MAP3K8, CPTAC-858 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAP3K8	COT, ESTF

Sequence Length (AA)	Molecular Weight (Da)
467	52925

Protein Name
Mitogen-activated protein kinase kinase kinase 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEYMSTGSDN	KEEIDLLIKH	LNVSDVIDIM	ENLYASEEPA	VYEPSLMTMC
60	70	80	90	100
QDSNQNDERS	KSLLLSGQEV	PWLSSVRYGT	VEDLLAFANH	ISNTAKHFYG
110	120	130	140	150
QRPQESGILL	NMVITPQNGR	YQIDSDVLLI	PWKLTYRNIG	SDFIPRGAFG
160	170	180	190	200
KVYLAQDIKT	KKRMACKLIP	VDQFKPSDVE	IQACFRHENI	AELYGAVLWG
210	220	230	240	250
ETVHLFMEAG	EGGSVLEKLE	SCGPMREFEI	IWVTKHVLKG	LDFLHSKKVI
260	270	280	290	300
HHDIKPSNIV	FMSTKAVLVD	FGLSVQMTED	VYFPKDLRGT	EIYMSPEVIL
310	320	330	340	350
CRGHSTKADI	YSLGATLIHM	QTGTPPWVKR	YPRSAYPSYL	YIIHKQAPPL
360	370	380	390	400
EDIADDCSPG	MRELIEASLE	RNPNHRPRAA	DLLKHEALNP	PREDQPRCQS
410	420	430	440	450
LDSALLERKR	LLSRKELELP	ENIADSSCTG	STEESEMLKR	QRSLYIDLGA
460	467
LAGYFNLVRG	PPTLEYG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-858 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K8
Peptide Sequence: IEASLER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 365
Peptide End: 371

CPTAC ID: CPTAC-858
Peptide Molecular Mass: 816.4341
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	13.3	7.1	9.2	14.9	10.9	10.2	20	13	13.7	15	15	15
y5 (1+)	6.4	7.4	7.2	8	7.1	6.9	10.2	10.3	10	15	15	15
y6 (1+)	11.5	6.5	6.5	12	7.3	6.6	16.6	9.8	9.3	15	15	15
sum	7.7	5.3	6.6	8.2	5.2	6.4	11.2	7.4	9.2	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-859 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K8
Peptide Sequence: NIGSDFIPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 138
Peptide End: 146

CPTAC ID: CPTAC-859
Peptide Molecular Mass: 1,017.5243
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend