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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP3K4

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K4 KIAA0213, MAPKKK4, MEKK4, MTK1
Sequence Length (AA) Molecular Weight (Da)
1608 181685
Protein Name
Mitogen-activated protein kinase kinase kinase 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MREAAAALVP PPAFAVTPAA AMEEPPPPPP PPPPPPEPET ESEPECCLAA
60 70 80 90 100
RQEGTLGDSA CKSPESDLED FSDETNTENL YGTSPPSTPR QMKRMSTKHQ
110 120 130 140 150
RNNVGRPASR SNLKEKMNAP NQPPHKDTGK TVENVEEYSY KQEKKIRAAL
160 170 180 190 200
RTTERDRKKN VQCSFMLDSV GGSLPKKSIP DVDLNKPYLS LGCSNAKLPV
210 220 230 240 250
SVPMPIARPA RQTSRTDCPA DRLKFFETLR LLLKLTSVSK KKDREQRGQE
260 270 280 290 300
NTSGFWLNRS NELIWLELQA WHAGRTINDQ DFFLYTARQA IPDIINEILT
310 320 330 340 350
FKVDYGSFAF VRDRAGFNGT SVEGQCKATP GTKIVGYSTH HEHLQRQRVS
360 370 380 390 400
FEQVKRIMEL LEYIEALYPS LQALQKDYEK YAAKDFQDRV QALCLWLNIT
410 420 430 440 450
KDLNQKLRIM GTVLGIKNLS DIGWPVFEIP SPRPSKGNEP EYEGDDTEGE
460 470 480 490 500
LKELESSTDE SEEEQISDPR VPEIRQPIDN SFDIQSRDCI SKKLERLESE
510 520 530 540 550
DDSLGWGAPD WSTEAGFSRH CLTSIYRPFV DKALKQMGLR KLILRLHKLM
560 570 580 590 600
DGSLQRARIA LVKNDRPVEF SEFPDPMWGS DYVQLSRTPP SSEEKCSAVS
610 620 630 640 650
WEELKAMDLP SFEPAFLVLC RVLLNVIHEC LKLRLEQRPA GEPSLLSIKQ
660 670 680 690 700
LVRECKEVLK GGLLMKQYYQ FMLQEVLEDL EKPDCNIDAF EEDLHKMLMV
710 720 730 740 750
YFDYMRSWIQ MLQQLPQASH SLKNLLEEEW NFTKEITHYI RGGEAQAGKL
760 770 780 790 800
FCDIAGMLLK STGSFLEFGL QESCAEFWTS ADDSSASDEI RRSVIEISRA
810 820 830 840 850
LKELFHEARE RASKALGFAK MLRKDLEIAA EFRLSAPVRD LLDVLKSKQY
860 870 880 890 900
VKVQIPGLEN LQMFVPDTLA EEKSIILQLL NAAAGKDCSK DSDDVLIDAY
910 920 930 940 950
LLLTKHGDRA RDSEDSWGTW EAQPVKVVPQ VETVDTLRSM QVDNLLLVVM
960 970 980 990 1000
QSAHLTIQRK AFQQSIEGLM TLCQEQTSSQ PVIAKALQQL KNDALELCNR
1010 1020 1030 1040 1050
ISNAIDRVDH MFTSEFDAEV DESESVTLQQ YYREAMIQGY NFGFEYHKEV
1060 1070 1080 1090 1100
VRLMSGEFRQ KIGDKYISFA RKWMNYVLTK CESGRGTRPR WATQGFDFLQ
1110 1120 1130 1140 1150
AIEPAFISAL PEDDFLSLQA LMNECIGHVI GKPHSPVTGL YLAIHRNSPR
1160 1170 1180 1190 1200
PMKVPRCHSD PPNPHLIIPT PEGFSTRSMP SDARSHGSPA AAAAAAAAAV
1210 1220 1230 1240 1250
AASRPSPSGG DSVLPKSISS AHDTRGSSVP ENDRLASIAA ELQFRSLSRH
1260 1270 1280 1290 1300
SSPTEERDEP AYPRGDSSGS TRRSWELRTL ISQSKDTASK LGPIEAIQKS
1310 1320 1330 1340 1350
VRLFEEKRYR EMRRKNIIGQ VCDTPKSYDN VMHVGLRKVT FKWQRGNKIG
1360 1370 1380 1390 1400
EGQYGKVYTC ISVDTGELMA MKEIRFQPND HKTIKETADE LKIFEGIKHP
1410 1420 1430 1440 1450
NLVRYFGVEL HREEMYIFME YCDEGTLEEV SRLGLQEHVI RLYSKQITIA
1460 1470 1480 1490 1500
INVLHEHGIV HRDIKGANIF LTSSGLIKLG DFGCSVKLKN NAQTMPGEVN
1510 1520 1530 1540 1550
STLGTAAYMA PEVITRAKGE GHGRAADIWS LGCVVIEMVT GKRPWHEYEH
1560 1570 1580 1590 1600
NFQIMYKVGM GHKPPIPERL SPEGKDFLSH CLESDPKMRW TASQLLDHSF
1608
VKVCTDEE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-847 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K4
Peptide Sequence
SIILQLLNAAAGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
874
Peptide End
886
CPTAC ID
CPTAC-847
Peptide Molecular Mass
1,310.7922
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 16.7 11 5.2 31.6 18.8 8.2 35.7 21.8 9.7 15 15 15
y4 (1+) 16.5 16.2 9.6 28.1 15.1 11.2 32.6 22.1 14.8 15 15 15
y5 (1+) 15.6 6.6 7.3 27 8.4 10 31.2 10.7 12.4 15 15 15
y6 (1+) 12.5 9.3 5.7 30 17.4 6 32.5 19.7 8.3 15 15 15
y7 (1+) 21 13.4 6.4 28.7 13 10 35.6 18.7 11.9 15 15 15
y8 (1+) 14.1 6.6 8.1 22.5 10 7.6 26.6 12 11.1 15 15 15
y10 (1+) 17 7.7 8.8 21 10.4 9.6 27 12.9 13 15 15 15
y11 (1+) 21.9 12.9 15 26.4 18.4 13.1 34.3 22.5 19.9 15 15 15
b3 (1+) 14.2 5.5 7.2 27 9.5 9.4 30.5 11 11.8 15 15 15
sum 11.8 3.2 3.3 22.7 7.4 5.7 25.6 8.1 6.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-848 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K4
Peptide Sequence
VDYGSFAFVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
303
Peptide End
312
CPTAC ID
CPTAC-848
Peptide Molecular Mass
1,159.5662
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.9 6.3 5.2 13.1 7.6 4.5 16.4 9.9 6.9 15 15 15
y4 (1+) 6.6 6.1 4.9 9.4 6.4 4.7 11.5 8.8 6.8 15 15 15
y5 (1+) 11.1 5.5 3.3 10.1 6.8 7.4 15 8.7 8.1 15 15 15
y6 (1+) 6.4 3.2 4.5 6.8 3.6 4.9 9.3 4.8 6.7 15 15 15
y7 (1+) 4.1 3.4 3.5 4.5 4 3.8 6.1 5.2 5.2 15 15 15
y8 (1+) 5.1 3.3 3.4 6.9 2.4 3.1 8.6 4.1 4.6 15 15 15
y9 (1+) 8.6 4.8 4.4 8.1 4.1 4.1 11.8 6.3 6 15 15 15
sum 3.6 1.2 2.4 4.6 2.1 2.6 5.8 2.4 3.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments