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Overview Data source: UniProt

Official Gene Symbol Other Aliases
RPL30 N/A
Sequence Length (AA) Molecular Weight (Da)
115 12784
Protein Name
60S ribosomal protein L30
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN
60 70 80 90 100
NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYYR VCTLAIIDPG
110 115
DSDIIRSMPE QTGEK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-434 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPL30
Peptide Modified Sequence
LVILANNC[+57.0]PALR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
52
Peptide - Site of Modification
8
Peptide Start
45
Peptide End
56
CPTAC ID
CPTAC-434
Peptide Molecular Mass
1,352.7598
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 7.3 7.2 5.2 7.2 9 7.9 10.3 11.5 9.5 15 25 15
y8 (1+) 5.7 6.8 5.2 6.9 8.3 5.9 8.9 10.7 7.9 15 25 15
y7 (1+) 6.5 6 4.6 7.3 7.2 5 9.8 9.4 6.8 15 25 15
sum 5.6 6.3 4.8 5.8 7.7 5.7 8.1 9.9 7.5 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-435 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPL30
Peptide Modified Sequence
VC[+57.0]TLAIIDPGDSDIIR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
92
Peptide - Site of Modification
2
Peptide Start
91
Peptide End
106
CPTAC ID
CPTAC-435
Peptide Molecular Mass
1,756.9029
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 9.8 8.6 8.1 12.2 10.9 16.9 15.6 13.9 18.7 25 15 15
y10 (1+) 9.3 8.6 6.8 12.9 9.6 15.7 15.9 12.9 17.1 25 15 15
y9 (1+) 8.9 9.3 4.4 13.6 10.5 13.1 16.3 14 13.8 25 15 15
sum 8.4 8.3 5.9 12.3 10 14.7 14.9 13 15.8 25 15 15

Additional Resources and Comments