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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP4K5

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP4K5 N/A
Sequence Length (AA) Molecular Weight (Da)
846 95040
Protein Name
Mitogen-activated protein kinase kinase kinase kinase 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEAPLRPAAD ILRRNPQQDY ELVQRVGSGT YGDVYKARNV HTGELAAVKI
60 70 80 90 100
IKLEPGDDFS LIQQEIFMVK ECKHCNIVAY FGSYLSREKL WICMEYCGGG
110 120 130 140 150
SLQDIYHVTG PLSELQIAYV CRETLQGLAY LHTKGKMHRD IKGANILLTD
160 170 180 190 200
HGDVKLADFG VAAKITATIA KRKSFIGTPY WMAPEVAAVE KNGGYNQLCD
210 220 230 240 250
IWAVGITAIE LGELQPPMFD LHPMRALFLM SKSNFQPPKL KDKTKWSSTF
260 270 280 290 300
HNFVKIALTK NPKKRPTAER LLTHTFVAQP GLSRALAVEL LDKVNNPDNH
310 320 330 340 350
AHYTEADDDD FEPHAIIRHT IRSTNRNARA ERTASEINFD KLQFEPPLRK
360 370 380 390 400
ETEARDEMGL SSDPNFMLQW NPFVDGANTG KSTSKRAIPP PLPPKPRISS
410 420 430 440 450
YPEDNFPDEE KASTIKHCPD SESRAPQILR RQSSPSCGPV AETSSIGNGD
460 470 480 490 500
GISKLMSENT EGSAQAPQLP RKNDKRDFPK PAINGLPPTP KVLMGACFSK
510 520 530 540 550
VFDGCPLKIN CATSWIHPDT KDQYIIFGTE DGIYTLNLNE LHEATMEQLF
560 570 580 590 600
PRKCTWLYVI NNTLMSLSEG KTFQLYSHNL IALFEHAKKP GLAAHIQTHR
610 620 630 640 650
FPDRILPRKF ALTTKIPDTK GCHKCCIVRN PYTGHKYLCG ALQSGIVLLQ
660 670 680 690 700
WYEPMQKFML IKHFDFPLPS PLNVFEMLVI PEQEYPMVCV AISKGTESNQ
710 720 730 740 750
VVQFETINLN SASSWFTEIG AGSQQLDSIH VTQLERDTVL VCLDKFVKIV
760 770 780 790 800
NLQGKLKSSK KLASELSFDF RIESVVCLQD SVLAFWKHGM QGKSFKSDEV
810 820 830 840 846
TQEISDETRV FRLLGSDRVV VLESRPTENP TAHSNLYILA GHENSY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-868 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K5
Peptide Sequence
ISSYPEDNFPDEEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
398
Peptide End
411
CPTAC ID
CPTAC-868
Peptide Molecular Mass
1,668.7155
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 19.5 14.2 10.6 20.2 13.9 7 28.1 19.9 12.7 15 15 15
y4 (1+) 22.1 11.7 9.7 19.4 11.4 11.1 29.4 16.3 14.7 15 15 15
y5 (1+) 7.5 4.7 3.6 6.6 4.5 4.1 10 6.5 5.5 15 15 15
y6 (1+) 18.1 9.9 7.1 17.5 11.1 8.8 25.2 14.9 11.3 15 15 15
y7 (1+) 18.9 13.7 8.5 15.7 13.7 12.1 24.6 19.4 14.8 15 15 15
y8 (1+) 17 10.2 9 15.2 11.4 12.9 22.8 15.3 15.7 15 15 15
y9 (1+) 14.5 9.7 7.7 20.5 9.1 13 25.1 13.3 15.1 15 15 15
y10 (1+) 10.1 8.7 6.2 10.3 7.6 5.8 14.4 11.6 8.5 15 15 15
sum 5.3 2.5 1.8 5.8 3 1.3 7.9 3.9 2.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-869 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K5
Peptide Sequence
LLTHTFVAQPGLSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
271
Peptide End
284
CPTAC ID
CPTAC-869
Peptide Molecular Mass
1,538.8569
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 8.7 3.4 5 8.3 3.2 6.1 12 4.7 7.9 15 15 15
y5 (1+) 2.8 2.9 2.3 3.3 2.9 2.8 4.3 4.1 3.6 15 15 15
y7 (1+) 20.8 15.6 18.9 20.5 9.5 17.3 29.2 18.3 25.6 15 15 15
b7 (1+) 10.6 5.7 5.3 12.2 6.4 5.3 16.2 8.6 7.5 15 15 15
b6 (1+) 8.2 7.3 6.1 10.3 6.5 6.7 13.2 9.8 9.1 15 15 15
b5 (1+) 9.6 11.1 7.5 9.2 9 8.7 13.3 14.3 11.5 15 15 15
sum 2.2 2.2 0.9 2.3 1.6 1.5 3.2 2.7 1.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments