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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

RIPK1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RIPK1 RIP, RIP1
Sequence Length (AA) Molecular Weight (Da)
671 75931
Protein Name
Receptor-interacting serine/threonine-protein kinase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPDMSLNVI KMKSSDFLES AELDSGGFGK VSLCFHRTQG LMIMKTVYKG
60 70 80 90 100
PNCIEHNEAL LEEAKMMNRL RHSRVVKLLG VIIEEGKYSL VMEYMEKGNL
110 120 130 140 150
MHVLKAEMST PLSVKGRIIL EIIEGMCYLH GKGVIHKDLK PENILVDNDF
160 170 180 190 200
HIKIADLGLA SFKMWSKLNN EEHNELREVD GTAKKNGGTL YYMAPEHLND
210 220 230 240 250
VNAKPTEKSD VYSFAVVLWA IFANKEPYEN AICEQQLIMC IKSGNRPDVD
260 270 280 290 300
DITEYCPREI ISLMKLCWEA NPEARPTFPG IEEKFRPFYL SQLEESVEED
310 320 330 340 350
VKSLKKEYSN ENAVVKRMQS LQLDCVAVPS SRSNSATEQP GSLHSSQGLG
360 370 380 390 400
MGPVEESWFA PSLEHPQEEN EPSLQSKLQD EANYHLYGSR MDRQTKQQPR
410 420 430 440 450
QNVAYNREEE RRRRVSHDPF AQQRPYENFQ NTEGKGTAYS SAASHGNAVH
460 470 480 490 500
QPSGLTSQPQ VLYQNNGLYS SHGFGTRPLD PGTAGPRVWY RPIPSHMPSL
510 520 530 540 550
HNIPVPETNY LGNTPTMPFS SLPPTDESIK YTIYNSTGIQ IGAYNYMEIG
560 570 580 590 600
GTSSSLLDST NTNFKEEPAA KYQAIFDNTT SLTDKHLDPI RENLGKHWKN
610 620 630 640 650
CARKLGFTQS QIDEIDHDYE RDGLKEKVYQ MLQKWVMREG IKGATVGKLA
660 670 671
QALHQCSRID LLSSLIYVSQ N

