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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

HP1BP3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
HP1BP3 N/A
Sequence Length (AA) Molecular Weight (Da)
553 61207
Protein Name
Heterochromatin protein 1-binding protein 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MATDTSQGEL VHPKALPLIV GAQLIHADKL GEKVEDSTMP IRRTVNSTRE
60 70 80 90 100
TPPKSKLAEG EEEKPEPDIS SEESVSTVEE QENETPPATS SEAEQPKGEP
110 120 130 140 150
ENEEKEENKS SEETKKDEKD QSKEKEKKVK KTIPSWATLS ASQLARAQKQ
160 170 180 190 200
TPMASSPRPK MDAILTEAIK ACFQKSGASV VAIRKYIIHK YPSLELERRG
210 220 230 240 250
YLLKQALKRE LNRGVIKQVK GKGASGSFVV VQKSRKTPQK SRNRKNRSSA
260 270 280 290 300
VDPEPQVKLE DVLPLAFTRL CEPKEASYSL IRKYVSQYYP KLRVDIRPQL
310 320 330 340 350
LKNALQRAVE RGQLEQITGK GASGTFQLKK SGEKPLLGGS LMEYAILSAI
360 370 380 390 400
AAMNEPKTCS TTALKKYVLE NHPGTNSNYQ MHLLKKTLQK CEKNGWMEQI
410 420 430 440 450
SGKGFSGTFQ LCFPYYPSPG VLFPKKEPDD SRDEDEDEDE SSEEDSEDEE
460 470 480 490 500
PPPKRRLQKK TPAKSPGKAA SVKQRGSKPA PKVSAAQRGK ARPLPKKAPP
510 520 530 540 550
KAKTPAKKTR PSSTVIKKPS GGSSKKPATS ARKEVKLPGK GKSTMKKSFR
553
VKK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-83 Collapse assay details

Data source: Panorama

Official Gene Symbol
HP1BP3
Peptide Sequence
LEDVLPLAFTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
259
Peptide End
269
CPTAC ID
CPTAC-83
Peptide Molecular Mass
1,272.7078
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 13.7 14.7 7.6 15 16.4 18.5 20.3 22 20 15 25 15
y7 (1+) 11.2 10 14 12.9 13.7 26 17.1 17 29.5 15 25 15
y6 (1+) 5.6 7.4 7.9 13.1 9.5 14.8 14.2 12 16.8 15 25 15
sum 5.2 7.8 8.2 11.9 10 12.6 13 12.7 15 15 25 15

Selectivity

Data source: Panorama

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Slope of Curve Fit for cell line
fragment_ion HCC1187 HCC1419 HCC1954 HCC70 MCF12A MDAMB175VII ZR751 ZR7530
y9 (1+) -42.16 -23.46 -11.28 -9.43 -27.34 -21.42 -24.45 -46.42
y7 (1+) -24.48 -17.82 -11.02 -9.68 -24.94 -19.85 -17.61 -35.41
y6 (1+) -31.21 -20.2 -10.31 -9.62 -23.88 -19.48 -20.29 -44.49
sum -30.55 -20.08 -10.56 -9.6 -24.53 -19.8 -20.19 -42.62

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
Fragment ion / Transition all_intra_CV all_inter_CV

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y9 (1+) 13.3 15.7 20.6
y7 (1+) 10.1 14 17.3
y6 (1+) 7.6 9.3 12
sum 7.9 9.9 12.7

Additional Resources and Comments

Resources
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Assay Details for CPTAC-82 Collapse assay details

Data source: Panorama

Official Gene Symbol
HP1BP3
Peptide Sequence
TIPSWATLSASQLAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
132
Peptide End
146
CPTAC ID
CPTAC-82
Peptide Molecular Mass
1,600.8573
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 10.5 14.8 9.5 20.5 15.9 18 23 21.7 20.4 15 25 15
y9 (1+) 15.6 14.8 6.8 16.8 13.9 10.3 22.9 20.3 12.3 15 25 15
y8 (1+) 14.8 14.7 6.5 24.1 13.9 13.7 28.3 20.2 15.2 15 25 15
sum 11.6 10.8 5 18.5 9.7 5.8 21.8 14.5 7.7 15 25 15

Selectivity

Data source: Panorama

view larger image
Slope of Curve Fit for cell line
fragment_ion HCC1187 HCC1419 HCC1954 HCC70 MCF12A MDAMB175VII ZR751 ZR7530
y8 (1+) -13.12 -8.91 -5.29 -7.21 -17.03 -12.57 -12.22 -26.24
y9 (1+) -10.86 -15.31 -7.62 -6.92 -15.73 -12.28 -14.43 -22.84
y10 (1+) -9.66 -13.93 -7.6 -6.78 -18.75 -12.44 -12.44 -43.79
sum -11.01 -12.51 -6.87 -6.96 -16.84 -12.41 -13.11 -26.71

Stability

Data source: Panorama

view larger image
Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y10 (1+) 10.6 44.5 14.9 NA 9
y9 (1+) 7.7 17.9 9.6 NA 18
y8 (1+) 15.6 13.7 17.5 NA 6.4
sum 6.1 20.2 4.7 NA 9.8
Fragment ion / Transition all_intra_CV all_inter_CV
y10 (1+) 24.7 38.6
y9 (1+) 14.2 14.6
y8 (1+) 13.4 14.2
sum 12.2 19.1

Endogenous

Data source: Panorama

view larger image
Fragment ion / Transition intra_CV inter_CV total_CV
y10 (1+) 15.7 15.7 22.2
y9 (1+) 15.2 13.6 20.4
y8 (1+) 16.3 14.8 22
sum 12.3 10.2 16

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-925 Collapse assay details

Data source: Panorama

Official Gene Symbol
HP1BP3
Peptide Modified Sequence
TIPSWATLSAS[+80.0]QLAR
Modification Type
Phospho (ST)
Protein - Site of Modification
142
Peptide - Site of Modification
11
Peptide Start
132
Peptide End
146
CPTAC ID
CPTAC-925
Peptide Molecular Mass
1,680.8236
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 60.2 11.6 14.1 65 21.2 16.6 88.6 24.2 21.8 15 24 15
y13 (2+) 43.6 11.5 11.7 33.6 21.9 11.2 55 24.7 16.2 15 24 15
y7 (1+) 41 20.4 15.2 79.1 36.1 16.4 89.1 41.5 22.4 15 24 15
sum 35.9 9.3 8.5 48.4 21.7 9.4 60.3 23.6 12.7 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments