RAD18, CPTAC-940 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RAD18	RNF73

Sequence Length (AA)	Molecular Weight (Da)
495	56223

Protein Name
E3 ubiquitin-protein ligase RAD18

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDSLAESRWP	PGLAVMKTID	DLLRCGICFE	YFNIAMIIPQ	CSHNYCSLCI
60	70	80	90	100
RKFLSYKTQC	PTCCVTVTEP	DLKNNRILDE	LVKSLNFARN	HLLQFALESP
110	120	130	140	150
AKSPASSSSK	NLAVKVYTPV	ASRQSLKQGS	RLMDNFLIRE	MSGSTSELLI
160	170	180	190	200
KENKSKFSPQ	KEASPAAKTK	ETRSVEEIAP	DPSEAKRPEP	PSTSTLKQVT
210	220	230	240	250
KVDCPVCGVN	IPESHINKHL	DSCLSREEKK	ESLRSSVHKR	KPLPKTVYNL
260	270	280	290	300
LSDRDLKKKL	KEHGLSIQGN	KQQLIKRHQE	FVHMYNAQCD	ALHPKSAAEI
310	320	330	340	350
VREIENIEKT	RMRLEASKLN	ESVMVFTKDQ	TEKEIDEIHS	KYRKKHKSEF
360	370	380	390	400
QLLVDQARKG	YKKIAGMSQK	TVTITKEDES	TEKLSSVCMG	QEDNMTSVTN
410	420	430	440	450
HFSQSKLDSP	EELEPDREED	SSSCIDIQEV	LSSSESDSCN	SSSSDIIRDL
460	470	480	490	495
LEEEEAWEAS	HKNDLQDTEI	SPRQNRRTRA	AESAEIEPRN	KRNRN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-940 Collapse assay details

Data source: Panorama

Official Gene Symbol: RAD18
Peptide Modified Sequence: NDLQDTEIS[+80.0]PR
Modification Type: Phospho (ST)
Protein - Site of Modification: 471
Peptide - Site of Modification: 9
Peptide Start: 463
Peptide End: 473

CPTAC ID: CPTAC-940
Peptide Molecular Mass: 1,366.5766
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (2+)	21.1	11.7	18.4	22	14.7	21.6	30.5	18.8	28.4	15	24	15
y6 (1+)	17.6	17	13.8	26.9	21.5	15.7	32.1	27.4	20.9	15	24	15
y7 (1+)	14.9	8.7	11	26.7	12.5	11.4	30.6	15.2	15.8	15	24	15
sum	11.5	9.2	11	23.8	13.9	12.5	26.4	16.7	16.7	15	24	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8-98 (1+)	28	7.9	11.1	32.5	11.4	12.3	42.9	13.9	16.6	15	15	15
y4-98 (1+)	37.3	13.6	13.7	42.4	19.6	12.4	56.5	23.9	18.5	15	15	15
y4 (1+)	37.6	9.9	7	49.6	13	8.9	62.2	16.3	11.3	15	15	15
y7-98 (1+)	16.5	8.3	8.5	33.1	20	9	37	21.7	12.4	15	15	15
y7 (1+)	18	6.9	6.7	30.9	11.4	7.2	35.8	13.3	9.8	15	15	15
sum	12.9	5.5	5.7	30.4	11.8	6.3	33	13	8.5	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	35.5	28.1	58.3	37.8	27.2	54.7	51.9	39.1	79.9	12	20	15
y6 (1+)	51	21.9	21.9	29.3	23.2	37.1	58.8	31.9	43.1	12	20	15
b7 (2+)	43.3	17.3	49.1	37.8	31.2	65.9	57.5	35.7	82.2	12	20	15
sum	42.6	13.5	22.6	22.4	15	29.8	48.1	20.2	37.4	12	20	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	9.5	15.9	8.9	10.6	36.1	7.6	14.2	39.4	11.7	15	15	15
y3 (1+)	30.4	17.2	16	34.7	29	20.4	46.1	33.7	25.9	15	15	15
y9 (1+)	21.8	12	10.4	28.5	13.4	14.2	35.9	18	17.6	15	15	15
y5 (1+)	30.6	21.3	15.3	29	24.2	20.8	42.2	32.2	25.8	15	15	15
sum	10.1	11	6.3	13.1	23.8	6.8	16.5	26.2	9.3	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	24.6	9.9	15.3	43.3	11.7	24.9	49.8	15.3	29.2	15	15	15
y4 (1+)	37.4	19.3	15.4	41.5	21.3	19.5	55.9	28.7	24.8	15	15	15
y5 (1+)	41.7	29.3	20.4	63.7	19.1	20.2	76.1	35	28.7	15	15	15
y6 (1+)	29.9	14.9	10.8	58.2	54.5	42.2	65.4	56.5	43.6	15	15	15
sum	23.5	9.5	9.4	37.8	21.7	23.1	44.5	23.7	24.9	15	15	15

Search form

RAD18

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-940 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-3247 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-939 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-3246 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-3285 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments