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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP3K1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K1 MAPKKK1, MEKK, MEKK1
Sequence Length (AA) Molecular Weight (Da)
1512 164470
Protein Name
Mitogen-activated protein kinase kinase kinase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAAAGNRAS SSGFPGARAT SPEAGGGGGA LKASSAPAAA AGLLREAGSG
60 70 80 90 100
GRERADWRRR QLRKVRSVEL DQLPEQPLFL AASPPASSTS PSPEPADAAG
110 120 130 140 150
SGTGFQPVAV PPPHGAASRG GAHLTESVAA PDSGASSPAA AEPGEKRAPA
160 170 180 190 200
AEPSPAAAPA GREMENKETL KGLHKMDDRP EERMIREKLK ATCMPAWKHE
210 220 230 240 250
WLERRNRRGP VVVKPIPVKG DGSEMNHLAA ESPGEVQASA ASPASKGRRS
260 270 280 290 300
PSPGNSPSGR TVKSESPGVR RKRVSPVPFQ SGRITPPRRA PSPDGFSPYS
310 320 330 340 350
PEETNRRVNK VMRARLYLLQ QIGPNSFLIG GDSPDNKYRV FIGPQNCSCA
360 370 380 390 400
RGTFCIHLLF VMLRVFQLEP SDPMLWRKTL KNFEVESLFQ KYHSRRSSRI
410 420 430 440 450
KAPSRNTIQK FVSRMSNSHT LSSSSTSTSS SENSIKDEEE QMCPICLLGM
460 470 480 490 500
LDEESLTVCE DGCRNKLHHH CMSIWAEECR RNREPLICPL CRSKWRSHDF
510 520 530 540 550
YSHELSSPVD SPSSLRAAQQ QTVQQQPLAG SRRNQESNFN LTHYGTQQIP
560 570 580 590 600
PAYKDLAEPW IQVFGMELVG CLFSRNWNVR EMALRRLSHD VSGALLLANG
610 620 630 640 650
ESTGNSGGSS GSSPSGGATS GSSQTSISGD VVEACCSVLS MVCADPVYKV
660 670 680 690 700
YVAALKTLRA MLVYTPCHSL AERIKLQRLL QPVVDTILVK CADANSRTSQ
710 720 730 740 750
LSISTLLELC KGQAGELAVG REILKAGSIG IGGVDYVLNC ILGNQTESNN
760 770 780 790 800
WQELLGRLCL IDRLLLEFPA EFYPHIVSTD VSQAEPVEIR YKKLLSLLTF
810 820 830 840 850
ALQSIDNSHS MVGKLSRRIY LSSARMVTTV PHVFSKLLEM LSVSSSTHFT
860 870 880 890 900
RMRRRLMAIA DEVEIAEAIQ LGVEDTLDGQ QDSFLQASVP NNYLETTENS
910 920 930 940 950
SPECTVHLEK TGKGLCATKL SASSEDISER LASISVGPSS STTTTTTTTE
960 970 980 990 1000
QPKPMVQTKG RPHSQCLNSS PLSHHSQLMF PALSTPSSST PSVPAGTATD
1010 1020 1030 1040 1050
VSKHRLQGFI PCRIPSASPQ TQRKFSLQFH RNCPENKDSD KLSPVFTQSR
1060 1070 1080 1090 1100
PLPSSNIHRP KPSRPTPGNT SKQGDPSKNS MTLDLNSSSK CDDSFGCSSN
1110 1120 1130 1140 1150
SSNAVIPSDE TVFTPVEEKC RLDVNTELNS SIEDLLEASM PSSDTTVTFK
1160 1170 1180 1190 1200
SEVAVLSPEK AENDDTYKDD VNHNQKCKEK MEAEEEEALA IAMAMSASQD
1210 1220 1230 1240 1250
ALPIVPQLQV ENGEDIIIIQ QDTPETLPGH TKAKQPYRED TEWLKGQQIG
1260 1270 1280 1290 1300
LGAFSSCYQA QDVGTGTLMA VKQVTYVRNT SSEQEEVVEA LREEIRMMSH
1310 1320 1330 1340 1350
LNHPNIIRML GATCEKSNYN LFIEWMAGGS VAHLLSKYGA FKESVVINYT
1360 1370 1380 1390 1400
EQLLRGLSYL HENQIIHRDV KGANLLIDST GQRLRIADFG AAARLASKGT
1410 1420 1430 1440 1450
GAGEFQGQLL GTIAFMAPEV LRGQQYGRSC DVWSVGCAII EMACAKPPWN
1460 1470 1480 1490 1500
AEKHSNHLAL IFKIASATTA PSIPSHLSPG LRDVALRCLE LQPQDRPPSR
1510 1512
ELLKHPVFRT TW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-824 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Sequence
ASSAPAAAAGLLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
33
Peptide End
45
CPTAC ID
CPTAC-824
Peptide Molecular Mass
1,154.6408
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 12.8 9.2 7.9 13.3 8.7 6.1 18.5 12.7 10 15 15 15
y5 (1+) 12.8 4.7 5.5 12.2 6.1 6.9 17.7 7.7 8.8 15 15 15
y6 (1+) 8.9 6.7 5.1 10 5.9 4.9 13.4 8.9 7.1 15 15 15
y7 (1+) 7.7 4.4 3.2 9.7 5.9 3.2 12.4 7.4 4.5 15 15 15
y8 (1+) 5.5 2.6 5.6 7.6 4.6 5.5 9.4 5.3 7.8 15 15 15
y9 (1+) 5.8 2.4 2.1 8.3 3.2 3.6 10.1 4 4.2 15 15 15
y11 (1+) 12.4 9.2 6 11.4 9.8 8.6 16.8 13.4 10.5 15 15 15
sum 4 1.8 2.4 6.5 3.1 3.2 7.6 3.6 4 15 15 15

