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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K9 MLK1, PRKE1
Sequence Length (AA) Molecular Weight (Da)
1104 121895
Protein Name
Mitogen-activated protein kinase kinase kinase 9
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEPSRALLGC LASAAAAAPP GEDGAGAGAE EEEEEEEEAA AAVGPGELGC
60 70 80 90 100
DAPLPYWTAV FEYEAAGEDE LTLRLGDVVE VLSKDSQVSG DEGWWTGQLN
110 120 130 140 150
QRVGIFPSNY VTPRSAFSSR CQPGGEDPSC YPPIQLLEID FAELTLEEII
160 170 180 190 200
GIGGFGKVYR AFWIGDEVAV KAARHDPDED ISQTIENVRQ EAKLFAMLKH
210 220 230 240 250
PNIIALRGVC LKEPNLCLVM EFARGGPLNR VLSGKRIPPD ILVNWAVQIA
260 270 280 290 300
RGMNYLHDEA IVPIIHRDLK SSNILILQKV ENGDLSNKIL KITDFGLARE
310 320 330 340 350
WHRTTKMSAA GTYAWMAPEV IRASMFSKGS DVWSYGVLLW ELLTGEVPFR
360 370 380 390 400
GIDGLAVAYG VAMNKLALPI PSTCPEPFAK LMEDCWNPDP HSRPSFTNIL
410 420 430 440 450
DQLTTIEESG FFEMPKDSFH CLQDNWKHEI QEMFDQLRAK EKELRTWEEE
460 470 480 490 500
LTRAALQQKN QEELLRRREQ ELAEREIDIL ERELNIIIHQ LCQEKPRVKK
510 520 530 540 550
RKGKFRKSRL KLKDGNRISL PSDFQHKFTV QASPTMDKRK SLINSRSSPP
560 570 580 590 600
ASPTIIPRLR AIQLTPGESS KTWGRSSVVP KEEGEEEEKR APKKKGRTWG
610 620 630 640 650
PGTLGQKELA SGDEGSPQRR EKANGLSTPS ESPHFHLGLK SLVDGYKQWS
660 670 680 690 700
SSAPNLVKGP RSSPALPGFT SLMEMEDEDS EGPGSGESRL QHSPSQSYLC
710 720 730 740 750
IPFPRGEDGD GPSSDGIHEE PTPVNSATST PQLTPTNSLK RGGAHHRRCE
760 770 780 790 800
VALLGCGAVL AATGLGFDLL EAGKCQLLPL EEPEPPAREE KKRREGLFQR
810 820 830 840 850
SSRPRRSTSP PSRKLFKKEE PMLLLGDPSA SLTLLSLSSI SECNSTRSLL
860 870 880 890 900
RSDSDEIVVY EMPVSPVEAP PLSPCTHNPL VNVRVERFKR DPNQSLTPTH
910 920 930 940 950
VTLTTPSQPS SHRRTPSDGA LKPETLLASR SPSSNGLSPS PGAGMLKTPS
960 970 980 990 1000
PSRDPGEFPR LPDPNVVFPP TPRRWNTQQD STLERPKTLE FLPRPRPSAN
1010 1020 1030 1040 1050
RQRLDPWWFV SPSHARSTSP ANSSSTETPS NLDSCFASSS STVEERPGLP
1060 1070 1080 1090 1100
ALLPFQAGPL PPTERTLLDL DAEGQSQDST VPLCRAELNT HRPAPYEIQQ
1104
EFWS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-860 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K9
Peptide Modified Sequence
LQHSPSQSYLC[+57.0]IPFPR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
700
Peptide - Site of Modification
11
Peptide Start
690
Peptide End
705
CPTAC ID
CPTAC-860
Peptide Molecular Mass
1,928.9567
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 32.6 15.6 27.8 42.2 17.6 22.6 53.3 23.5 35.8 15 15 15
y4 (1+) 11.1 6.6 5.2 11.2 7.4 5 15.8 9.9 7.2 15 15 15
y6 (1+) 10.7 6.3 6 13.7 8.1 5 17.4 10.3 7.8 15 15 15
y7 (1+) 16.7 12.1 6.7 16.2 12.8 8.4 23.3 17.6 10.7 15 15 15
b8 (1+) 30.1 14.9 19.3 25 26.5 23.1 39.1 30.4 30.1 15 15 15
b4 (1+) 20.5 16.8 12 25.9 15.7 14.3 33 23 18.7 15 15 15
b3 (1+) 36.7 14.9 12.2 30.4 15.4 11.1 47.7 21.4 16.5 15 15 15
sum 8.7 3.3 2.9 8.6 4.4 3 12.2 5.5 4.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-861 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K9
Peptide Sequence
TPSDGALKPETLLASR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
915
Peptide End
930
CPTAC ID
CPTAC-861
Peptide Molecular Mass
1,654.8890
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 16.6 12.4 8.6 20 12.6 8.9 26 17.7 12.4 15 15 15
y6 (1+) 17.9 6.8 7.9 17.5 8.9 9.3 25 11.2 12.2 15 15 15
y7 (1+) 24.3 14 10.8 24.6 13.8 15 34.6 19.7 18.5 15 15 15
y8 (1+) 7.5 5.7 4.9 10.1 6.6 3.7 12.6 8.7 6.1 15 15 15
y9 (1+) 14.2 20.1 17.5 23.1 21.6 15 27.1 29.5 23 15 15 15
sum 6.2 4 2.7 7 4.7 2.5 9.4 6.2 3.7 15 15 15

Additional Resources and Comments