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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

UTP14A

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
UTP14A SDCCAG16
Sequence Length (AA) Molecular Weight (Da)
771 87978
Protein Name
U3 small nucleolar RNA-associated protein 14 homolog A
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTANRLAESL LALSQQEELA DLPKDYLLSE SEDEGDNDGE RKHQKLLEAI
60 70 80 90 100
SSLDGKNRRK LAERSEASLK VSEFNVSSEG SGEKLVLADL LEPVKTSSSL
110 120 130 140 150
ATVKKQLSRV KSKKTVELPL NKEEIERIHR EVAFNKTAQV LSKWDPVVLK
160 170 180 190 200
NRQAEQLVFP LEKEEPAIAP IEHVLSGWKA RTPLEQEIFN LLHKNKQPVT
210 220 230 240 250
DPLLTPVEKA SLRAMSLEEA KMRRAELQRA RALQSYYEAK ARREKKIKSK
260 270 280 290 300
KYHKVVKKGK AKKALKEFEQ LRKVNPAAAL EELEKIEKAR MMERMSLKHQ
310 320 330 340 350
NSGKWAKSKA IMAKYDLEAR QAMQEQLSKN KELTQKLQVA SESEEEEGGT
360 370 380 390 400
EDVEELLVPD VVNEVQMNAD GPNPWMLRSC TSDTKEAATQ EDPEQLPELE
410 420 430 440 450
AHGVSESEGE ERPVAEEEIL LREFEERRSL RKRSELSQDA EPAGSQETKD
460 470 480 490 500
SGSQEVLSEL RVLSQKLKEN HQSRKQKASS EGTIPQVQRE EPAPEEEEPL
510 520 530 540 550
LLQRPERVQT LEELEELGKE ECFQNKELPR PVLEGQQSER TPNNRPDAPK
560 570 580 590 600
EKKKKEQMID LQNLLTTQSP SVKSLAVPTI EELEDEEERN HRQMIKEAFA
610 620 630 640 650
GDDVIRDFLK EKREAVEASK PKDVDLTLPG WGEWGGVGLK PSAKKRRRFL
660 670 680 690 700
IKAPEGPPRK DKNLPNVIIN EKRNIHAAAH QVRVLPYPFT HHWQFERTIQ
710 720 730 740 750
TPIGSTWNTQ RAFQKLTTPK VVTKPGHIIN PIKAEDVGYR SSSRSDLSVI
760 770 771
QRNPKRITTR HKKQLKKCSV D

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5932 Collapse assay details

Data source: Panorama

Official Gene Symbol
UTP14A
Peptide Sequence
DSGSQEVLSELR
Modification Type
unmodified
Protein - Site of Modification
450
Peptide - Site of Modification
N/A
Peptide Start
450
Peptide End
461
CPTAC ID
CPTAC-5932
Peptide Molecular Mass
1,318.6365
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 37.8 14 5.8 41 18.2 8.2 55.8 23 10 15 27 15
y3 (1+) 50.4 19.8 10.1 43.4 21.2 10.2 66.5 29 14.4 15 27 15
y4 (1+) 21.3 11.8 4.5 30.9 15.5 4.3 37.5 19.5 6.2 15 27 15
y5 (1+) 26 6.1 8.4 26.7 9.5 9.8 37.3 11.3 12.9 15 27 15
y6 (1+) 17.2 12.6 7.1 23.3 15.7 7.6 29 20.1 10.4 15 27 15
y7 (1+) 22 11.5 7.5 28.2 13.4 9 35.8 17.7 11.7 15 27 15
y8 (1+) 25.5 17.4 9.7 20.2 19.8 13.8 32.5 26.4 16.9 15 27 15
sum 9.5 5.6 3.2 13.9 10.2 5.6 16.8 11.6 6.4 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-5933 Collapse assay details

Data source: Panorama

Official Gene Symbol
UTP14A
Peptide Modified Sequence
DSGS[+79.966331]QEVLSELR
Modification Type
Phospho (ST)
Protein - Site of Modification
454
Peptide - Site of Modification
4
Peptide Start
450
Peptide End
461
CPTAC ID
CPTAC-5933
Peptide Molecular Mass
1,398.6028
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 14.7 9.5 7 16.6 13.4 11.5 22.2 16.4 13.5 15 27 15
y5 (1+) 22.6 10.5 4.6 22.4 12.7 12.6 31.8 16.5 13.4 15 27 15
y6 (1+) 26.7 11.7 6.7 24.9 15.9 10.1 36.5 19.7 12.1 15 27 15
y7 (1+) 33.2 9.2 3.8 33.7 17.4 8.9 47.3 19.7 9.7 15 27 15
b8-98 (1+) 32.1 19 7.6 44.5 20.4 9.6 54.9 27.9 12.2 15 27 15
b7-98 (1+) 32.4 12.5 5.7 27 16.4 9.6 42.2 20.6 11.2 15 27 15
y11 (2+) 28.3 15 8.7 34.9 14.4 14.1 44.9 20.8 16.6 15 27 15
sum 10.7 5.1 2.7 13.4 11.4 9.8 17.1 12.5 10.2 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments