MDM2, CPTAC-5913 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MDM2	N/A

Sequence Length (AA)	Molecular Weight (Da)
491	55233

Protein Name
E3 ubiquitin-protein ligase Mdm2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MCNTNMSVPT	DGAVTTSQIP	ASEQETLVRP	KPLLLKLLKS	VGAQKDTYTM
60	70	80	90	100
KEVLFYLGQY	IMTKRLYDEK	QQHIVYCSND	LLGDLFGVPS	FSVKEHRKIY
110	120	130	140	150
TMIYRNLVVV	NQQESSDSGT	SVSENRCHLE	GGSDQKDLVQ	ELQEEKPSSS
160	170	180	190	200
HLVSRPSTSS	RRRAISETEE	NSDELSGERQ	RKRHKSDSIS	LSFDESLALC
210	220	230	240	250
VIREICCERS	SSSESTGTPS	NPDLDAGVSE	HSGDWLDQDS	VSDQFSVEFE
260	270	280	290	300
VESLDSEDYS	LSEEGQELSD	EDDEVYQVTV	YQAGESDTDS	FEEDPEISLA
310	320	330	340	350
DYWKCTSCNE	MNPPLPSHCN	RCWALRENWL	PEDKGKDKGE	ISEKAKLENS
360	370	380	390	400
TQAEEGFDVP	DCKKTIVNDS	RESCVEENDD	KITQASQSQE	SEDYSQPSTS
410	420	430	440	450
SSIIYSSQED	VKEFEREETQ	DKEESVESSL	PLNAIEPCVI	CQGRPKNGCI
460	470	480	490	491
VHGKTGHLMA	CFTCAKKLKK	RNKPCPVCRQ	PIQMIVLTYF	P

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5913 Collapse assay details

Data source: Panorama

Official Gene Symbol: MDM2
Peptide Modified Sequence: AIS[+79.966331]ETEENSDELSGER
Modification Type: Phospho (ST)
Protein - Site of Modification: 167
Peptide - Site of Modification: 3
Peptide Start: 164
Peptide End: 179

CPTAC ID: CPTAC-5913
Peptide Molecular Mass: 1,844.7313
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Sciex 5500 QTRAP
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent 425
Column Packing: Reprosil
Column Dimensions: 75um x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	64.6	32.6	29.9	75.1	33.5	29.4	99.1	46.7	41.9	15	27	15
y8 (1+)	45.7	18.1	25.1	52.8	19.7	28.3	69.8	26.8	37.8	15	27	15
y9 (1+)	44.7	17.5	16.7	46.9	25	20.2	64.8	30.5	26.2	15	27	15
y10 (1+)	26.3	19	10.2	34	26.4	14.5	43	32.5	17.7	15	27	15
y5 (1+)	38.3	21.3	22.7	58.5	32	33.2	69.9	38.4	40.2	15	27	15
y4 (1+)	32.4	16.9	12.1	33.7	29.1	15.2	46.7	33.7	19.4	15	27	15
y14-98 (2+)	36.7	27.4	12.7	30	27.3	18.8	47.4	38.7	22.7	15	27	15
b7-98 (1+)	42.8	23.4	33.3	64.8	27.6	33.1	77.7	36.2	47	15	27	15
sum	20.4	7.6	4.4	22.1	15.9	8.5	30.1	17.6	9.6	15	27	15

MDM2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5913 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

MDM2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5913 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments