CD3EAP, CPTAC-920 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CD3EAP	ASE1, CAST, PAF49

Sequence Length (AA)	Molecular Weight (Da)
510	54986

Protein Name
DNA-directed RNA polymerase I subunit RPA34

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEEPQAGDAA	RFSCPPNFTA	KPPASESPRF	SLEALTGPDT	ELWLIQAPAD
60	70	80	90	100
FAPECFNGRH	VPLSGSQIVK	GKLAGKRHRY	RVLSSCPQAG	EATLLAPSTE
110	120	130	140	150
AGGGLTCASA	PQGTLRILEG	PQQSLSGSPL	QPIPASPPPQ	IPPGLRPRFC
160	170	180	190	200
AFGGNPPVTG	PRSALAPNLL	TSGKKKKEMQ	VTEAPVTQEA	VNGHGALEVD
210	220	230	240	250
MALGSPEMDV	RKKKKKKNQQ	LKEPEAAGPV	GTEPTVETLE	PLGVLFPSTT
260	270	280	290	300
KKRKKPKGKE	TFEPEDKTVK	QEQINTEPLE	DTVLSPTKKR	KRQKGTEGME
310	320	330	340	350
PEEGVTVESQ	PQVKVEPLEE	AIPLPPTKKR	KKEKGQMAMM	EPGTEAMEPV
360	370	380	390	400
EPEMKPLESP	GGTMAPQQPE	GAKPQAQAAL	AAPKKKTKKE	KQQDATVEPE
410	420	430	440	450
TEVVGPELPD	DLEPQAAPTS	TKKKKKKKER	GHTVTEPIQP	LEPELPGEGQ
460	470	480	490	500
PEARATPGST	KKRKKQSQES	RMPETVPQEE	MPGPPLNSES	GEEAPTGRDK
510
KRKQQQQQPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-920 Collapse assay details

Data source: Panorama

Official Gene Symbol: CD3EAP
Peptide Modified Sequence: FSC[+57.0]PPNFTAKPPASES[+80.0]PR
Modification Type: Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification: 14, 27
Peptide - Site of Modification: 3, 16
Peptide Start: 12
Peptide End: 29

CPTAC ID: CPTAC-920
Peptide Molecular Mass: 2,068.9078
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y14 (2+)	37	25.8	31.4	43.5	26.2	48	57.1	36.8	57.4	12	24	15
y15 (2+)	42.7	25.6	24.1	32.9	31.9	32.8	53.9	40.9	40.7	12	24	15
y8 (1+)	45.9	36.5	65.3	26.7	42.1	50.8	53.1	55.7	82.7	12	24	15
sum	35.3	21.9	24	25.8	25.5	25.8	43.7	33.6	35.2	12	24	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

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