SARS, CPTAC-438 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SARS	SERS

Sequence Length (AA)	Molecular Weight (Da)
514	58777

Protein Name
Serine--tRNA ligase, cytoplasmic

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVLDLDLFRV	DKGGDPALIR	ETQEKRFKDP	GLVDQLVKAD	SEWRRCRFRA
60	70	80	90	100
DNLNKLKNLC	SKTIGEKMKK	KEPVGDDESV	PENVLSFDDL	TADALANLKV
110	120	130	140	150
SQIKKVRLLI	DEAILKCDAE	RIKLEAERFE	NLREIGNLLH	PSVPISNDED
160	170	180	190	200
VDNKVERIWG	DCTVRKKYSH	VDLVVMVDGF	EGEKGAVVAG	SRGYFLKGVL
210	220	230	240	250
VFLEQALIQY	ALRTLGSRGY	IPIYTPFFMR	KEVMQEVAQL	SQFDEELYKV
260	270	280	290	300
IGKGSEKSDD	NSYDEKYLIA	TSEQPIAALH	RDEWLRPEDL	PIKYAGLSTC
310	320	330	340	350
FRQEVGSHGR	DTRGIFRVHQ	FEKIEQFVYS	SPHDNKSWEM	FEEMITTAEE
360	370	380	390	400
FYQSLGIPYH	IVNIVSGSLN	HAASKKLDLE	AWFPGSGAFR	ELVSCSNCTD
410	420	430	440	450
YQARRLRIRY	GQTKKMMDKV	EFVHMLNATM	CATTRTICAI	LENYQTEKGI
460	470	480	490	500
TVPEKLKEFM	PPGLQELIPF	VKPAPIEQEP	SKKQKKQHEG	SKKKAAARDV
510	514
TLENRLQNME	VTDA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-438 Collapse assay details

Data source: Panorama

Official Gene Symbol: SARS
Peptide Sequence: DEWLRPEDLPIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 282
Peptide End: 293

CPTAC ID: CPTAC-438
Peptide Molecular Mass: 1,509.7827
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Broad Institute and FHCRC
Submitting Lab PI: Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	16.9	36.9	21.2	35.6	46.2	33.3	39.4	59.1	39.5	15	25	15
y10 (2+)	7.2	6.2	9.4	25.6	14	11	26.6	15.3	14.5	15	25	15
y7 (2+)	31.9	26.8	30.4	43.6	30.5	46.9	54	40.6	55.9	15	25	15
sum	7.9	6.1	9.3	25.3	14.7	9.6	26.5	15.9	13.4	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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