NCL, CPTAC-936 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NCL	N/A

Sequence Length (AA)	Molecular Weight (Da)
710	76614

Protein Name
Nucleolin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVKLAKAGKN	QGDPKKMAPP	PKEVEEDSED	EEMSEDEEDD	SSGEEVVIPQ
60	70	80	90	100
KKGKKAAATS	AKKVVVSPTK	KVAVATPAKK	AAVTPGKKAA	ATPAKKTVTP
110	120	130	140	150
AKAVTTPGKK	GATPGKALVA	TPGKKGAAIP	AKGAKNGKNA	KKEDSDEEED
160	170	180	190	200
DDSEEDEEDD	EDEDEDEDEI	EPAAMKAAAA	APASEDEDDE	DDEDDEDDDD
210	220	230	240	250
DEEDDSEEEA	METTPAKGKK	AAKVVPVKAK	NVAEDEDEEE	DDEDEDDDDD
260	270	280	290	300
EDDEDDDDED	DEEEEEEEEE	EPVKEAPGKR	KKEMAKQKAA	PEAKKQKVEG
310	320	330	340	350
TEPTTAFNLF	VGNLNFNKSA	PELKTGISDV	FAKNDLAVVD	VRIGMTRKFG
360	370	380	390	400
YVDFESAEDL	EKALELTGLK	VFGNEIKLEK	PKGKDSKKER	DARTLLAKNL
410	420	430	440	450
PYKVTQDELK	EVFEDAAEIR	LVSKDGKSKG	IAYIEFKTEA	DAEKTFEEKQ
460	470	480	490	500
GTEIDGRSIS	LYYTGEKGQN	QDYRGGKNST	WSGESKTLVL	SNLSYSATEE
510	520	530	540	550
TLQEVFEKAT	FIKVPQNQNG	KSKGYAFIEF	ASFEDAKEAL	NSCNKREIEG
560	570	580	590	600
RAIRLELQGP	RGSPNARSQP	SKTLFVKGLS	EDTTEETLKE	SFDGSVRARI
610	620	630	640	650
VTDRETGSSK	GFGFVDFNSE	EDAKAAKEAM	EDGEIDGNKV	TLDWAKPKGE
660	670	680	690	700
GGFGGRGGGR	GGFGGRGGGR	GGRGGFGGRG	RGGFGGRGGF	RGGRGGGGDH
710
KPQGKKTKFE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-936 Collapse assay details

Data source: Panorama

Official Gene Symbol: NCL
Peptide Modified Sequence: GFGFVDFNS[+80.0]EEDAK
Modification Type: Phospho (ST)
Protein - Site of Modification: 619
Peptide - Site of Modification: 9
Peptide Start: 611
Peptide End: 624

CPTAC ID: CPTAC-936
Peptide Molecular Mass: 1,640.6396
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	28.7	9.8	5.2	21.7	18.8	6	36	21.2	7.9	15	24	15
y10-98 (1+)	39.5	8.7	7.9	29.6	20.4	8.4	49.4	22.2	11.5	15	24	15
y10 (1+)	38.5	7.9	6.6	37.4	17.2	6.4	53.7	18.9	9.2	15	24	15
sum	29.7	7.8	5.8	21.2	18.4	5.7	36.5	20	8.1	15	24	15

NCL

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-936 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NCL

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-936 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments