MAP3K9, CPTAC-860 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAP3K9	MLK1, PRKE1

Sequence Length (AA)	Molecular Weight (Da)
1104	121895

Protein Name
Mitogen-activated protein kinase kinase kinase 9

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEPSRALLGC	LASAAAAAPP	GEDGAGAGAE	EEEEEEEEAA	AAVGPGELGC
60	70	80	90	100
DAPLPYWTAV	FEYEAAGEDE	LTLRLGDVVE	VLSKDSQVSG	DEGWWTGQLN
110	120	130	140	150
QRVGIFPSNY	VTPRSAFSSR	CQPGGEDPSC	YPPIQLLEID	FAELTLEEII
160	170	180	190	200
GIGGFGKVYR	AFWIGDEVAV	KAARHDPDED	ISQTIENVRQ	EAKLFAMLKH
210	220	230	240	250
PNIIALRGVC	LKEPNLCLVM	EFARGGPLNR	VLSGKRIPPD	ILVNWAVQIA
260	270	280	290	300
RGMNYLHDEA	IVPIIHRDLK	SSNILILQKV	ENGDLSNKIL	KITDFGLARE
310	320	330	340	350
WHRTTKMSAA	GTYAWMAPEV	IRASMFSKGS	DVWSYGVLLW	ELLTGEVPFR
360	370	380	390	400
GIDGLAVAYG	VAMNKLALPI	PSTCPEPFAK	LMEDCWNPDP	HSRPSFTNIL
410	420	430	440	450
DQLTTIEESG	FFEMPKDSFH	CLQDNWKHEI	QEMFDQLRAK	EKELRTWEEE
460	470	480	490	500
LTRAALQQKN	QEELLRRREQ	ELAEREIDIL	ERELNIIIHQ	LCQEKPRVKK
510	520	530	540	550
RKGKFRKSRL	KLKDGNRISL	PSDFQHKFTV	QASPTMDKRK	SLINSRSSPP
560	570	580	590	600
ASPTIIPRLR	AIQLTPGESS	KTWGRSSVVP	KEEGEEEEKR	APKKKGRTWG
610	620	630	640	650
PGTLGQKELA	SGDEGSPQRR	EKANGLSTPS	ESPHFHLGLK	SLVDGYKQWS
660	670	680	690	700
SSAPNLVKGP	RSSPALPGFT	SLMEMEDEDS	EGPGSGESRL	QHSPSQSYLC
710	720	730	740	750
IPFPRGEDGD	GPSSDGIHEE	PTPVNSATST	PQLTPTNSLK	RGGAHHRRCE
760	770	780	790	800
VALLGCGAVL	AATGLGFDLL	EAGKCQLLPL	EEPEPPAREE	KKRREGLFQR
810	820	830	840	850
SSRPRRSTSP	PSRKLFKKEE	PMLLLGDPSA	SLTLLSLSSI	SECNSTRSLL
860	870	880	890	900
RSDSDEIVVY	EMPVSPVEAP	PLSPCTHNPL	VNVRVERFKR	DPNQSLTPTH
910	920	930	940	950
VTLTTPSQPS	SHRRTPSDGA	LKPETLLASR	SPSSNGLSPS	PGAGMLKTPS
960	970	980	990	1000
PSRDPGEFPR	LPDPNVVFPP	TPRRWNTQQD	STLERPKTLE	FLPRPRPSAN
1010	1020	1030	1040	1050
RQRLDPWWFV	SPSHARSTSP	ANSSSTETPS	NLDSCFASSS	STVEERPGLP
1060	1070	1080	1090	1100
ALLPFQAGPL	PPTERTLLDL	DAEGQSQDST	VPLCRAELNT	HRPAPYEIQQ
1104
EFWS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-860 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K9
Peptide Modified Sequence: LQHSPSQSYLC[+57.0]IPFPR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 700
Peptide - Site of Modification: 11
Peptide Start: 690
Peptide End: 705

CPTAC ID: CPTAC-860
Peptide Molecular Mass: 1,928.9567
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	32.6	15.6	27.8	42.2	17.6	22.6	53.3	23.5	35.8	15	15	15
y4 (1+)	11.1	6.6	5.2	11.2	7.4	5	15.8	9.9	7.2	15	15	15
y6 (1+)	10.7	6.3	6	13.7	8.1	5	17.4	10.3	7.8	15	15	15
y7 (1+)	16.7	12.1	6.7	16.2	12.8	8.4	23.3	17.6	10.7	15	15	15
b8 (1+)	30.1	14.9	19.3	25	26.5	23.1	39.1	30.4	30.1	15	15	15
b4 (1+)	20.5	16.8	12	25.9	15.7	14.3	33	23	18.7	15	15	15
b3 (1+)	36.7	14.9	12.2	30.4	15.4	11.1	47.7	21.4	16.5	15	15	15
sum	8.7	3.3	2.9	8.6	4.4	3	12.2	5.5	4.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-861 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K9
Peptide Sequence: TPSDGALKPETLLASR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 915
Peptide End: 930

CPTAC ID: CPTAC-861
Peptide Molecular Mass: 1,654.8890
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend