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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

FAAP100

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FAAP100 C17orf70
Sequence Length (AA) Molecular Weight (Da)
881 93433
Protein Name
Fanconi anemia core complex-associated protein 100
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGAAPRVRY LAGFCCPLGG LAAGKPRVLC HEAEVFLSTG SELVYVYDQE
60 70 80 90 100
GGLLTAAFRF PDQVWHLELL APRRLLYALC ARRGLYCLSL DHPGRSRSTS
110 120 130 140 150
QDDRDSEDGD QPSPVIPVDP DACILPDAAL CAFTLLDSVL VTLVQGPARW
160 170 180 190 200
KMQLFEQPCP GEDPRPGGQI GEVELSSYTP PAGVPGKPAA PHFLPVLCSV
210 220 230 240 250
SPSGSRVPHD LLGGSGGFTL EDALFGLLFG ADATLLQSPV VLCGLPDGQL
260 270 280 290 300
CCVILKALVT SRSAPGDPNA LVKILHHLEE PVIFIGALKT EPQAAEAAEN
310 320 330 340 350
FLPDEDVHCD CLVAFGHHGR MLAIKASWDE SGKLVPELRE YCLPGPVLCA
360 370 380 390 400
ACGGGGRVYH STPSDLCVVD LSRGSTPLGP EQPEEGPGGL PPMLCPASLN
410 420 430 440 450
ICSVVSLSAS PRTHEGGTKL LALSAKGRLM TCSLDLDSEM PGPARMTTES
460 470 480 490 500
AGQKIKELLS GIGNISERVS FLKKAVDQRN KALTSLNEAM NVSCALLSSG
510 520 530 540 550
TGPRPISCTT STTWSRLQTQ DVLMATCVLE NSSSFSLDQG WTLCIQVLTS
560 570 580 590 600
SCALDLDSAC SAITYTIPVD QLGPGARREV TLPLGPGENG GLDLPVTVSC
610 620 630 640 650
TLFYSLREVV GGALAPSDSE DPFLDECPSD VLPEQEGVCL PLSRHTVDML
660 670 680 690 700
QCLRFPGLAP PHTRAPSPLG PTRDPVATFL ETCREPGSQP AGPASLRAEY
710 720 730 740 750
LPPSVASIKV SAELLRAALK DGHSGVPLCC ATLQWLLAEN AAVDVVRARA
760 770 780 790 800
LSSIQGVAPD GANVHLIVRE VAMTDLCPAG PIQAVEIQVE SSSLADICRA
810 820 830 840 850
HHAVVGRMQT MVTEQATQGS SAPDLRVQYL RQIHANHETL LREVQTLRDR
860 870 880 881
LCTEDEASSC ATAQRLLQVY RQLRHPSLIL L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3224 Collapse assay details

Data source: Panorama

Official Gene Symbol
FAAP100
Peptide Sequence
APSPLGPTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
665
Peptide End
673
CPTAC ID
CPTAC-3224
Peptide Molecular Mass
894.4923
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5 5.3 4.9 9.1 9.7 6.9 10.4 11.1 8.5 15 15 15
y5 (1+) 15.9 14.6 9.6 17.3 15.9 11.4 23.5 21.6 14.9 15 15 15
y6 (2+) 13.8 4.9 9.4 17.8 8 11.9 22.5 9.4 15.2 15 15 15
sum 5.7 3.5 6.1 7.3 7.1 7.8 9.3 7.9 9.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-3225 Collapse assay details

Data source: Panorama

Official Gene Symbol
FAAP100
Peptide Modified Sequence
APS[+80.0]PLGPTR
Modification Type
Phospho (ST)
Protein - Site of Modification
667
Peptide - Site of Modification
3
Peptide Start
665
Peptide End
673
CPTAC ID
CPTAC-3225
Peptide Molecular Mass
974.4586
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 44.6 14.8 8.7 46.4 15.9 13.5 64.4 21.7 16.1 15 15 15
y7-98 (1+) 29.8 19.7 10.5 34.4 21.8 9.9 45.5 29.4 14.4 15 15 15
y6 (1+) 36 7.4 6.5 43.2 12 9.7 56.2 14.1 11.7 15 15 15
sum 20.1 5.4 4.3 28.2 10.4 6.5 34.6 11.7 7.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments