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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ATRIP

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ATRIP AGS1
Sequence Length (AA) Molecular Weight (Da)
791 85838
Protein Name
ATR-interacting protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGTSAPGSK RRSEPPAPRP GPPPGTGHPP SKRARGFSAA AAPDPDDPFG
60 70 80 90 100
AHGDFTADDL EELDTLASQA LSQCPAAARD VSSDHKVHRL LDGMSKNPSG
110 120 130 140 150
KNRETVPIKD NFELEVLQAQ YKELKEKMKV MEEEVLIKNG EIKILRDSLH
160 170 180 190 200
QTESVLEEQR RSHFLLEQEK TQALSDKEKE FSKKLQSLQS ELQFKDAEMN
210 220 230 240 250
ELRTKLQTSE RANKLAAPSV SHVSPRKNPS VVIKPEACSP QFGKTSFPTK
260 270 280 290 300
ESFSANMSLP HPCQTESGYK PLVGREDSKP HSLRGDSIKQ EEAQKSFVDS
310 320 330 340 350
WRQRSNTQGS ILINLLLKQP LIPGSSLSLC HLLSSSSESP AGTPLQPPGF
360 370 380 390 400
GSTLAGMSGL RTTGSYDGSF SLSALREAQN LAFTGLNLVA RNECSRDGDP
410 420 430 440 450
AEGGRRAFPL CQLPGAVHFL PLVQFFIGLH CQALQDLAAA KRSGAPGDSP
460 470 480 490 500
THSSCVSSGV ETNPEDSVCI LEGFSVTALS ILQHLVCHSG AVVSLLLSGV
510 520 530 540 550
GADSAAGEGN RSLVHRLSDG DMTSALRGVA DDQGQHPLLK MLLHLLAFSS
560 570 580 590 600
AATGHLQASV LTQCLKVLVK LAENTSCDFL PRFQCVFQVL PKCLSPETPL
610 620 630 640 650
PSVLLAVELL SLLADHDQLA PQLCSHSEGC LLLLLYMYIT SRPDRVALET
660 670 680 690 700
QWLQLEQEVV WLLAKLGVQS PLPPVTGSNC QCNVEVVRAL TVMLHRQWLT
710 720 730 740 750
VRRAGGPPRT DQQRRTVRCL RDTVLLLHGL SQKDKLFMMH CVEVLHQFDQ
760 770 780 790 791
VMPGVSMLIR GLPDVTDCEE AALDDLCAAE TDVEDPEVEC G

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-914 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATRIP
Peptide Modified Sequence
LAAPSVSHVS[+80.0]PR
Modification Type
Phospho (ST)
Protein - Site of Modification
224
Peptide - Site of Modification
10
Peptide Start
215
Peptide End
226
CPTAC ID
CPTAC-914
Peptide Molecular Mass
1,299.6337
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 22.9 20.3 15.6 41.5 20.9 28 47.4 29.1 32.1 15 24 15
y9 (2+) 17.7 12 10.7 28.3 13.8 15.3 33.4 18.3 18.7 15 24 15
y11 (2+) 24.4 11.8 12.5 32.9 24 23.3 41 26.7 26.4 15 24 15
sum 19.3 10.3 11.3 29.5 16.1 18.6 35.3 19.1 21.8 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Assay Details for CPTAC-3216 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATRIP
Peptide Modified Sequence
LAAPSVSHVS[+80.0]PR
Modification Type
Phospho (ST)
Protein - Site of Modification
224
Peptide - Site of Modification
10
Peptide Start
215
Peptide End
226
CPTAC ID
CPTAC-3216
Peptide Molecular Mass
1,299.6337
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9-98 (2+) 13.1 12 5.2 25.4 15 10.6 28.6 19.2 11.8 15 15 15
y9 (2+) 14.5 9 7.2 21.7 7.7 7.3 26.1 11.8 10.3 15 15 15
y6 (1+) 28.2 12.7 13.7 29.1 13.6 15.2 40.5 18.6 20.5 15 15 15
y9 (1+) 16.8 7.8 5.9 16.3 9.1 13.6 23.4 12 14.8 15 15 15
y9-98 (1+) 23.1 12.6 14.8 33.6 16.4 12.4 40.8 20.7 19.3 15 15 15
sum 10.7 6.9 5.6 19.9 6.7 6.6 22.6 9.6 8.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-915 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATRIP
Peptide Modified Sequence
LS[+80.0]DGDMTSALR
Modification Type
Phospho (ST)
Protein - Site of Modification
518
Peptide - Site of Modification
2
Peptide Start
517
Peptide End
527
CPTAC ID
CPTAC-915
Peptide Molecular Mass
1,244.5108
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 51.8 34.5 22.4 47.3 39.4 38.7 70.1 52.4 44.7 9 20 15
y6 (1+) 27.6 23 41.3 29.2 28.6 41.5 40.2 36.7 58.5 9 20 15
y8 (1+) 39.2 29.7 31.3 34.4 34.8 40.9 52.2 45.8 51.5 9 20 15
sum 34.4 25.4 20.8 26.1 24.3 23.2 43.2 35.2 31.2 9 20 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3217 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATRIP
Peptide Sequence
LSDGDMTSALR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
517
Peptide End
527
CPTAC ID
CPTAC-3217
Peptide Molecular Mass
1,164.5445
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 23.4 11.7 10.1 27.5 15 11 36.1 19 14.9 15 15 15
y8 (1+) 20.9 13.1 13.1 27.8 14.8 10.7 34.8 19.8 16.9 15 15 15
y9 (1+) 15.9 11.4 12.6 17.3 15.6 13.9 23.5 19.3 18.8 15 15 15
y4 (1+) 10.9 11.7 6.6 14.6 17 15.7 18.2 20.6 17 15 15 15
y6 (1+) 22.2 10.4 12.8 23.6 13.2 13.1 32.4 16.8 18.3 15 15 15
sum 7.7 6.6 6.5 11.6 12 10.8 13.9 13.7 12.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments