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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

RAD51C

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RAD51C RAD51L2
Sequence Length (AA) Molecular Weight (Da)
376 42190
Protein Name
DNA repair protein RAD51 homolog 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRGKTFRFEM QRDLVSFPLS PAVRVKLVSA GFQTAEELLE VKPSELSKEV
60 70 80 90 100
GISKAEALET LQIIRRECLT NKPRYAGTSE SHKKCTALEL LEQEHTQGFI
110 120 130 140 150
ITFCSALDDI LGGGVPLMKT TEICGAPGVG KTQLCMQLAV DVQIPECFGG
160 170 180 190 200
VAGEAVFIDT EGSFMVDRVV DLATACIQHL QLIAEKHKGE EHRKALEDFT
210 220 230 240 250
LDNILSHIYY FRCRDYTELL AQVYLLPDFL SEHSKVRLVI VDGIAFPFRH
260 270 280 290 300
DLDDLSLRTR LLNGLAQQMI SLANNHRLAV ILTNQMTTKI DRNQALLVPA
310 320 330 340 350
LGESWGHAAT IRLIFHWDRK QRLATLYKSP SQKECTVLFQ IKPQGFRDTV
360 370 376
VTSACSLQTE GSLSTRKRSR DPEEEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3250 Collapse assay details

Data source: Panorama

Official Gene Symbol
RAD51C
Peptide Sequence
DLVSFPLSPAVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
13
Peptide End
24
CPTAC ID
CPTAC-3250
Peptide Molecular Mass
1,299.7187
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.3 10.9 6.3 12.8 17.3 7.4 14.7 20.4 9.7 15 15 15
y4 (1+) 24.1 10.9 6.2 28.3 15.2 12.4 37.2 18.7 13.9 15 15 15
y9 (1+) 12.5 5 11.6 15.3 11 13.5 19.8 12.1 17.8 15 15 15
y8 (1+) 17.2 9.9 10.7 20.6 14.9 12.3 26.8 17.9 16.3 15 15 15
sum 6 7.3 6.2 10.7 13.4 8.9 12.3 15.3 10.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-3287 Collapse assay details

Data source: Panorama

Official Gene Symbol
RAD51C
Peptide Modified Sequence
DLVSFPLS[+80.0]PAVR
Modification Type
Phospho (ST)
Protein - Site of Modification
20
Peptide - Site of Modification
8
Peptide Start
13
Peptide End
24
CPTAC ID
CPTAC-3287
Peptide Molecular Mass
1,379.6850
Species
Homo sapiens (Human)
Assay Type
Enrichment ImmunoMRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8-98 (1+) 32.6 19.7 17.4 35.7 31 28.7 48.3 36.7 33.6 15 15 15
y7-98 (1+) 11.1 19.3 14.9 18 28.2 25.7 21.1 34.2 29.7 15 15 15
y9-98 (1+) 20.7 11.7 15.4 27.4 25.6 23.2 34.3 28.1 27.8 15 15 15
y8 (1+) 22.1 11.6 17.2 24.8 26.7 22.6 33.2 29.1 28.4 15 15 15
y9 (1+) 10.8 10.6 16.3 24.8 23.3 26.3 27 25.6 30.9 15 15 15
sum 10.1 10.3 14.1 14.4 23.7 24.1 17.6 25.8 27.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments