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Overview Data source: UniProt

Official Gene Symbol Other Aliases
LRRC59 N/A
Sequence Length (AA) Molecular Weight (Da)
307 34930
Protein Name
Leucine-rich repeat-containing protein 59
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTKAGSKGGN LRDKLDGNEL DLSLSDLNEV PVKELAALPK ATILDLSCNK
60 70 80 90 100
LTTLPSDFCG LTHLVKLDLS KNKLQQLPAD FGRLVNLQHL DLLNNKLVTL
110 120 130 140 150
PVSFAQLKNL KWLDLKDNPL DPVLAKVAGD CLDEKQCKQC ANKVLQHMKA
160 170 180 190 200
VQADQERERQ RRLEVEREAE KKREAKQRAK EAQERELRKR EKAEEKERRR
210 220 230 240 250
KEYDALKAAK REQEKKPKKE ANQAPKSKSG SRPRKPPPRK HTRSWAVLKL
260 270 280 290 300
LLLLLLFGVA GGLVACRVTE LQQQPLCTSV NTIYDNAVQG LRRHEILQWV
307
LQTDSQQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-401 Collapse assay details

Data source: Panorama

Official Gene Symbol
LRRC59
Peptide Modified Sequence
ATILDLSC[+57.0]NK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
48
Peptide - Site of Modification
8
Peptide Start
41
Peptide End
50
CPTAC ID
CPTAC-401
Peptide Molecular Mass
1,133.5751
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.5 5.5 2.4 6.8 6.6 5.5 9.4 8.6 6 15 25 15
y6 (1+) 6.8 5.8 3.5 8.1 8 4.1 10.6 9.9 5.4 15 25 15
y5 (1+) 16.5 10.4 2.9 24.2 10.4 3.1 29.3 14.7 4.2 15 25 15
sum 6.2 5.5 2.4 6.1 7.2 3.8 8.7 9.1 4.5 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-402 Collapse assay details

Data source: Panorama

Official Gene Symbol
LRRC59
Peptide Sequence
LVNLQHLDLLNNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
84
Peptide End
96
CPTAC ID
CPTAC-402
Peptide Molecular Mass
1,532.8675
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 51.6 59.2 21 63 63.5 69.7 81.4 86.8 72.8 15 25 15
y11 (2+) 6.4 7.5 5.1 8.5 13.6 7.8 10.6 15.5 9.3 15 25 15
y9 (2+) 19.8 15.7 6.4 24.1 19.4 10.3 31.2 25 12.1 15 25 15
sum 5.6 7.1 4.7 8.5 13.2 6.6 10.2 15 8.1 15 25 15

Additional Resources and Comments