Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ERP44 KIAA0573,TXNDC4
Sequence Length (AA) Molecular Weight (Da)
406 46971
Protein Name
Endoplasmic reticulum resident protein 44
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHPAVFLSLP DLRCSLLLLV TWVFTPVTTE ITSLDTENID EILNNADVAL
60 70 80 90 100
VNFYADWCRF SQMLHPIFEE ASDVIKEEFP NENQVVFARV DCDQHSDIAQ
110 120 130 140 150
RYRISKYPTL KLFRNGMMMK REYRGQRSVK ALADYIRQQK SDPIQEIRDL
160 170 180 190 200
AEITTLDRSK RNIIGYFEQK DSDNYRVFER VANILHDDCA FLSAFGDVSK
210 220 230 240 250
PERYSGDNII YKPPGHSAPD MVYLGAMTNF DVTYNWIQDK CVPLVREITF
260 270 280 290 300
ENGEELTEEG LPFLILFHMK EDTESLEIFQ NEVARQLISE KGTINFLHAD
310 320 330 340 350
CDKFRHPLLH IQKTPADCPV IAIDSFRHMY VFGDFKDVLI PGKLKQFVFD
360 370 380 390 400
LHSGKLHREF HHGPDPTDTA PGEQAQDVAS SPPESSFQKL APSEYRYTLL
406
RDRDEL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-363 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERP44
Peptide Sequence
DLAEITTLDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
149
Peptide End
158
CPTAC ID
CPTAC-363
Peptide Molecular Mass
1,145.5928
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) NA 2.8 6.6 NA NA 4.6 NA NA 8 0 5 6
y6 (1+) 7.8 6.5 3.6 9.5 7.3 9.8 12.3 9.8 10.4 15 25 15
y5 (1+) 4.8 7.3 6.2 7.3 8.6 8.1 8.7 11.3 10.2 15 25 15
sum 5.8 6 3.6 7.1 6.5 5.6 9.2 8.8 6.7 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-364 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERP44
Peptide Modified Sequence
TPADC[+57.0]PVIAIDSFR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
318
Peptide - Site of Modification
5
Peptide Start
314
Peptide End
327
CPTAC ID
CPTAC-364
Peptide Molecular Mass
1,560.7606
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 11.9 12.1 8.3 16.2 12.4 7.9 20.1 17.3 11.5 15 25 15
y9 (1+) 5.9 10.6 5.7 15.6 9.9 6.4 16.7 14.5 8.6 15 25 15
y7 (1+) 17.9 13.1 14.8 21.6 13.2 15.4 28.1 18.6 21.4 15 25 15
sum 5.6 9.4 7.6 13.9 9.2 6.9 15 13.2 10.3 15 25 15

Additional Resources and Comments