CMBL, CPTAC-46 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CMBL	N/A

Sequence Length (AA)	Molecular Weight (Da)
245	28048

Protein Name
Carboxymethylenebutenolidase homolog

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MANEAYPCPC	DIGHRLEYGG	LGREVQVEHI	KAYVTKSPVD	AGKAVIVIQD
60	70	80	90	100
IFGWQLPNTR	YIADMISGNG	YTTIVPDFFV	GQEPWDPSGD	WSIFPEWLKT
110	120	130	140	150
RNAQKIDREI	SAILKYLKQQ	CHAQKIGIVG	FCWGGTAVHH	LMMKYSEFRA
160	170	180	190	200
GVSVYGIVKD	SEDIYNLKNP	TLFIFAENDV	VIPLKDVSLL	TQKLKEHCKV
210	220	230	240	245
EYQIKTFSGQ	THGFVHRKRE	DCSPADKPYI	DEARRNLIEW	LNKYM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-45 Collapse assay details

Data source: Panorama

Official Gene Symbol: CMBL
Peptide Sequence: AGVSVYGIVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 150
Peptide End: 159

CPTAC ID: CPTAC-45
Peptide Molecular Mass: 991.5702
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	13.9	8	11.1	28.6	12.8	18.8	31.8	15.1	21.8	15	25	15
y6 (1+)	26	20.8	9.3	34	30	12.5	42.8	36.5	15.6	15	25	15
y5 (1+)	13.5	14	15	58.3	18.7	25.3	59.8	23.4	29.4	15	25	15
sum	11.5	7.6	9.8	33.6	12	16.3	35.5	14.2	19	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments