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Overview Data source: UniProt

Official Gene Symbol Other Aliases
DDOST KIAA0115, OST48
Sequence Length (AA) Molecular Weight (Da)
456 50801
Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGYFRCARAG SFGRRRKMEP STAARAWALF WLLLPLLGAV CASGPRTLVL
60 70 80 90 100
LDNLNVRETH SLFFRSLKDR GFELTFKTAD DPSLSLIKYG EFLYDNLIIF
110 120 130 140 150
SPSVEDFGGN INVETISAFI DGGGSVLVAA SSDIGDPLRE LGSECGIEFD
160 170 180 190 200
EEKTAVIDHH NYDISDLGQH TLIVADTENL LKAPTIVGKS SLNPILFRGV
210 220 230 240 250
GMVADPDNPL VLDILTGSST SYSFFPDKPI TQYPHAVGKN TLLIAGLQAR
260 270 280 290 300
NNARVIFSGS LDFFSDSFFN SAVQKAAPGS QRYSQTGNYE LAVALSRWVF
310 320 330 340 350
KEEGVLRVGP VSHHRVGETA PPNAYTVTDL VEYSIVIQQL SNGKWVPFDG
360 370 380 390 400
DDIQLEFVRI DPFVRTFLKK KGGKYSVQFK LPDVYGVFQF KVDYNRLGYT
410 420 430 440 450
HLYSSTQVSV RPLQHTQYER FIPSAYPYYA SAFSMMLGLF IFSIVFLHMK
456
EKEKSD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-350 Collapse assay details

Data source: Panorama

Official Gene Symbol
DDOST
Peptide Sequence
TADDPSLSLIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
78
Peptide End
88
CPTAC ID
CPTAC-350
Peptide Molecular Mass
1,158.6132
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.1 12.3 12.3 20 14.8 18.9 23.4 19.2 22.5 15 25 15
y8 (1+) 17.7 12.8 6.6 25.4 13.8 11.8 31 18.8 13.5 15 25 15
y7 (1+) 6 9 4.6 5.9 10 7.8 8.4 13.5 9.1 15 25 15
sum 7 9.2 4.8 10.1 10.2 9.4 12.3 13.7 10.6 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-349 Collapse assay details

Data source: Panorama

Official Gene Symbol
DDOST
Peptide Sequence
TLVLLDNLNVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
47
Peptide End
57
CPTAC ID
CPTAC-349
Peptide Molecular Mass
1,268.7452
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 10.1 7.3 6.1 27.8 11.1 14.9 29.6 13.3 16.1 15 25 15
y7 (1+) 13.8 6.5 7.8 28.8 10.6 12.4 31.9 12.4 14.6 15 25 15
y6 (1+) 21.9 7.3 6.1 39 11.1 16 44.7 13.3 17.1 15 25 15
sum 13.7 6.5 5.6 29.2 10.8 13.3 32.3 12.6 14.4 15 25 15

Additional Resources and Comments