| Official Gene Symbol | Other Aliases |
|---|---|
| CHEK1 | CHK1 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 476 | 54434 |
| Protein Name |
|---|
| Serine/threonine-protein kinase Chk1 |
| Sources | |
|---|---|
|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
| 10 | 20 | 30 | 40 | 50 |
| MAVPFVEDWD | LVQTLGEGAY | GEVQLAVNRV | TEEAVAVKIV | DMKRAVDCPE |
| 60 | 70 | 80 | 90 | 100 |
| NIKKEICINK | MLNHENVVKF | YGHRREGNIQ | YLFLEYCSGG | ELFDRIEPDI |
| 110 | 120 | 130 | 140 | 150 |
| GMPEPDAQRF | FHQLMAGVVY | LHGIGITHRD | IKPENLLLDE | RDNLKISDFG |
| 160 | 170 | 180 | 190 | 200 |
| LATVFRYNNR | ERLLNKMCGT | LPYVAPELLK | RREFHAEPVD | VWSCGIVLTA |
| 210 | 220 | 230 | 240 | 250 |
| MLAGELPWDQ | PSDSCQEYSD | WKEKKTYLNP | WKKIDSAPLA | LLHKILVENP |
| 260 | 270 | 280 | 290 | 300 |
| SARITIPDIK | KDRWYNKPLK | KGAKRPRVTS | GGVSESPSGF | SKHIQSNLDF |
| 310 | 320 | 330 | 340 | 350 |
| SPVNSASSEE | NVKYSSSQPE | PRTGLSLWDT | SPSYIDKLVQ | GISFSQPTCP |
| 360 | 370 | 380 | 390 | 400 |
| DHMLLNSQLL | GTPGSSQNPW | QRLVKRMTRF | FTKLDADKSY | QCLKETCEKL |
| 410 | 420 | 430 | 440 | 450 |
| GYQWKKSCMN | QVTISTTDRR | NNKLIFKVNL | LEMDDKILVD | FRLSKGDGLE |
| 460 | 470 | 476 | ||
| FKRHFLKIKG | KLIDIVSSQK | IWLPAT |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
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Data source: Panorama
View Details (opens in a new window)
Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 40.3 | 44.2 | 44.5 | 41.1 | 45.1 | 59 | 57.6 | 63.1 | 73.9 | 15 | 27 | 15 |
| y12 (1+) | 76.4 | 68.8 | 52.7 | 88.1 | 78.3 | 51.3 | 116.6 | 104.2 | 73.5 | 13 | 24 | 13 |
| y9-98 (1+) | 36.5 | 49.2 | 43.5 | 44.4 | 57.3 | 38.3 | 57.5 | 75.5 | 58 | 15 | 26 | 15 |
| y6 (1+) | 32.1 | 45.4 | 44.1 | 42 | 52 | 79.7 | 52.9 | 69 | 91.1 | 15 | 27 | 15 |
| sum | 8.6 | 17.9 | 15.4 | 22.3 | 15 | 19.5 | 23.9 | 23.4 | 24.8 | 15 | 27 | 15 |
Data source: Panorama
View Details (opens in a new window)
Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 16.6 | 13.7 | 8.1 | 25.6 | 14.9 | 6.9 | 30.5 | 20.2 | 10.6 | 12 | 19 | 8 |
| y7 (1+) | 16.9 | 12.5 | 9.7 | 23.9 | 10.6 | 15.5 | 29.3 | 16.4 | 18.3 | 12 | 19 | 8 |
| b5-98 (1+) | 46.6 | 38.9 | 15.6 | 64.4 | 52 | 56 | 79.5 | 64.9 | 58.1 | 12 | 19 | 8 |
| sum | 24.7 | 15.7 | 8.1 | 32.1 | 22 | 17.4 | 40.5 | 27 | 19.2 | 12 | 19 | 8 |
Data source: Panorama
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 47.2 | 26.7 | 34.4 | 51.8 | 29.2 | 32.9 | 70.1 | 39.6 | 47.6 | 15 | 15 | 15 |
| y5 (1+) | 38.7 | 22.7 | 23.5 | 43.2 | 25.7 | 23.3 | 58 | 34.3 | 33.1 | 15 | 15 | 15 |
| y7 (1+) | 29.1 | 11.2 | 15.9 | 28.6 | 20.6 | 14.6 | 40.8 | 23.4 | 21.6 | 15 | 15 | 15 |
| sum | 22.7 | 10.4 | 17.6 | 24.7 | 16.8 | 16.7 | 33.5 | 19.8 | 24.3 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 36.4 | 23.8 | 14.6 | 41.7 | 31.9 | 17.9 | 55.4 | 39.8 | 23.1 | 15 | 15 | 15 |
| y6-98 (1+) | 21.6 | 17.8 | 12.1 | 39.3 | 39.9 | 35.2 | 44.8 | 43.7 | 37.2 | 15 | 15 | 15 |
| y5 (1+) | 18 | 21.9 | 33.7 | 24.3 | 37.6 | 45 | 30.2 | 43.5 | 56.2 | 15 | 15 | 15 |
| y4 (1+) | 12.8 | 6.3 | 17.6 | 23.3 | 17.8 | 20.6 | 26.6 | 18.9 | 27.1 | 15 | 15 | 15 |
| sum | 9.4 | 5.3 | 14.7 | 22.3 | 21.6 | 20.4 | 24.2 | 22.2 | 25.1 | 15 | 15 | 15 |
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