ASF1B, CPTAC-953 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ASF1B	N/A

Sequence Length (AA)	Molecular Weight (Da)
202	22434

Protein Name
Histone chaperone ASF1B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAKVSVLNVA	VLENPSPFHS	PFRFEISFEC	SEALADDLEW	KIIYVGSAES
60	70	80	90	100
EEFDQILDSV	LVGPVPAGRH	MFVFQADAPN	PSLIPETDAV	GVTVVLITCT
110	120	130	140	150
YHGQEFIRVG	YYVNNEYLNP	ELRENPPMKP	DFSQLQRNIL	ASNPRVTRFH
160	170	180	190	200
INWDNNMDRL	EAIETQDPSL	GCGLPLNCTP	IKGLGLPGCI	PGLLPENSMD
202
CI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-953 Collapse assay details

Data source: Panorama

Official Gene Symbol: ASF1B
Peptide Modified Sequence: LEAIETQDPSLGC[+57.0]GLPLNC[+57.0]T[+80.0]PIK
Modification Type: Carbamidomethyl Cysteine, Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification: 172, 178, 179
Peptide - Site of Modification: 13, 19, 20
Peptide Start: 160
Peptide End: 182

CPTAC ID: CPTAC-953
Peptide Molecular Mass: 2,605.2169
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (2+)	50.3	13.9	14.5	46.2	38.8	14.2	68.3	41.2	20.3	15	24	15
y15 (2+)	49.5	60.2	27.3	46.8	61.3	26.8	68.1	85.9	38.3	15	24	15
y8 (1+)	36.8	12	9.8	42.6	39.4	14.1	56.3	41.2	17.2	15	24	15
sum	28.8	11.5	10.9	37.3	38.4	12.2	47.1	40.1	16.4	15	24	15

ASF1B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-953 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ASF1B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-953 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments