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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TAB2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TAB2 KIAA0733, MAP3K7IP2
Sequence Length (AA) Molecular Weight (Da)
693 76494
Protein Name
TGF-beta-activated kinase 1 and MAP3K7-binding protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAQGSHQIDF QVLHDLRQKF PEVPEVVVSR CMLQNNNNLD ACCAVLSQES
60 70 80 90 100
TRYLYGEGDL NFSDDSGISG LRNHMTSLNL DLQSQNIYHH GREGSRMNGS
110 120 130 140 150
RTLTHSISDG QLQGGQSNSE LFQQEPQTAP AQVPQGFNVF GMSSSSGASN
160 170 180 190 200
SAPHLGFHLG SKGTSSLSQQ TPRFNPIMVT LAPNIQTGRN TPTSLHIHGV
210 220 230 240 250
PPPVLNSPQG NSIYIRPYIT TPGGTTRQTQ QHSGWVSQFN PMNPQQVYQP
260 270 280 290 300
SQPGPWTTCP ASNPLSHTSS QQPNQQGHQT SHVYMPISSP TTSQPPTIHS
310 320 330 340 350
SGSSQSSAHS QYNIQNISTG PRKNQIEIKL EPPQRNNSSK LRSSGPRTSS
360 370 380 390 400
TSSSVNSQTL NRNQPTVYIA ASPPNTDELM SRSQPKVYIS ANAATGDEQV
410 420 430 440 450
MRNQPTLFIS TNSGASAASR NMSGQVSMGP AFIHHHPPKS RAIGNNSATS
460 470 480 490 500
PRVVVTQPNT KYTFKITVSP NKPPAVSPGV VSPTFELTNL LNHPDHYVET
510 520 530 540 550
ENIQHLTDPT LAHVDRISET RKLSMGSDDA AYTQALLVHQ KARMERLQRE
560 570 580 590 600
LEIQKKKLDK LKSEVNEMEN NLTRRRLKRS NSISQIPSLE EMQQLRSCNR
610 620 630 640 650
QLQIDIDCLT KEIDLFQARG PHFNPSAIHN FYDNIGFVGP VPPKPKDQRS
660 670 680 690 693
IIKTPKTQDT EDDEGAQWNC TACTFLNHPA LIRCEQCEMP RHF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1054 Collapse assay details

Data source: Panorama

Official Gene Symbol
TAB2
Peptide Sequence
AIGNNSATSPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
442
Peptide End
452
CPTAC ID
CPTAC-1054
Peptide Molecular Mass
1,086.5418
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 6.2 4 4.2 7.3 5.9 5.9 9.6 7.1 7.2 15 15 15
y9 (1+) 4.2 2.4 3.4 4.1 3.7 3.4 5.9 4.4 4.8 15 15 15
y6 (1+) 6.8 3.6 2.8 8.1 3.4 3.3 10.6 5 4.3 15 15 15
sum 3.8 1.7 2.6 4.1 3.1 2.9 5.6 3.5 3.9 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-909 Collapse assay details

Data source: Panorama

Official Gene Symbol
TAB2
Peptide Sequence
TSSTSSSVNSQTLNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
348
Peptide End
362
CPTAC ID
CPTAC-909
Peptide Molecular Mass
1,567.7438
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 43.3 16.5 16 42.5 16.5 15.4 60.7 23.3 22.2 15 15 15
y4 (1+) 17.7 15 13 19 12 12.2 26 19.2 17.8 15 15 15
y6 (1+) 14.2 10.2 5.2 17.3 10.9 6 22.4 14.9 7.9 15 15 15
y7 (1+) 14.6 7 6.5 19.3 8.3 6.1 24.2 10.9 8.9 15 15 15
y8 (1+) 21.9 7.3 7.5 20.5 9.6 8.8 30 12.1 11.6 15 15 15
y9 (1+) 18.1 7.2 7.3 16.8 8.9 9.5 24.7 11.4 12 15 15 15
y10 (1+) 15.7 8 5.9 18.5 9.6 7.1 24.3 12.5 9.2 15 15 15
y11 (1+) 8.4 3.8 6 9.7 4.7 5.6 12.8 6 8.2 15 15 15
y12 (1+) 28.9 16.9 12.1 26 21.7 10.2 38.9 27.5 15.8 15 15 15
sum 4.6 2.9 3.7 6.8 3.2 4.6 8.2 4.3 5.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments