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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FANCD2 FACD
Sequence Length (AA) Molecular Weight (Da)
1451 164128
Protein Name
Fanconi anemia group D2 protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVSKRRLSKS EDKESLTEDA SKTRKQPLSK KTKKSHIANE VEENDSIFVK
60 70 80 90 100
LLKISGIILK TGESQNQLAV DQIAFQKKLF QTLRRHPSYP KIIEEFVSGL
110 120 130 140 150
ESYIEDEDSF RNCLLSCERL QDEEASMGAS YSKSLIKLLL GIDILQPAII
160 170 180 190 200
KTLFEKLPEY FFENKNSDEI NIPRLIVSQL KWLDRVVDGK DLTTKIMQLI
210 220 230 240 250
SIAPENLQHD IITSLPEILG DSQHADVGKE LSDLLIENTS LTVPILDVLS
260 270 280 290 300
SLRLDPNFLL KVRQLVMDKL SSIRLEDLPV IIKFILHSVT AMDTLEVISE
310 320 330 340 350
LREKLDLQHC VLPSRLQASQ VKLKSKGRAS SSGNQESSGQ SCIILLFDVI
360 370 380 390 400
KSAIRYEKTI SEAWIKAIEN TASVSEHKVF DLVMLFIIYS TNTQTKKYID
410 420 430 440 450
RVLRNKIRSG CIQEQLLQST FSVHYLVLKD MCSSILSLAQ SLLHSLDQSI
460 470 480 490 500
ISFGSLLYKY AFKFFDTYCQ QEVVGALVTH ICSGNEAEVD TALDVLLELV
510 520 530 540 550
VLNPSAMMMN AVFVKGILDY LDNISPQQIR KLFYVLSTLA FSKQNEASSH
560 570 580 590 600
IQDDMHLVIR KQLSSTVFKY KLIGIIGAVT MAGIMAADRS ESPSLTQERA
610 620 630 640 650
NLSDEQCTQV TSLLQLVHSC SEQSPQASAL YYDEFANLIQ HEKLDPKALE
660 670 680 690 700
WVGHTICNDF QDAFVVDSCV VPEGDFPFPV KALYGLEEYD TQDGIAINLL
710 720 730 740 750
PLLFSQDFAK DGGPVTSQES GQKLVSPLCL APYFRLLRLC VERQHNGNLE
760 770 780 790 800
EIDGLLDCPI FLTDLEPGEK LESMSAKERS FMCSLIFLTL NWFREIVNAF
810 820 830 840 850
CQETSPEMKG KVLTRLKHIV ELQIILEKYL AVTPDYVPPL GNFDVETLDI
860 870 880 890 900
TPHTVTAISA KIRKKGKIER KQKTDGSKTS SSDTLSEEKN SECDPTPSHR
910 920 930 940 950
GQLNKEFTGK EEKTSLLLHN SHAFFRELDI EVFSILHCGL VTKFILDTEM
960 970 980 990 1000
HTEATEVVQL GPPELLFLLE DLSQKLESML TPPIARRVPF LKNKGSRNIG
1010 1020 1030 1040 1050
FSHLQQRSAQ EIVHCVFQLL TPMCNHLENI HNYFQCLAAE NHGVVDGPGV
1060 1070 1080 1090 1100
KVQEYHIMSS CYQRLLQIFH GLFAWSGFSQ PENQNLLYSA LHVLSSRLKQ
1110 1120 1130 1140 1150
GEHSQPLEEL LSQSVHYLQN FHQSIPSFQC ALYLIRLLMV ILEKSTASAQ
1160 1170 1180 1190 1200
NKEKIASLAR QFLCRVWPSG DKEKSNISND QLHALLCIYL EHTESILKAI
1210 1220 1230 1240 1250
EEIAGVGVPE LINSPKDASS STFPTLTRHT FVVFFRVMMA ELEKTVKKIE
1260 1270 1280 1290 1300
PGTAADSQQI HEEKLLYWNM AVRDFSILIN LIKVFDSHPV LHVCLKYGRL
1310 1320 1330 1340 1350
FVEAFLKQCM PLLDFSFRKH REDVLSLLET FQLDTRLLHH LCGHSKIHQD
1360 1370 1380 1390 1400
TRLTQHVPLL KKTLELLVCR VKAMLTLNNC REAFWLGNLK NRDLQGEEIK
1410 1420 1430 1440 1450
SQNSQESTAD ESEDDMSSQA SKSKATEDGE EDEVSAGEKE QDSDESYDDS
1451
D

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3227 Collapse assay details

Data source: Panorama

Official Gene Symbol
FANCD2
Peptide Sequence
KQLSSTVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
561
Peptide End
569
CPTAC ID
CPTAC-3227
Peptide Molecular Mass
1,036.5917
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 25.3 9.6 9.6 29 14.1 11.1 38.5 17.1 14.7 15 15 15
y5 (1+) 28.7 26.3 20.8 50.1 27.9 20.8 57.7 38.3 29.4 15 15 15
y7 (1+) 16.4 6.4 7 16.1 10.3 11.9 23 12.1 13.8 15 15 15
sum 12.4 4.8 6.5 14.3 10.7 10 18.9 11.7 11.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3228 Collapse assay details

Data source: Panorama

Official Gene Symbol
FANCD2
Peptide Modified Sequence
K[+114.0]QLSSTVFK
Modification Type
Ubiquitin
Protein - Site of Modification
561
Peptide - Site of Modification
1
Peptide Start
561
Peptide End
569
CPTAC ID
CPTAC-3228
Peptide Molecular Mass
1,150.6347
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted mass spectrometry enables robust quantification of FANCD2 mono-ubiquitination in response to DNA damage. Whiteaker JR, Zhao L, Ivey RG, Sanchez-Bonilla M, Moore HD, Schoenherr RM, Yan P, Lin C, Shimamura A, Paulovich AG. DNA Repair (Amst). 2018 May;65:47-53. doi: 10.1016/j.dnarep.2018.03.003. Epub 2018 Mar 21.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 19.9 16.8 18.4 26.5 36 36.3 33.1 39.7 40.7 15 15 15
b6 (1+) 34.4 23.2 21.1 50.7 30.8 28.6 61.3 38.6 35.5 15 15 15
y7 (1+) 9.2 8.5 7.5 23.2 8.2 8.2 25 11.8 11.1 15 15 15
sum 6.8 6.7 5.5 16.7 10 11.2 18 12 12.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3229 Collapse assay details

Data source: Panorama

Official Gene Symbol
FANCD2
Peptide Sequence
QLSSTVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
562
Peptide End
569
CPTAC ID
CPTAC-3229
Peptide Molecular Mass
908.4967
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted mass spectrometry enables robust quantification of FANCD2 mono-ubiquitination in response to DNA damage. Whiteaker JR, Zhao L, Ivey RG, Sanchez-Bonilla M, Moore HD, Schoenherr RM, Yan P, Lin C, Shimamura A, Paulovich AG. DNA Repair (Amst). 2018 May;65:47-53. doi: 10.1016/j.dnarep.2018.03.003. Epub 2018 Mar 21.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 10.8 6.7 10.2 11.7 11 12.4 15.9 12.9 16.1 15 15 15
y6 (1+) 13.5 14.3 11.5 13.7 20.9 14.1 19.2 25.3 18.2 15 15 15
y6 (2+) 19.3 8.6 7.9 20 10 10.2 27.8 13.2 12.9 15 15 15
sum 9.5 8.3 7.7 8.8 12.9 8.3 12.9 15.3 11.3 15 15 15

Additional Resources and Comments