Overview Data source: UniProt

Official Gene Symbol Other Aliases
MANF ARMET,ARP
Sequence Length (AA) Molecular Weight (Da)
182 20700
Protein Name
Mesencephalic astrocyte-derived neurotrophic factor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRRMWATQGL AVALALSVLP GSRALRPGDC EVCISYLGRF YQDLKDRDVT
60 70 80 90 100
FSPATIENEL IKFCREARGK ENRLCYYIGA TDDAATKIIN EVSKPLAHHI
110 120 130 140 150
PVEKICEKLK KKDSQICELK YDKQIDLSTV DLKKLRVKEL KKILDDWGET
160 170 180 182
CKGCAEKSDY IRKINELMPK YAPKAASART DL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-404 Collapse assay details

Data source: Panorama

Official Gene Symbol
MANF
Peptide Modified Sequence
ILDDWGETC[+57.0]K
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
151
Peptide - Site of Modification
9
Peptide Start
143
Peptide End
152
CPTAC ID
CPTAC-404
Peptide Molecular Mass
1,235.5492
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 6.9 35.8 5.1 9.1 59.5 8.2 11.4 69.4 9.7 25 15 15
y7 (1+) 11.4 32 7.7 15.3 32.2 20.3 19.1 45.4 21.7 25 15 15
y6 (1+) 8.2 29.7 7.6 11.1 42.2 8.7 13.8 51.6 11.6 25 15 15
sum 7.2 32.5 4.4 8.6 48.8 8.9 11.2 58.6 9.9 25 15 15

Additional Resources and Comments


Assay Details for CPTAC-403 Collapse assay details

Data source: Panorama

Official Gene Symbol
MANF
Peptide Modified Sequence
LC[+57.0]YYIGATDDAATK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
75
Peptide - Site of Modification
2
Peptide Start
74
Peptide End
87
CPTAC ID
CPTAC-403
Peptide Molecular Mass
1,560.7130
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 9.5 14.6 6.6 18.3 17.2 15.9 20.6 22.6 17.2 15 25 15
y10 (1+) 12.7 11.1 9.7 16.1 13.4 12 20.5 17.4 15.4 15 25 15
y9 (1+) 8.8 8.8 6.5 10.6 10.6 6.9 13.8 13.8 9.5 15 25 15
sum 6.9 9.4 5.9 7.8 11 7.7 10.4 14.5 9.7 15 25 15

Additional Resources and Comments