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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CORO1B N/A
Sequence Length (AA) Molecular Weight (Da)
489 54235
Protein Name
Coronin-1B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSFRKVVRQS KFRHVFGQPV KNDQCYEDIR VSRVTWDSTF CAVNPKFLAV
60 70 80 90 100
IVEASGGGAF LVLPLSKTGR IDKAYPTVCG HTGPVLDIDW CPHNDEVIAS
110 120 130 140 150
GSEDCTVMVW QIPENGLTSP LTEPVVVLEG HTKRVGIIAW HPTARNVLLS
160 170 180 190 200
AGCDNVVLIW NVGTAEELYR LDSLHPDLIY NVSWNHNGSL FCSACKDKSV
210 220 230 240 250
RIIDPRRGTL VAEREKAHEG ARPMRAIFLA DGKVFTTGFS RMSERQLALW
260 270 280 290 300
DPENLEEPMA LQELDSSNGA LLPFYDPDTS VVYVCGKGDS SIRYFEITEE
310 320 330 340 350
PPYIHFLNTF TSKEPQRGMG SMPKRGLEVS KCEIARFYKL HERKCEPIVM
360 370 380 390 400
TVPRKSDLFQ DDLYPDTAGP EAALEAEEWV SGRDADPILI SLREAYVPSK
410 420 430 440 450
QRDLKISRRN VLSDSRPAMA PGSSHLGAPA STTTAADATP SGSLARAGEA
460 470 480 489
GKLEEVMQEL RALRALVKEQ GDRICRLEEQ LGRMENGDA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-340 Collapse assay details

Data source: Panorama

Official Gene Symbol
CORO1B
Peptide Sequence
DADPILISLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
384
Peptide End
393
CPTAC ID
CPTAC-340
Peptide Molecular Mass
1,111.6237
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 9.1 15.7 5 19.9 24.9 22.4 21.9 29.4 23 15 25 15
y6 (1+) 11.9 10.3 5 14.2 11.6 7.5 18.5 15.5 9 15 25 15
y5 (1+) 7.5 6.1 3.9 10.3 8.2 7.6 12.7 10.2 8.5 15 25 15
sum 6.8 9.5 4.1 9.1 13.8 11.7 11.4 16.8 12.4 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-339 Collapse assay details

Data source: Panorama

Official Gene Symbol
CORO1B
Peptide Modified Sequence
VTWDSTFC[+57.0]AVNPK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
41
Peptide - Site of Modification
8
Peptide Start
34
Peptide End
46
CPTAC ID
CPTAC-339
Peptide Molecular Mass
1,523.7079
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 10.3 8.4 7.8 10.8 12.7 11.1 14.9 15.2 13.6 15 25 15
y9 (1+) 8.9 9.4 8.3 8.5 10.7 5.7 12.3 14.2 10.1 15 25 15
y7 (1+) 13.2 8.7 5.2 15.3 9 5.3 20.2 12.5 7.4 15 25 15
sum 9.3 8.1 6 8.6 9.3 6.9 12.7 12.3 9.1 15 25 15

Additional Resources and Comments