GNL3, CPTAC-76 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GNL3	E2IG3, NS

Sequence Length (AA)	Molecular Weight (Da)
549	61993

Protein Name
Guanine nucleotide-binding protein-like 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKRPKLKKAS	KRMTCHKRYK	IQKKVREHHR	KLRKEAKKRG	HKKPRKDPGV
60	70	80	90	100
PNSAPFKEAL	LREAELRKQR	LEELKQQQKL	DRQKELEKKR	KLETNPDIKP
110	120	130	140	150
SNVEPMEKEF	GLCKTENKAK	SGKQNSKKLY	CQELKKVIEA	SDVVLEVLDA
160	170	180	190	200
RDPLGCRCPQ	VEEAIVQSGQ	KKLVLILNKS	DLVPKENLES	WLNYLKKELP
210	220	230	240	250
TVVFRASTKP	KDKGKITKRV	KAKKNAAPFR	SEVCFGKEGL	WKLLGGFQET
260	270	280	290	300
CSKAIRVGVI	GFPNVGKSSI	INSLKQEQMC	NVGVSMGLTR	SMQVVPLDKQ
310	320	330	340	350
ITIIDSPSFI	VSPLNSSSAL	ALRSPASIEV	VKPMEAASAI	LSQADARQVV
360	370	380	390	400
LKYTVPGYRN	SLEFFTVLAQ	RRGMHQKGGI	PNVEGAAKLL	WSEWTGASLA
410	420	430	440	450
YYCHPPTSWT	PPPYFNESIV	VDMKSGFNLE	ELEKNNAQSI	RAIKGPHLAN
460	470	480	490	500
SILFQSSGLT	NGIIEEKDIH	EELPKRKERK	QEEREDDKDS	DQETVDEEVD
510	520	530	540	549
ENSSGMFAAE	ETGEALSEET	TAGEQSTRSF	ILDKIIEEDD	AYDFSTDYV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-77 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNL3
Peptide Sequence: SGFNLEELEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 425
Peptide End: 434

CPTAC ID: CPTAC-77
Peptide Molecular Mass: 1,164.5663
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	11.7	9.8	11.8	17.2	13.9	11.2	20.8	17	16.3	15	25	15
y6 (1+)	9.6	19.9	17.6	23.7	17.9	20.1	25.6	26.8	26.7	15	25	15
y5 (1+)	12.5	11.2	16.3	12.1	11.1	21.4	17.4	15.8	26.9	15	25	15
sum	6.7	9.3	9.1	11.6	9.6	13.3	13.4	13.4	16.1	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

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