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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PSMD5 KIAA0072
Sequence Length (AA) Molecular Weight (Da)
504 56196
Protein Name
26S proteasome non-ATPase regulatory subunit 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAQALALLR EVARLEAPLE ELRALHSVLQ AVPLNELRQQ AAELRLGPLF
60 70 80 90 100
SLLNENHREK TTLCVSILER LLQAMEPVHV ARNLRVDLQR GLIHPDDSVK
110 120 130 140 150
ILTLSQIGRI VENSDAVTEI LNNAELLKQI VYCIGGENLS VAKAAIKSLS
160 170 180 190 200
RISLTQAGLE ALFESNLLDD LKSVMKTNDI VRYRVYELII EISSVSPESL
210 220 230 240 250
NYCTTSGLVT QLLRELTGED VLVRATCIEM VTSLAYTHHG RQYLAQEGVI
260 270 280 290 300
DQISNIIVGA DSDPFSSFYL PGFVKFFGNL AVMDSPQQIC ERYPIFVEKV
310 320 330 340 350
FEMIESQDPT MIGVAVDTVG ILGSNVEGKQ VLQKTGTRFE RLLMRIGHQS
360 370 380 390 400
KNAPVELKIR CLDAISSLLY LPPEQQTDDL LRMTESWFSS LSRDPLELFR
410 420 430 440 450
GISSQPFPEL HCAALKVFTA IANQPWAQKL MFNSPGFVEY VVDRSVEHDK
460 470 480 490 500
ASKDAKYELV KALANSKTIA EIFGNPNYLR LRTYLSEGPY YVKPVSTTAV
504
EGAE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-429 Collapse assay details

Data source: Panorama

Official Gene Symbol
PSMD5
Peptide Sequence
TIAEIFGNPNYLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
468
Peptide End
480
CPTAC ID
CPTAC-429
Peptide Molecular Mass
1,506.7831
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 13.8 12.6 12.5 18.2 15.3 15.3 22.8 19.8 19.8 15 25 15
y8 (1+) 7.9 8.4 7.5 15.6 9.5 8.8 17.5 12.7 11.6 15 25 15
y7 (1+) 9.7 7.3 6.6 11.5 9.3 7.7 15 11.8 10.1 15 25 15
sum 5.9 6.1 5.5 12.5 8.6 6.6 13.8 10.5 8.6 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-428 Collapse assay details

Data source: Panorama

Official Gene Symbol
PSMD5
Peptide Sequence
VFTAIANQPWAQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
417
Peptide End
429
CPTAC ID
CPTAC-428
Peptide Molecular Mass
1,472.7776
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 8.6 10.3 5.7 13.3 16.3 22.7 15.8 19.3 23.4 15 25 15
y7 (1+) 8.5 7.5 7.2 9.2 8.2 7.2 12.5 11.1 10.2 15 25 15
y5 (1+) 9.3 8.1 5.1 8 10.3 9.8 12.3 13.1 11 15 25 15
sum 6.2 6.1 3.6 7.3 9 9.5 9.6 10.9 10.2 15 25 15

Additional Resources and Comments