Additional Resources and Comments

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Comments

Assay Details for CPTAC-825 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Sequence
LLQPVVDTILVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
679
Peptide End
690
CPTAC ID
CPTAC-825
Peptide Molecular Mass
1,336.8330
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 10.5 5.9 9.1 17.2 10 10.5 20.2 11.6 13.9 15 15 15
y4 (1+) 19.3 11.2 9 22 11.9 11.4 29.3 16.3 14.5 15 15 15
y5 (1+) 13 10.9 5 13.4 11.4 9.6 18.7 15.8 10.8 15 15 15
y6 (1+) 12.8 3.9 5.5 12.6 9 7.3 18 9.8 9.1 15 15 15
y7 (1+) 6.8 9 5.7 11.2 13 6.4 13.1 15.8 8.6 15 15 15
y8 (1+) 14.9 7.7 5.7 19.8 10 6 24.8 12.6 8.3 15 15 15
y9 (1+) 8.8 4 4.1 11.9 6.5 6.4 14.8 7.6 7.6 15 15 15
b3 (1+) 17.5 7.3 11.2 14.3 12.9 15.7 22.6 14.8 19.3 15 15 15
sum 4.7 3.8 3.4 9.5 7.2 6.2 10.6 8.1 7.1 15 15 15

Additional Resources and Comments

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Assay Details for CPTAC-826 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Modified Sequence
LSAS[+80.0]SEDISER
Modification Type
Phospho (ST)
Protein - Site of Modification
923
Peptide - Site of Modification
4
Peptide Start
920
Peptide End
930
CPTAC ID
CPTAC-826
Peptide Molecular Mass
1,272.5235
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.1 10.5 8.9 13 10.9 8.8 15.9 15.1 12.5 15 15 15
y4 (1+) 9 7.3 8.2 9.6 7.2 8.7 13.2 10.3 12 15 15 15
y5 (1+) 9.9 5.8 8.6 10.2 4.2 9 14.2 7.2 12.4 15 15 15
y6 (1+) 14.7 8.5 8.3 16.8 12.8 8.2 22.3 15.4 11.7 15 15 15
y7 (1+) 13.3 12.7 10.4 15.2 9.7 10.8 20.2 16 15 15 15 15
sum 5.8 3.1 4.8 7.5 3 4.3 9.5 4.3 6.4 15 15 15

