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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP2K5

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP2K5 MEK5, MKK5, PRKMK5
Sequence Length (AA) Molecular Weight (Da)
448 50112
Protein Name
Dual specificity mitogen-activated protein kinase kinase 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLWLALGPFP AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ
60 70 80 90 100
VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE
110 120 130 140 150
PLQIFPRACK PPGERNIHGL KVNTRAGPSQ HSSPAVSDSL PSNSLKKSSA
160 170 180 190 200
ELKKILANGQ MNEQDIRYRD TLGHGNGGTV YKAYHVPSGK ILAVKVILLD
210 220 230 240 250
ITLELQKQIM SELEILYKCD SSYIIGFYGA FFVENRISIC TEFMDGGSLD
260 270 280 290 300
VYRKMPEHVL GRIAVAVVKG LTYLWSLKIL HRDVKPSNML VNTRGQVKLC
310 320 330 340 350
DFGVSTQLVN SIAKTYVGTN AYMAPERISG EQYGIHSDVW SLGISFMELA
360 370 380 390 400
LGRFPYPQIQ KNQGSLMPLQ LLQCIVDEDS PVLPVGEFSE PFVHFITQCM
410 420 430 440 448
RKQPKERPAP EELMGHPFIV QFNDGNAAVV SMWVCRALEE RRSQQGPP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-818 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K5
Peptide Sequence
ILANGQMNEQDIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
155
Peptide End
167
CPTAC ID
CPTAC-818
Peptide Molecular Mass
1,500.7355
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 14.6 12 12.6 20.4 14 12.2 25.1 18.4 17.5 15 15 15
y4 (1+) 7.6 5.5 4.1 9.8 7.8 6.3 12.4 9.5 7.5 15 15 15
y5 (1+) 8.3 6.7 10.8 12 8.7 10.3 14.6 11 14.9 15 15 15
y6 (1+) 9.8 6.2 5.4 10.3 7.3 6.8 14.2 9.6 8.7 15 15 15
y7 (1+) 7.8 4 4 6.6 4.6 3.8 10.2 6.1 5.5 15 15 15
y9 (1+) 11 6.1 7 8.7 6.1 7.5 14 8.6 10.3 15 15 15
y10 (1+) 9.3 7.7 6.7 10.1 9.8 6.9 13.7 12.5 9.6 15 15 15
y11 (1+) 9.5 7 4.5 8.4 6.5 4.6 12.7 9.6 6.4 15 15 15
sum 4.2 2.3 2.1 4.6 2.4 1.7 6.2 3.3 2.7 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-819 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K5
Peptide Modified Sequence
LC[+57.0]DFGVSTQLVNSIAK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
300
Peptide - Site of Modification
2
Peptide Start
299
Peptide End
314
CPTAC ID
CPTAC-819
Peptide Molecular Mass
1,750.8924
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 46.4 12.9 12 55.1 16.2 15.7 72 20.7 19.8 15 15 15
y5 (1+) 20.4 12.9 9.8 26.3 9.3 12.9 33.3 15.9 16.2 15 15 15
y6 (1+) 33.7 23 12.3 35.8 20.6 13.1 49.2 30.9 18 15 15 15
y7 (1+) 41 24.5 12.5 37 27.3 13.3 55.2 36.7 18.3 15 15 15
y10 (1+) 19.9 9.9 6.5 17.6 9.5 6.9 26.6 13.7 9.5 15 15 15
b3 (1+) 33.2 15.1 6.3 35.3 16.5 10 48.5 22.4 11.8 15 15 15
sum 11.3 2.3 3.5 12.2 3.5 3.5 16.6 4.2 4.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments