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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CKAP4 N/A
Sequence Length (AA) Molecular Weight (Da)
602 66022
Protein Name
Cytoskeleton-associated protein 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPSAKQRGSK GGHGAASPSE KGAHPSGGAD DVAKKPPPAP QQPPPPPAPH
60 70 80 90 100
PQQHPQQHPQ NQAHGKGGHR GGGGGGGKSS SSSSASAAAA AAAASSSASC
110 120 130 140 150
SRRLGRALNF LFYLALVAAA AFSGWCVHHV LEEVQQVRRS HQDFSRQREE
160 170 180 190 200
LGQGLQGVEQ KVQSLQATFG TFESILRSSQ HKQDLTEKAV KQGESEVSRI
210 220 230 240 250
SEVLQKLQNE ILKDLSDGIH VVKDARERDF TSLENTVEER LTELTKSIND
260 270 280 290 300
NIAIFTEVQK RSQKEINDMK AKVASLEESE GNKQDLKALK EAVKEIQTSA
310 320 330 340 350
KSREWDMEAL RSTLQTMESD IYTEVRELVS LKQEQQAFKE AADTERLALQ
360 370 380 390 400
ALTEKLLRSE ESVSRLPEEI RRLEEELRQL KSDSHGPKED GGFRHSEAFE
410 420 430 440 450
ALQQKSQGLD SRLQHVEDGV LSMQVASARQ TESLESLLSK SQEHEQRLAA
460 470 480 490 500
LQGRLEGLGS SEADQDGLAS TVRSLGETQL VLYGDVEELK RSVGELPSTV
510 520 530 540 550
ESLQKVQEQV HTLLSQDQAQ AARLPPQDFL DRLSSLDNLK ASVSQVEADL
560 570 580 590 600
KMLRTAVDSL VAYSVKIETN ENNLESAKGL LDDLRNDLDR LFVKVEKIHE
602
KV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-335 Collapse assay details

Data source: Panorama

Official Gene Symbol
CKAP4
Peptide Sequence
LEGLGSSEADQDGLASTVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
455
Peptide End
473
CPTAC ID
CPTAC-335
Peptide Molecular Mass
1,903.9123
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 9.2 6.6 4.9 15.2 12.8 7.2 17.8 14.4 8.7 15 25 15
y10 (1+) 12.9 10.9 8.9 13.9 11.3 13.1 19 15.7 15.8 15 25 15
y8 (1+) 16.9 8.3 8.4 21.7 9.2 6.9 27.5 12.4 10.9 15 25 15
sum 7.3 6.1 4.5 11 9.2 5.7 13.2 11 7.3 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-336 Collapse assay details

Data source: Panorama

Official Gene Symbol
CKAP4
Peptide Sequence
TAVDSLVAYSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
555
Peptide End
566
CPTAC ID
CPTAC-336
Peptide Molecular Mass
1,251.6711
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 7.3 8.5 7.2 10.3 9.8 8.6 12.6 13 11.2 15 25 15
y9 (1+) 5.6 6 4.7 9.8 7.3 6.9 11.3 9.4 8.3 15 25 15
y6 (1+) 5.3 9.7 7.5 10.9 9 9.9 12.1 13.2 12.4 15 25 15
sum 3.2 5.7 4.1 8.5 6.9 6.4 9.1 8.9 7.6 15 25 15

Additional Resources and Comments