVLQLT
510 520 530 540 550
ALCTVHCSHQ NMNCRTFKDC QHKSKKKPSV VITWMSLDFY TKVLKSCRSL
560 570 580 590 600
LESVQKLDLE ATIDKVVKIY DALIYMQVNS SFEDHILEDL CGMLSLPWIY
610 620 630 640 650
SHSDDGCLKL TTFAANLLTL SCRISDSYSP QAQSRCVFLL TLFPRRIFLE
660 670 680 690 700
WRTAVYNWAL QSSHEVIRAS CVSGFFILLQ QQNSCNRVPK ILIDKVKDDS
710 720 730 740 750
DIVKKEFASI LGQLVCTLHG MFYLTSSLTE PFSEHGHVDL FCRNLKATSQ
760 770 780 790 800
HECSSSQLKA SVCKPFLFLL KKKIPSPVKL AFIDNLHHLC KHLDFREDET
810 820 830 840 850
DVKAVLGTLL NLMEDPDKDV RVAFSGNIKH ILESLDSEDG FIKELFVLRM
860 870 880 890 900
KEAYTHAQIS RNNELKDTLI LTTGDIGRAA KGDLVPFALL HLLHCLLSKS
910 920 930 940 950
ASVSGAAYTE IRALVAAKSV KLQSFFSQYK KPICQFLVES LHSSQMTALP
960 970 980 990 1000
NTPCQNADVR KQDVAHQREM ALNTLSEIAN VFDFPDLNRF LTRTLQVLLP
1010 1020 1030 1040 1050
DLAAKASPAA SALIRTLGKQ LNVNRREILI NNFKYIFSHL VCSCSKDELE
1060 1070 1080 1090 1100
RALHYLKNET EIELGSLLRQ DFQGLHNELL LRIGEHYQQV FNGLSILASF
1110 1120 1130 1140 1150
ASSDDPYQGP RDIISPELMA DYLQPKLLGI LAFFNMQLLS SSVGIEDKKM
1160 1170 1180 1190 1200
ALNSLMSLMK LMGPKHVSSV RVKMMTTLRT GLRFKDDFPE LCCRAWDCFV
1210 1220 1230 1240 1250
RCLDHACLGS LLSHVIVALL PLIHIQPKET AAIFHYLIIE NRDAVQDFLH
1260 1270 1280 1290 1300
EIYFLPDHPE LKKIKAVLQE YRKETSESTD LQTTLQLSMK AIQHENVDVR
1310 1320 1330 1340 1350
IHALTSLKET LYKNQEKLIK YATDSETVEP IISQLVTVLL KGCQDANSQA
1360 1370 1380 1390 1400
RLLCGECLGE LGAIDPGRLD FSTTETQGKD FTFVTGVEDS SFAYGLLMEL
1410 1420 1430 1440 1450
TRAYLAYADN SRAQDSAAYA IQELLSIYDC REMETNGPGH QLWRRFPEHV
1460 1470 1480 1490 1500
REILEPHLNT RYKSSQKSTD WSGVKKPIYL SKLGSNFAEW SASWAGYLIT
1510 1520 1530 1540 1550
KVRHDLASKI FTCCSIMMKH DFKVTIYLLP HILVYVLLGC NQEDQQEVYA
1560 1570 1580 1590 1600
EIMAVLKHDD QHTINTQDIA SDLCQLSTQT VFSMLDHLTQ WARHKFQALK
1610 1620 1630 1640 1650
AEKCPHSKSN RNKVDSMVST VDYEDYQSVT RFLDLIPQDT LAVASFRSKA
1660 1670 1680 1690 1700
YTRAVMHFES FITEKKQNIQ EHLGFLQKLY AAMHEPDGVA GVSAIRKAEP
1710 1720 1730 1740 1750
SLKEQILEHE SLGLLRDATA CYDRAIQLEP DQIIHYHGVV KSMLGLGQLS
1760 1770 1780 1790 1800
TVITQVNGVH ANRSEWTDEL NTYRVEAAWK LSQWDLVENY LAADGKSTTW
1810 1820 1830 1840 1850
SVRLGQLLLS AKKRDITAFY DSLKLVRAEQ IVPLSAASFE RGSYQRGYEY
1860 1870 1880 1890 1900
IVRLHMLCEL EHSIKPLFQH SPGDSSQEDS LNWVARLEMT QNSYRAKEPI
1910 1920 1930 1940 1950
LALRRALLSL NKRPDYNEMV GECWLQSARV ARKAGHHQTA YNALLNAGES
1960 1970 1980 1990 2000
RLAELYVERA KWLWSKGDVH QALIVLQKGV ELCFPENETP PEGKNMLIHG
2010 2020 2030 2040 2050
RAMLLVGRFM EETANFESNA IMKKYKDVTA CLPEWEDGHF YLAKYYDKLM
2060 2070 2080 2090 2100
PMVTDNKMEK QGDLIRYIVL HFGRSLQYGN QFIYQSMPRM LTLWLDYGTK
2110 2120 2130 2140 2150
AYEWEKAGRS DRVQMRNDLG KINKVITEHT NYLAPYQFLT AFSQLISRIC
2160 2170 2180 2190 2200
HSHDEVFVVL MEIIAKVFLA YPQQAMWMMT AVSKSSYPMR VNRCKEILNK
2210 2220 2230 2240 2250
AIHMKKSLEK FVGDATRLTD KLLELCNKPV DGSSSTLSMS THFKMLKKLV
2260 2270 2280 2290 2300
EEATFSEILI PLQSVMIPTL PSILGTHANH ASHEPFPGHW AYIAGFDDMV
2310 2320 2330 2340 2350
EILASLQKPK KISLKGSDGK FYIMMCKPKD DLRKDCRLME FNSLINKCLR
2360 2370 2380 2390 2400
KDAESRRREL HIRTYAVIPL NDECGIIEWV NNTAGLRPIL TKLYKEKGVY
2410 2420 2430 2440 2450
MTGKELRQCM LPKSAALSEK LKVFREFLLP RHPPIFHEWF LRTFPDPTSW
2460 2470 2480 2490 2500
YSSRSAYCRS TAVMSMVGYI LGLGDRHGEN ILFDSLTGEC VHVDFNCLFN
2510 2520 2530 2540 2550
KGETFEVPEI VPFRLTHNMV NGMGPMGTEG LFRRACEVTM RLMRDQREPL
2560 2570 2580 2590 2600
MSVLKTFLHD PLVEWSKPVK GHSKAPLNET GEVVNEKAKT HVLDIEQRLQ
2610 2620 2630 2640 2644
GVIKTRNRVT GLPLSIEGHV HYLIQEATDE NLLCQMYLGW TPYM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3214 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATR
Peptide Modified Sequence
GVELC[+57.0]FPENETPPEGK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
1983
Peptide - Site of Modification
5
Peptide Start
1979
Peptide End
1994
CPTAC ID
CPTAC-3214
Peptide Molecular Mass
1,801.8193
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 33.4 11.3 12.5 33.7 13.7 15.1 47.4 17.8 19.6 15 15 15
y5 (1+) 16.6 11.7 11.3 28.8 16.8 14.7 33.2 20.5 18.5 15 15 15
y4 (1+) 24.5 11 18.1 40.7 17.2 24 47.5 20.4 30.1 15 15 15
y9 (1+) 34.7 20.1 11.8 43.1 22.2 19.7 55.3 29.9 23 15 15 15
y8 (1+) 35.7 24.8 15.7 23.7 26.8 22.3 42.9 36.5 27.3 15 15 15
y10 (1+) 14.9 13.6 10.7 27.9 19.3 16.8 31.6 23.6 19.9 15 15 15
sum 11.2 6.9 9.9 22.4 13.8 14.7 25 15.4 17.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-3215 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATR
Peptide Modified Sequence
GVELC[+57.0]FPENET[+80.0]PPEGK
Modification Type
Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification
1983, 1989
Peptide - Site of Modification
5, 11
Peptide Start
1979
Peptide End
1994
CPTAC ID
CPTAC-3215
Peptide Molecular Mass
1,881.7856
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 16 18.2 12.6 25.9 18.6 19.9 30.4 26 23.6 15 15 15
y9 (1+) 29.3 27.4 23 52.6 28.8 26.1 60.2 39.8 34.8 15 15 15
y10-98 (1+) 30.7 12.3 11.7 37.4 12.1 16.4 48.4 17.3 20.1 15 15 15
y5 (1+) 24.1 12.5 9.5 38 21 13.5 45 24.4 16.5 15 15 15
sum 11.5 11.9 8.8 26.1 16.4 14.1 28.5 20.3 16.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments