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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CASP8 MCH5
Sequence Length (AA) Molecular Weight (Da)
479 55391
Protein Name
Caspase-8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDFSRNLYDI GEQLDSEDLA SLKFLSLDYI PQRKQEPIKD ALMLFQRLQE
60 70 80 90 100
KRMLEESNLS FLKELLFRIN RLDLLITYLN TRKEEMEREL QTPGRAQISA
110 120 130 140 150
YRVMLYQISE EVSRSELRSF KFLLQEEISK CKLDDDMNLL DIFIEMEKRV
160 170 180 190 200
ILGEGKLDIL KRVCAQINKS LLKIINDYEE FSKERSSSLE GSPDEFSNGE
210 220 230 240 250
ELCGVMTISD SPREQDSESQ TLDKVYQMKS KPRGYCLIIN NHNFAKAREK
260 270 280 290 300
VPKLHSIRDR NGTHLDAGAL TTTFEELHFE IKPHDDCTVE QIYEILKIYQ
310 320 330 340 350
LMDHSNMDCF ICCILSHGDK GIIYGTDGQE APIYELTSQF TGLKCPSLAG
360 370 380 390 400
KPKVFFIQAC QGDNYQKGIP VETDSEEQPY LEMDLSSPQT RYIPDEADFL
410 420 430 440 450
LGMATVNNCV SYRNPAEGTW YIQSLCQSLR ERCPRGDDIL TILTEVNYEV
460 470 479
SNKDDKKNMG KQMPQPTFTL RKKLVFPSD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-794 Collapse assay details

Data source: Panorama

Official Gene Symbol
CASP8
Peptide Sequence
FLLQEEISK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
122
Peptide End
130
CPTAC ID
CPTAC-794
Peptide Molecular Mass
1,105.6019
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 7.1 6.1 6.2 9.7 8.1 6.8 12 10.1 9.2 15 15 15
y4 (1+) 7.2 7.7 6.7 8.5 8.6 9.3 11.1 11.5 11.5 15 15 15
y5 (1+) 7.8 7.3 6.4 9.7 6.5 6.1 12.4 9.8 8.8 15 15 15
y6 (1+) 9 5.7 4.3 8.5 5.6 4.5 12.4 8 6.2 15 15 15
y7 (1+) 4.5 5.7 2.9 7.7 5 2.9 8.9 7.6 4.1 15 15 15
y8 (1+) 13.3 5.9 6.6 11.1 8.8 8.1 17.3 10.6 10.4 15 15 15
sum 3.2 2.6 2.1 5.5 2.2 2.3 6.4 3.4 3.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-795 Collapse assay details

Data source: Panorama

Official Gene Symbol
CASP8
Peptide Sequence
FLSLDYIPQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
24
Peptide End
33
CPTAC ID
CPTAC-795
Peptide Molecular Mass
1,250.6659
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 8.1 6 2.8 8.6 6.2 3.5 11.8 8.6 4.5 15 15 15
y4 (1+) 11.1 7.9 8.4 9.5 9.6 8 14.6 12.4 11.6 15 15 15
y5 (1+) 8.9 3.2 3.9 9.4 3.8 4.3 12.9 5 5.8 15 15 15
y6 (1+) 10 3.8 4.8 9.9 6 5.9 14.1 7.1 7.6 15 15 15
y7 (1+) 14 10.6 8.7 14 10.1 8.6 19.8 14.6 12.2 15 15 15
y8 (1+) 4.5 4 2.9 5.9 3.8 3.2 7.4 5.5 4.3 15 15 15
b3 (1+) 19 14.1 13.5 20.2 13.2 15.7 27.7 19.3 20.7 15 15 15
sum 3.9 2.3 1.7 3.7 2.4 2.1 5.4 3.3 2.7 15 15 15


Additional Resources and Comments