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1053 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK1
Peptide Sequence
IADLGLASFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
154
Peptide End
163
CPTAC ID
CPTAC-1053
Peptide Molecular Mass
1,033.5808
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 4.4 3.6 3.3 4.9 4.6 4.1 6.6 5.8 5.3 15 15 15
y8 (1+) 7.3 3.1 2.1 7.5 3.4 2.9 10.5 4.6 3.6 15 15 15
y7 (1+) 6.5 3.4 3.1 6.6 4.5 3.3 9.3 5.6 4.5 15 15 15
y6 (1+) 8.3 2.8 2.5 7.9 3.5 3.5 11.5 4.5 4.3 15 15 15
y5 (1+) 7 7.2 6.6 7.8 6.4 8.3 10.5 9.6 10.6 15 15 15
y4 (1+) 12.8 4.8 2.6 10.3 5.5 4 16.4 7.3 4.8 15 15 15
y3 (1+) 6.8 3 3 6.4 4.4 4.1 9.3 5.3 5.1 15 15 15
b3 (1+) 8.5 3.9 3.5 10.5 4.5 3.7 13.5 6 5.1 15 15 15
b5 (1+) 9.5 4.5 6.1 9 5.2 9 13.1 6.9 10.9 15 15 15
sum 5.3 2 1.2 4.3 3 2.5 6.8 3.6 2.8 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-900 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK1
Peptide Modified Sequence
MQS[+80.0]LQLDC[+57.0]VAVPSSR
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
320, 325
Peptide - Site of Modification
3, 8
Peptide Start
318
Peptide End
332
CPTAC ID
CPTAC-900
Peptide Molecular Mass
1,769.7842
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 43.5 19 21.4 44.8 14 22.1 62.4 23.6 30.8 15 15 15
y4 (1+) 16.2 6.7 8.7 18.2 5.9 9.1 24.4 8.9 12.6 15 15 15
y5 (1+) 22 10.5 12.4 22.3 11.8 11.5 31.3 15.8 16.9 15 15 15
y6 (1+) 12.5 6.7 8.4 14.7 8.6 9.3 19.3 10.9 12.5 15 15 15
y7 (1+) 41.7 19.1 12.4 49 12.3 13.2 64.3 22.7 18.1 15 15 15
y8 (1+) 24.6 30.4 25 31 25 22.7 39.6 39.4 33.8 15 15 15
y9 (1+) 98.7 36.9 40.2 107.7 37.2 33.8 146.1 52.4 52.5 15 15 15
y10 (1+) 123.9 78.6 77.6 135.9 77.2 60.5 183.9 110.2 98.4 15 15 15
y11 (1+) 92.9 119.6 86.9 85.6 98.6 95.5 126.3 155 129.1 14 12 14
y12 (1+) 173.2 141.4 71.5 141.4 173.2 57.9 223.6 223.6 92 5 7 7
b12 (1+) 24.5 NA NA 173.2 200 NA 174.9 NA NA 7 6 6
b11 (1+) 173.2 120.3 153.2 173.2 129.1 132 244.9 176.5 202.2 7 9 8
b10 (1+) 141.4 119.4 127.6 157.3 143.1 141.2 211.5 186.4 190.3 11 11 12
b9 (1+) 123 68.4 37.1 144 98.8 57.2 189.4 120.2 68.2 12 10 11
b8 (1+) 103.9 52.5 39.1 90.3 52.4 41.4 137.7 74.2 56.9 15 15 15
b7 (1+) 61.1 35.7 15.7 61.4 35.4 16.4 86.6 50.3 22.7 15 15 15
b6 (1+) 38.5 32.4 25 42.4 37 23.1 57.3 49.2 34 15 15 15
b5 (1+) 24.9 11.2 20.5 25.9 16.7 20.6 35.9 20.1 29.1 15 15 15
b4 (1+) 64.1 36 40.1 78.6 32.8 53 101.4 48.7 66.5 15 15 15
b3 (1+) 68.3 20.9 22.5 88.1 23.3 16 111.5 31.3 27.6 15 15 15
sum 8.8 4.6 4.9 8.2 5.8 5.1 12 7.4 7.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-901 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK1
Peptide Modified Sequence
MQSLQLDC[+57.0]VAVPSSR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
325
Peptide - Site of Modification
8
Peptide Start
318
Peptide End
332
CPTAC ID
CPTAC-901
Peptide Molecular Mass
1,689.8178
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 10.7 6.6 9.1 11 6.5 8.2 15.3 9.3 12.2 15 15 15
y5 (1+) 19.2 15.1 8.1 18.7 17.6 10.5 26.8 23.2 13.3 15 15 15
y6 (1+) 21 8.4 9.6 18.6 9.6 8.9 28.1 12.8 13.1 15 15 15
y8 (1+) 14.2 14.8 6.3 16.1 15.9 7.9 21.5 21.7 10.1 15 15 15
y9 (1+) 15.3 10.1 7.7 17.3 11.4 6 23.1 15.2 9.8 15 15 15
y10 (1+) 12 8.6 9.4 15.4 9 10.1 19.5 12.4 13.8 15 15 15
y13 (1+) 12 13.3 15 14 11.1 15.7 18.4 17.3 21.7 15 15 15
sum 6 3.4 2.9 7.1 3.7 3 9.3 5 4.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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View Protein in Panorama
Comments

Assay Details for CPTAC-902 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK1
Peptide Sequence
YQAIFDNTTSLTDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
572
Peptide End
585
CPTAC ID
CPTAC-902
Peptide Molecular Mass
1,615.7730
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 14.4 8.5 9.3 15.6 8 8.6 21.2 11.7 12.7 15 15 15
y4 (1+) 16.9 9.7 9 18.1 9.9 8.5 24.8 13.9 12.4 15 15 15
y5 (1+) 16.5 4.7 8 16 5.9 8.7 23 7.5 11.8 15 15 15
y6 (1+) 11.8 8.9 10.1 14.8 7.2 9.3 18.9 11.4 13.7 15 15 15
y7 (1+) 12.8 12.2 11.2 12.2 13.9 12.1 17.7 18.5 16.5 15 15 15
y8 (1+) 17 7.9 7.1 21.1 11.9 7.3 27.1 14.3 10.2 15 15 15
y9 (1+) 14.5 8.6 5.4 16.2 5.8 7.1 21.7 10.4 8.9 15 15 15
y10 (1+) 14.4 5.2 5.3 15 7.3 8.2 20.8 9 9.8 15 15 15
y11 (1+) 11.5 6.8 8.5 14.3 9.8 9 18.4 11.9 12.4 15 15 15
y12 (1+) 14.5 7.8 5.6 16.7 8.3 6.6 22.1 11.4 8.7 15 15 15
b3 (1+) 8.2 6.3 5.3 8.2 6.7 5.2 11.6 9.2 7.4 15 15 15
sum 3.4 3.6 2.6 4.3 3.4 2.5 5.5 5 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments