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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ZYX N/A
Sequence Length (AA) Molecular Weight (Da)
572 61277
Protein Name
Zyxin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAPRPSPAI SVSVSAPAFY APQKKFGPVV APKPKVNPFR PGDSEPPPAP
60 70 80 90 100
GAQRAQMGRV GEIPPPPPED FPLPPPPLAG DGDDAEGALG GAFPPPPPPI
110 120 130 140 150
EESFPPAPLE EEIFPSPPPP PEEEGGPEAP IPPPPQPREK VSSIDLEIDS
160 170 180 190 200
LSSLLDDMTK NDPFKARVSS GYVPPPVATP FSSKSSTKPA AGGTAPLPPW
210 220 230 240 250
KSPSSSQPLP QVPAPAQSQT QFHVQPQPQP KPQVQLHVQS QTQPVSLANT
260 270 280 290 300
QPRGPPASSP APAPKFSPVT PKFTPVASKF SPGAPGGSGS QPNQKLGHPE
310 320 330 340 350
ALSAGTGSPQ PPSFTYAQQR EKPRVQEKQH PVPPPAQNQN QVRSPGAPGP
360 370 380 390 400
LTLKEVEELE QLTQQLMQDM EHPQRQNVAV NELCGRCHQP LARAQPAVRA
410 420 430 440 450
LGQLFHIACF TCHQCAQQLQ GQQFYSLEGA PYCEGCYTDT LEKCNTCGEP
460 470 480 490 500
ITDRMLRATG KAYHPHCFTC VVCARPLEGT SFIVDQANRP HCVPDYHKQY
510 520 530 540 550
APRCSVCSEP IMPEPGRDET VRVVALDKNF HMKCYKCEDC GKPLSIEADD
560 570 572
NGCFPLDGHV LCRKCHTARA QT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-452 Collapse assay details

Data source: Panorama

Official Gene Symbol
ZYX
Peptide Sequence
SPGAPGPLTLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
344
Peptide End
354
CPTAC ID
CPTAC-452
Peptide Molecular Mass
1,036.5917
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 11 8.1 6.4 15.2 13.1 15.4 18.8 15.4 16.7 15 25 15
y7 (1+) 6.7 3.4 1.9 12 4.6 4.4 13.7 5.7 4.8 15 25 15
y6 (1+) 15.4 16 24.9 16.7 17.7 38.2 22.7 23.9 45.6 15 25 15
sum 6.8 3.4 2.7 12 5.2 5.2 13.8 6.2 5.9 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-453 Collapse assay details

Data source: Panorama

Official Gene Symbol
ZYX
Peptide Sequence
VNPFRPGDSEPPPAPGAQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
36
Peptide End
54
CPTAC ID
CPTAC-453
Peptide Molecular Mass
1,987.9864
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 16.5 6.8 7.3 17 8.1 8.5 23.7 10.6 11.2 15 25 15
y7 (1+) 7 7 5.7 15 6 9.4 16.6 9.2 11 15 25 15
y17 (2+) 10.4 4.7 5.7 12.3 4.5 6.3 16.1 6.5 8.5 15 25 15
sum 5.8 4.5 4.5 6.5 4.3 5.1 8.7 6.2 6.8 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-1022 Collapse assay details

Data source: Panorama

Official Gene Symbol
ZYX
Peptide Modified Sequence
S[+80.0]PGAPGPLTLK
Modification Type
Phospho (ST)
Protein - Site of Modification
344
Peptide - Site of Modification
1
Peptide Start
344
Peptide End
354
CPTAC ID
CPTAC-1022
Peptide Molecular Mass
1,116.5580
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 15.3 5.8 5 22 6.2 7.5 26.8 8.5 9 15 24 15
y8 (1+) 14.1 5.5 5.6 22.3 6 6.5 26.4 8.1 8.6 15 24 15
y9 (1+) 11.3 6.7 7.5 26.2 7.3 9.9 28.5 9.9 12.4 15 24 15
sum 14.7 5.6 5.3 22.2 6.1 6.6 26.6 8.3 8.5 15 24 15

Additional Resources and Comments