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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CASP3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CASP3 CPP32
Sequence Length (AA) Molecular Weight (Da)
277 31608
Protein Name
Caspase-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MENTENSVDS KSIKNLEPKI IHGSESMDSG ISLDNSYKMD YPEMGLCIII
60 70 80 90 100
NNKNFHKSTG MTSRSGTDVD AANLRETFRN LKYEVRNKND LTREEIVELM
110 120 130 140 150
RDVSKEDHSK RSSFVCVLLS HGEEGIIFGT NGPVDLKKIT NFFRGDRCRS
160 170 180 190 200
LTGKPKLFII QACRGTELDC GIETDSGVDD DMACHKIPVE ADFLYAYSTA
210 220 230 240 250
PGYYSWRNSK DGSWFIQSLC AMLKQYADKL EFMHILTRVN RKVATEFESF
260 270 277
SFDATFHAKK QIPCIVSMLT KELYFYH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5907 Collapse assay details

Data source: Panorama

Official Gene Symbol
CASP3
Peptide Modified Sequence
IIHGSES[+79.966331]MDSGISLDNSYK
Modification Type
Phospho (ST)
Protein - Site of Modification
27
Peptide - Site of Modification
7
Peptide Start
20
Peptide End
38
CPTAC ID
CPTAC-5907
Peptide Molecular Mass
2,131.9133
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 67.8 23.8 15 61.7 30.9 21.7 91.7 39 26.4 15 27 15
y6 (1+) 80 19.4 13.1 69.8 19.5 14.1 106.2 27.5 19.2 15 27 15
y7 (1+) 23.6 17 12.2 28.8 22.3 14.3 37.2 28 18.8 15 27 15
y8 (1+) 58.3 55.3 44.3 76.7 60.1 42.2 96.3 81.7 61.2 15 27 15
b13-98 (2+) 70 33.1 60.6 75.2 32.8 78.2 102.7 46.6 98.9 15 27 15
b13 (2+) 49.1 41.4 32.5 73 41.8 40 88 58.8 51.5 15 27 15
y4 (1+) 77.8 35.6 18.3 72.9 27.1 18.8 106.6 44.7 26.2 15 27 15
sum 25.4 11.5 9.3 24.9 13.7 11.8 35.6 17.9 15 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments