XPNPEP1, CPTAC-695 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
XPNPEP1	XPNPEPL, XPNPEPL1

Sequence Length (AA)	Molecular Weight (Da)
623	69918

Protein Name
Xaa-Pro aminopeptidase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPPKVTSELL	RQLRQAMRNS	EYVTEPIQAY	IIPSGDAHQS	EYIAPCDCRR
60	70	80	90	100
AFVSGFDGSA	GTAIITEEHA	AMWTDGRYFL	QAAKQMDSNW	TLMKMGLKDT
110	120	130	140	150
PTQEDWLVSV	LPEGSRVGVD	PLIIPTDYWK	KMAKVLRSAG	HHLIPVKENL
160	170	180	190	200
VDKIWTDRPE	RPCKPLLTLG	LDYTGISWKD	KVADLRLKMA	ERNVMWFVVT
210	220	230	240	250
ALDEIAWLFN	LRGSDVEHNP	VFFSYAIIGL	ETIMLFIDGD	RIDAPSVKEH
260	270	280	290	300
LLLDLGLEAE	YRIQVHPYKS	ILSELKALCA	DLSPREKVWV	SDKASYAVSE
310	320	330	340	350
TIPKDHRCCM	PYTPICIAKA	VKNSAESEGM	RRAHIKDAVA	LCELFNWLEK
360	370	380	390	400
EVPKGGVTEI	SAADKAEEFR	RQQADFVDLS	FPTISSTGPN	GAIIHYAPVP
410	420	430	440	450
ETNRTLSLDE	VYLIDSGAQY	KDGTTDVTRT	MHFGTPTAYE	KECFTYVLKG
460	470	480	490	500
HIAVSAAVFP	TGTKGHLLDS	FARSALWDSG	LDYLHGTGHG	VGSFLNVHEG
510	520	530	540	550
PCGISYKTFS	DEPLEAGMIV	TDEPGYYEDG	AFGIRIENVV	LVVPVKTKYN
560	570	580	590	600
FNNRGSLTFE	PLTLVPIQTK	MIDVDSLTDK	ECDWLNNYHL	TCRDVIGKEL
610	620	623
QKQGRQEALE	WLIRETQPIS	KQH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-694 Collapse assay details

Data source: Panorama

Official Gene Symbol: XPNPEP1
Peptide Sequence: GSLTFEPLTLVPIQTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 555
Peptide End: 570

CPTAC ID: CPTAC-694
Peptide Molecular Mass: 1,742.9818
Species: Homo sapiens (Human)
Assay Type: Enrichment ImmunoMRM
Enrichment Method: peptide immunoaffinity
Matrix: plasma
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 Qtrap
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent NanoLC 2D
Column Packing: ReproSil-Pur C18-AQ 3 um
Column Dimensions: 100 mm x 75 um ID
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	16.3	5.7	5.6	31.1	23.8	23	35.1	24.5	23.7	15	15	15
y6 (1+)	10.1	7.8	6.8	37.2	22.2	21.9	38.5	23.5	22.9	15	15	15
y10 (1+)	17.3	6.9	8.2	31.8	20.9	21.1	36.2	22	22.6	15	15	15
sum	13.7	5.3	5.5	30.9	22.2	22	33.8	22.8	22.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

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