Additional Resources and Comments

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Comments

Assay Details for CPTAC-827 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Modified Sequence
LSASS[+80.0]EDISER
Modification Type
Phospho (ST)
Protein - Site of Modification
924
Peptide - Site of Modification
5
Peptide Start
920
Peptide End
930
CPTAC ID
CPTAC-827
Peptide Molecular Mass
1,272.5235
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 10.9 9.3 9.4 12.4 10.1 10.3 16.5 13.7 13.9 15 15 15
y4 (1+) 13 3.8 10.3 17.3 3.7 7.8 21.6 5.3 12.9 15 15 15
y5 (1+) 9.7 8.5 8.9 14.5 8.2 9.8 17.4 11.8 13.2 15 15 15
y6 (1+) 15.2 10.5 11.5 21.5 11.2 11.7 26.3 15.4 16.4 15 15 15
sum 6.9 3.2 4.3 9.8 2.6 4.4 12 4.1 6.2 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for CPTAC-1046 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Sequence
LSASSEDISER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
920
Peptide End
930
CPTAC ID
CPTAC-1046
Peptide Molecular Mass
1,192.5572
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 7.2 5.8 4 6 5.8 4.6 9.4 8.2 6.1 15 15 15
y9 (1+) 8.2 5.5 7.6 7.4 4.2 7.2 11 6.9 10.5 15 15 15
y8 (1+) 8.2 6.1 4.5 10.6 6 5.3 13.4 8.6 7 15 15 15
y7 (1+) 10 5.6 6.1 10.5 6 6.8 14.5 8.2 9.1 15 15 15
y6 (1+) 17.1 7.2 8.4 16.6 10.1 9 23.8 12.4 12.3 15 15 15
y5 (1+) 7.7 6.8 6.4 9.9 6.1 8.2 12.5 9.1 10.4 15 15 15
y4 (1+) 8.7 2.5 3.3 10.5 4.2 4 13.6 4.9 5.2 15 15 15
y3 (1+) 10.2 10.1 7.8 14.3 9.5 7.4 17.6 13.9 10.8 15 15 15
sum 3.9 3 2.2 4.1 2.5 3 5.7 3.9 3.7 15 15 15

Additional Resources and Comments

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Assay Details for CPTAC-829 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Modified Sequence
NS[+80.0]MTLDLNSSSK
Modification Type
Phospho (ST)
Protein - Site of Modification
1080
Peptide - Site of Modification
2
Peptide Start
1079
Peptide End
1090
CPTAC ID
CPTAC-829
Peptide Molecular Mass
1,375.5691
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 25.6 9.1 10.9 21.8 12.7 15.2 33.6 15.6 18.7 15 15 15
y4 (1+) 17.7 14.3 11.2 28.3 14.4 13.2 33.4 20.3 17.3 15 15 15
y5 (1+) 15.4 14.3 8.6 20 17 12 25.2 22.2 14.8 15 15 15
y6 (1+) 12.8 8.1 7.7 12.7 9.3 7.8 18 12.3 11 15 15 15
y7 (1+) 12.5 5.2 10.7 14.2 10 9.9 18.9 11.3 14.6 15 15 15
y8 (1+) 21 11.7 7.7 17.7 11.6 9.1 27.5 16.5 11.9 15 15 15
y9 (1+) 14.3 11.9 10.2 15.2 9.5 10.3 20.9 15.2 14.5 15 15 15
sum 5.9 4.3 5.5 6.6 4.8 4.7 8.9 6.4 7.2 15 15 15

Additional Resources and Comments

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Assay Details for CPTAC-830 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Sequence
NSMTLDLNSSSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1079
Peptide End
1090
CPTAC ID
CPTAC-830
Peptide Molecular Mass
1,295.6027
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 14.8 8.8 5.6 14 7.8 7.2 20.4 11.8 9.1 15 15 15
y4 (1+) 11 6.6 4.1 11.3 7.6 4.8 15.8 10.1 6.3 15 15 15
y5 (1+) 10.7 8.3 3.1 11.5 9.9 4.7 15.7 12.9 5.6 15 15 15
y6 (1+) 6.6 6.1 6.5 6.7 8.3 5.4 9.4 10.3 8.5 15 15 15
y7 (1+) 7.5 3.9 4.2 9.3 4.6 4.7 11.9 6 6.3 15 15 15
y8 (1+) 10.4 4.8 4.6 9 4.2 5.2 13.8 6.4 6.9 15 15 15
y9 (1+) 4.5 6.4 4.9 5.4 5.6 5.3 7 8.5 7.2 15 15 15
y10 (1+) 10.8 8.2 3.9 12.6 8.4 6.1 16.6 11.7 7.2 15 15 15
b3 (1+) 12.7 7.8 7 13 6.9 6.9 18.2 10.4 9.8 15 15 15
sum 3.7 1.5 1.9 3.7 1.9 2.2 5.2 2.4 2.9 15 15 15

Additional Resources and Comments

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Assay Details for CPTAC-831 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Modified Sequence
RAPS[+80.0]PDGFSPYSPEETNR
Modification Type
Phospho (ST)
Protein - Site of Modification
292
Peptide - Site of Modification
4
Peptide Start
289
Peptide End
306
CPTAC ID
CPTAC-831
Peptide Molecular Mass
2,085.8793
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 21.3 13.7 13.7 28 13.8 13.9 35.2 19.4 19.5 15 15 15
y4 (1+) 28.6 9.3 5.6 28.2 10 7.7 40.2 13.7 9.5 15 15 15
y5 (1+) 19 11.9 10.8 22.1 11.8 12.9 29.1 16.8 16.8 15 15 15
y6 (1+) 12.6 4.6 5.8 15.6 5.6 7.2 20.1 7.2 9.2 15 15 15
y7 (1+) 11.5 8.2 9.1 15.6 7.3 8.5 19.4 11 12.5 15 15 15
y8 (1+) 17.1 5.3 9.8 17.5 6.5 7.5 24.5 8.4 12.3 15 15 15
y9 (1+) 21.1 9.5 5.3 24.6 9.6 8 32.4 13.5 9.6 15 15 15
sum 8.6 2.2 3.6 7.2 3.5 4.8 11.2 4.1 6 15 15 15

Additional Resources and Comments

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Assay Details for CPTAC-832 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K1
Peptide Sequence
RAPSPDGFSPYSPEETNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
289
Peptide End
306
CPTAC ID
CPTAC-832
Peptide Molecular Mass
2,005.9130
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 8.6 9 14.1 9.7 9.4 14.1 13 13 19.9 15 15 15
y4 (1+) 9.3 9.5 4.9 13 9.7 7.5 16 13.6 9 15 15 15
y5 (1+) 12.5 8.4 7.6 13.5 8.9 7.6 18.4 12.2 10.7 15 15 15
y6 (1+) 4.3 2.9 4.6 7.1 3.8 3 8.3 4.8 5.5 15 15 15
y7 (1+) 8.1 9.7 6.2 9 8.6 4.4 12.1 13 7.6 15 15 15
y8 (1+) 15.1 10.5 5.3 15.4 11.7 6.2 21.6 15.7 8.2 15 15 15
y9 (1+) 12.2 3.3 4.4 13.8 6.9 5 18.4 7.6 6.7 15 15 15
b9 (1+) 14.9 8.2 6.7 17.4 9.3 7.5 22.9 12.4 10.1 15 15 15
b8 (1+) 14.9 7.9 6.4 18.7 7.4 7.3 23.9 10.8 9.7 15 15 15
b7 (1+) 11.6 4.8 5.2 12.9 3.7 5.3 17.3 6.1 7.4 15 15 15
b6 (1+) 11.2 5.5 3.8 8.3 5.5 3.6 13.9 7.8 5.2 15 15 15
b4 (1+) 8 6.5 7.9 14.4 7.4 7.8 16.5 9.8 11.1 15 15 15
sum 3.4 1.3 2 3.9 1.8 1.9 5.2 2.2 2.8 15 15 15

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