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Overview Data source: UniProt

Official Gene Symbol Other Aliases
TMPO LAP2
Sequence Length (AA) Molecular Weight (Da)
694 75492
Protein Name
Lamina-associated polypeptide 2, isoform alpha
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR
60 70 80 90 100
PPLPAGTNSK GPPDFSSDEE REPTPVLGSG AAAAGRSRAA VGRKATKKTD
110 120 130 140 150
KPRQEDKDDL DVTELTNEDL LDQLVKYGVN PGPIVGTTRK LYEKKLLKLR
160 170 180 190 200
EQGTESRSST PLPTISSSAE NTRQNGSNDS DRYSDNEEGK KKEHKKVKST
210 220 230 240 250
RDIVPFSELG TTPSGGGFFQ GISFPEISTR PPLGSTELQA AKKVHTSKGD
260 270 280 290 300
LPREPLVATN LPGRGQLQKL ASERNLFISC KSSHDRCLEK SSSSSSQPEH
310 320 330 340 350
SAMLVSTAAS PSLIKETTTG YYKDIVENIC GREKSGIQPL CPERSHISDQ
360 370 380 390 400
SPLSSKRKAL EESESSQLIS PPLAQAIRDY VNSLLVQGGV GSLPGTSNSM
410 420 430 440 450
PPLDVENIQK RIDQSKFQET EFLSPPRKVP RLSEKSVEER DSGSFVAFQN
460 470 480 490 500
IPGSELMSSF AKTVVSHSLT TLGLEVAKQS QHDKIDASEL SFPFHESILK
510 520 530 540 550
VIEEEWQQVD RQLPSLACKY PVSSREATQI LSVPKVDDEI LGFISEATPL
560 570 580 590 600
GGIQAASTES CNQQLDLALC RAYEAAASAL QIATHTAFVA KAMQADISQA
610 620 630 640 650
AQILSSDPSR THQALGILSK TYDAASYICE AAFDEVKMAA HTMGNATVGR
660 670 680 690 694
RYLWLKDCKI NLASKNKLAS TPFKGGTLFG GEVCKVIKKR GNKH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-949 Collapse assay details

Data source: Panorama

Official Gene Symbol
TMPO
Peptide Modified Sequence
HAS[+80.0]PILPITEFSDIPR
Modification Type
Phospho (ST)
Protein - Site of Modification
3
Peptide - Site of Modification
3
Peptide Start
1
Peptide End
16
CPTAC ID
CPTAC-949
Peptide Molecular Mass
1,871.9183
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 50.2 11.8 7.4 38.5 23.5 7.5 63.3 26.3 10.5 15 24 15
y10 (2+) 47.9 11.3 7.1 51 23.9 8.4 70 26.4 11 15 24 15
y6 (1+) 51.8 8 5.9 44.9 20.2 7.9 68.6 21.7 9.9 15 24 15
sum 48 9.8 6.5 40.8 22.3 6.6 63 24.4 9.3 15 24 15

Additional Resources and Comments