TMPO, CPTAC-949 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TMPO	LAP2

Sequence Length (AA)	Molecular Weight (Da)
694	75492

Protein Name
Lamina-associated polypeptide 2, isoform alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPEFLEDPSV	LTKDKLKSEL	VANNVTLPAG	EQRKDVYVQL	YLQHLTARNR
60	70	80	90	100
PPLPAGTNSK	GPPDFSSDEE	REPTPVLGSG	AAAAGRSRAA	VGRKATKKTD
110	120	130	140	150
KPRQEDKDDL	DVTELTNEDL	LDQLVKYGVN	PGPIVGTTRK	LYEKKLLKLR
160	170	180	190	200
EQGTESRSST	PLPTISSSAE	NTRQNGSNDS	DRYSDNEEGK	KKEHKKVKST
210	220	230	240	250
RDIVPFSELG	TTPSGGGFFQ	GISFPEISTR	PPLGSTELQA	AKKVHTSKGD
260	270	280	290	300
LPREPLVATN	LPGRGQLQKL	ASERNLFISC	KSSHDRCLEK	SSSSSSQPEH
310	320	330	340	350
SAMLVSTAAS	PSLIKETTTG	YYKDIVENIC	GREKSGIQPL	CPERSHISDQ
360	370	380	390	400
SPLSSKRKAL	EESESSQLIS	PPLAQAIRDY	VNSLLVQGGV	GSLPGTSNSM
410	420	430	440	450
PPLDVENIQK	RIDQSKFQET	EFLSPPRKVP	RLSEKSVEER	DSGSFVAFQN
460	470	480	490	500
IPGSELMSSF	AKTVVSHSLT	TLGLEVAKQS	QHDKIDASEL	SFPFHESILK
510	520	530	540	550
VIEEEWQQVD	RQLPSLACKY	PVSSREATQI	LSVPKVDDEI	LGFISEATPL
560	570	580	590	600
GGIQAASTES	CNQQLDLALC	RAYEAAASAL	QIATHTAFVA	KAMQADISQA
610	620	630	640	650
AQILSSDPSR	THQALGILSK	TYDAASYICE	AAFDEVKMAA	HTMGNATVGR
660	670	680	690	694
RYLWLKDCKI	NLASKNKLAS	TPFKGGTLFG	GEVCKVIKKR	GNKH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-949 Collapse assay details

Data source: Panorama

Official Gene Symbol: TMPO
Peptide Modified Sequence: HAS[+80.0]PILPITEFSDIPR
Modification Type: Phospho (ST)
Protein - Site of Modification: 3
Peptide - Site of Modification: 3
Peptide Start: 1
Peptide End: 16

CPTAC ID: CPTAC-949
Peptide Molecular Mass: 1,871.9183
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	50.2	11.8	7.4	38.5	23.5	7.5	63.3	26.3	10.5	15	24	15
y10 (2+)	47.9	11.3	7.1	51	23.9	8.4	70	26.4	11	15	24	15
y6 (1+)	51.8	8	5.9	44.9	20.2	7.9	68.6	21.7	9.9	15	24	15
sum	48	9.8	6.5	40.8	22.3	6.6	63	24.4	9.3	15	24	15

TMPO

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-949 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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TMPO

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-949 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments