Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K8 COT,ESTF
Sequence Length (AA) Molecular Weight (Da)
467 52925
Protein Name
Mitogen-activated protein kinase kinase kinase 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEYMSTGSDN KEEIDLLIKH LNVSDVIDIM ENLYASEEPA VYEPSLMTMC
60 70 80 90 100
QDSNQNDERS KSLLLSGQEV PWLSSVRYGT VEDLLAFANH ISNTAKHFYG
110 120 130 140 150
QRPQESGILL NMVITPQNGR YQIDSDVLLI PWKLTYRNIG SDFIPRGAFG
160 170 180 190 200
KVYLAQDIKT KKRMACKLIP VDQFKPSDVE IQACFRHENI AELYGAVLWG
210 220 230 240 250
ETVHLFMEAG EGGSVLEKLE SCGPMREFEI IWVTKHVLKG LDFLHSKKVI
260 270 280 290 300
HHDIKPSNIV FMSTKAVLVD FGLSVQMTED VYFPKDLRGT EIYMSPEVIL
310 320 330 340 350
CRGHSTKADI YSLGATLIHM QTGTPPWVKR YPRSAYPSYL YIIHKQAPPL
360 370 380 390 400
EDIADDCSPG MRELIEASLE RNPNHRPRAA DLLKHEALNP PREDQPRCQS
410 420 430 440 450
LDSALLERKR LLSRKELELP ENIADSSCTG STEESEMLKR QRSLYIDLGA
460 467
LAGYFNLVRG PPTLEYG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



loading

Loader

Assay Details for CPTAC-858 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K8
Peptide Sequence
IEASLER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
365
Peptide End
371
CPTAC ID
CPTAC-858
Peptide Molecular Mass
816.4341
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 13.3 7.1 9.2 14.9 10.9 10.2 20 13 13.7 15 15 15
y5 (1+) 6.4 7.4 7.2 8 7.1 6.9 10.2 10.3 10 15 15 15
y6 (1+) 11.5 6.5 6.5 12 7.3 6.6 16.6 9.8 9.3 15 15 15
sum 7.7 5.3 6.6 8.2 5.2 6.4 11.2 7.4 9.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-859 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K8
Peptide Sequence
NIGSDFIPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
138
Peptide End
146
CPTAC ID
CPTAC-859
Peptide Molecular Mass
1,017.5243
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 19.9 9.8 7.7 22.1 9.8 11.2 29.7 13.9 13.6 15 15 15
y4 (1+) 4.3 5.1 4.5 9.2 4.6 5.1 10.2 6.9 6.8 15 15 15
y5 (1+) 20.4 19.7 10 18.4 14.9 12.8 27.5 24.7 16.2 15 15 15
y6 (1+) 8 11.3 7.4 9 7.9 6.9 12 13.8 10.1 15 15 15
y7 (1+) 6.4 2.6 2.8 4.7 3.9 3.6 7.9 4.7 4.6 15 15 15
sum 4.9 3.1 2.1 4.7 3.5 2.6 6.8 4.7 3.3 15 15 15

Additional Resources and Comments