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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

LMNB1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LMNB1 LMN2, LMNB
Sequence Length (AA) Molecular Weight (Da)
586 66408
Protein Name
Lamin-B1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MATATPVPPR MGSRAGGPTT PLSPTRLSRL QEKEELRELN DRLAVYIDKV
60 70 80 90 100
RSLETENSAL QLQVTEREEV RGRELTGLKA LYETELADAR RALDDTARER
110 120 130 140 150
AKLQIELGKC KAEHDQLLLN YAKKESDLNG AQIKLREYEA ALNSKDAALA
160 170 180 190 200
TALGDKKSLE GDLEDLKDQI AQLEASLAAA KKQLADETLL KVDLENRCQS
210 220 230 240 250
LTEDLEFRKS MYEEEINETR RKHETRLVEV DSGRQIEYEY KLAQALHEMR
260 270 280 290 300
EQHDAQVRLY KEELEQTYHA KLENARLSSE MNTSTVNSAR EELMESRMRI
310 320 330 340 350
ESLSSQLSNL QKESRACLER IQELEDLLAK EKDNSRRMLT DKEREMAEIR
360 370 380 390 400
DQMQQQLNDY EQLLDVKLAL DMEISAYRKL LEGEEERLKL SPSPSSRVTV
410 420 430 440 450
SRASSSRSVR TTRGKRKRVD VEESEASSSV SISHSASATG NVCIEEIDVD
460 470 480 490 500
GKFIRLKNTS EQDQPMGGWE MIRKIGDTSV SYKYTSRYVL KAGQTVTIWA
510 520 530 540 550
ANAGVTASPP TDLIWKNQNS WGTGEDVKVI LKNSQGEEVA QRSTVFKTTI
560 570 580 586
PEEEEEEEEA AGVVVEEELF HQQGTPRASN RSCAIM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5900 Collapse assay details

Data source: Panorama

Official Gene Symbol
LMNB1
Peptide Modified Sequence
AGGPTT[+79.966331]PLSPTR
Modification Type
Phospho (ST)
Protein - Site of Modification
21
Peptide - Site of Modification
6
Peptide Start
15
Peptide End
26
CPTAC ID
CPTAC-5900
Peptide Molecular Mass
1,233.5755
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 50.2 16.9 23.3 74.2 40.2 38.4 89.6 43.6 44.9 16 25 15
b6-98 (1+) 52.8 11.2 4.3 74.6 12.6 10.6 91.4 16.9 11.4 16 26 15
b7-98 (1+) 49.5 21.5 19.4 62.5 39.5 59 79.7 45 62.1 14 26 15
b8-98 (1+) 72.9 13 12.7 112.9 14 19.4 134.4 19.1 23.2 15 26 15
y7-98 (1+) 53.7 5.8 7.9 137 6.3 12.1 147.1 8.6 14.5 16 26 15
y6 (1+) 46.4 4.8 4.9 93.5 10.7 9.9 104.4 11.7 11 16 26 15
y4 (1+) 61 19.1 11.5 65 22.4 17.5 89.1 29.4 20.9 16 26 15
y10 (2+) 64.7 7.8 6.3 149.8 10.2 11.9 163.2 12.8 13.5 16 26 15
y9 (2+) 61.1 9.7 5.5 73.4 11.7 11 95.5 15.2 12.3 15 26 15
sum 43.4 4.7 2.1 108.7 13.7 18.6 117 14.5 18.7 16 26 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-5901 Collapse assay details

Data source: Panorama

Official Gene Symbol
LMNB1
Peptide Modified Sequence
AGGPTTPLS[+79.966331]PTR
Modification Type
Phospho (ST)
Protein - Site of Modification
24
Peptide - Site of Modification
9
Peptide Start
15
Peptide End
26
CPTAC ID
CPTAC-5901
Peptide Molecular Mass
1,233.5755
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 75.1 13.2 7.6 123.9 14 7.8 144.9 19.2 10.9 16 26 15
y7-98 (1+) 60.6 10.3 11.2 79 13.1 9.3 99.6 16.7 14.6 16 26 15
y6 (1+) 36.7 6.8 4.8 58.8 8 7.4 69.3 10.5 8.8 16 26 15
y6-98 (1+) 37.3 5.6 4.2 53.2 10.3 6.4 65 11.7 7.7 16 26 15
y5 (1+) 79.4 21.6 14.5 107.6 23.9 18.5 133.7 32.2 23.5 16 26 15
y4 (1+) 59.2 18.8 11.1 78.2 24.4 11.5 98.1 30.8 16 16 26 15
y10 (2+) 64.1 13.8 10 77.8 10.9 14.4 100.8 17.6 17.5 16 26 15
y9-98 (2+) 76.6 22.3 18 77.4 22.9 18.2 108.9 32 25.6 16 26 15
b6 (1+) 64.2 8.4 6.7 91.5 15 8.3 111.8 17.2 10.7 16 26 15
sum 41.3 3.4 2.8 64.2 8.6 5.1 76.3 9.2 5.8 16 26 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-5902 Collapse assay details

Data source: Panorama

Official Gene Symbol
LMNB1
Peptide Modified Sequence
AGGPTT[+79.966331]PLS[+79.966331]PTR
Modification Type
Phospho (ST), Phospho (ST)
Protein - Site of Modification
21, 24
Peptide - Site of Modification
6, 9
Peptide Start
15
Peptide End
26
CPTAC ID
CPTAC-5902
Peptide Molecular Mass
1,313.5418
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6-98 (1+) 47.8 26 12.8 53.3 27.5 18.9 71.6 37.8 22.8 15 27 15
y9 (2+) 92.3 27.5 6.8 84.7 28.9 17 125.3 39.9 18.3 15 27 15
y10-98 (2+) 75.7 36.6 16.3 61.7 38.5 18.9 97.7 53.1 25 15 27 15
y10 (2+) 56.8 18.4 15.7 68.7 23.1 22.7 89.1 29.5 27.6 15 27 15
y6 (1+) 61.1 21.3 5.5 58.9 25.9 11.5 84.9 33.5 12.7 15 27 15
y7-98 (1+) 78 26.6 18 77.6 31.2 19.6 110 41 26.6 15 27 15
sum 39.6 9.7 6 35 16.9 13.8 52.9 19.5 15 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments