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Overview Data source: UniProt

Official Gene Symbol Other Aliases
NASP N/A
Sequence Length (AA) Molecular Weight (Da)
788 85238
Protein Name
Nuclear autoantigenic sperm protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAMESTATAA VAAELVSADK IEDVPAPSTS ADKVESLDVD SEAKKLLGLG
60 70 80 90 100
QKHLVMGDIP AAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR
110 120 130 140 150
MENGVLGNAL EGVHVEEEEG EKTEDESLVE NNDNIDEEAR EELREQVYDA
160 170 180 190 200
MGEKEEAKKT EDKSLAKPET DKEQDSEMEK GGREDMDISK SAEEPQEKVD
210 220 230 240 250
LTLDWLTETS EEAKGGAAPE GPNEAEVTSG KPEQEVPDAE EEKSVSGTDV
260 270 280 290 300
QEECREKGGQ EKQGEVIVSI EEKPKEVSEE QPVVTLEKQG TAVEVEAESL
310 320 330 340 350
DPTVKPVDVG GDEPEEKVVT SENEAGKAVL EQLVGQEVPP AEESPEVTTE
360 370 380 390 400
AAEASAVEAG SEVSEKPGQE APVLPKDGAV NGPSVVGDQT PIEPQTSIER
410 420 430 440 450
LTETKDGSGL EEKVRAKLVP SQEETKLSVE ESEAAGDGVD TKVAQGATEK
460 470 480 490 500
SPEDKVQIAA NEETQEREEQ MKEGEETEGS EEDDKENDKT EEMPNDSVLE
510 520 530 540 550
NKSLQENEEE EIGNLELAWD MLDLAKIIFK RQETKEAQLY AAQAHLKLGE
560 570 580 590 600
VSVESENYVQ AVEEFQSCLN LQEQYLEAHD RLLAETHYQL GLAYGYNSQY
610 620 630 640 650
DEAVAQFSKS IEVIENRMAV LNEQVKEAEG SSAEYKKEIE ELKELLPEIR
660 670 680 690 700
EKIEDAKESQ RSGNVAELAL KATLVESSTS GFTPGGGGSS VSMIASRKPT
710 720 730 740 750
DGASSSNCVT DISHLVRKKR KPEEESPRKD DAKKAKQEPE VNGGSGDAVP
760 770 780 788
SGNEVSENME EEAENQAESR AAVEGTVEAG ATVESTAC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-411 Collapse assay details

Data source: Panorama

Official Gene Symbol
NASP
Peptide Sequence
LSVEESEAAGDGVDTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
427
Peptide End
442
CPTAC ID
CPTAC-411
Peptide Molecular Mass
1,605.7370
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 9.5 10.7 8.6 13.4 16.7 12.1 16.4 19.8 14.8 15 25 15
y11 (1+) 12.7 9.8 7.9 15.3 14.7 16.6 19.9 17.7 18.4 15 25 15
y9 (1+) 8 7.8 7.6 11.8 12.2 9.3 14.3 14.5 12 15 25 15
sum 6.8 7.3 5.7 10.7 13.2 8.7 12.7 15.1 10.4 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-412 Collapse assay details

Data source: Panorama

Official Gene Symbol
NASP
Peptide Sequence
VQIAANEETQER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
456
Peptide End
467
CPTAC ID
CPTAC-412
Peptide Molecular Mass
1,386.6739
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 9.8 5.1 2.3 16.8 11.7 10.8 19.4 12.8 11 15 15 15
y9 (1+) 19.9 5.5 2.3 19 11.9 10.6 27.5 13.1 10.8 15 15 15
y8 (1+) 18.1 6.4 1.5 26.9 12.4 11.8 32.4 14 11.9 15 15 15
sum 14 5.5 1.9 14.7 11.8 10.9 20.3 13 11.1 15 15 15

Additional Resources and Comments