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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ASF1B N/A
Sequence Length (AA) Molecular Weight (Da)
202 22434
Protein Name
Histone chaperone ASF1B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES
60 70 80 90 100
EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT
110 120 130 140 150
YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH
160 170 180 190 200
INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD
202
CI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-953 Collapse assay details

Data source: Panorama

Official Gene Symbol
ASF1B
Peptide Modified Sequence
LEAIETQDPSLGC[+57.0]GLPLNC[+57.0]T[+80.0]PIK
Modification Type
Carbamidomethyl Cysteine, Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification
172, 178, 179
Peptide - Site of Modification
13, 19, 20
Peptide Start
160
Peptide End
182
CPTAC ID
CPTAC-953
Peptide Molecular Mass
2,605.2169
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 50.3 13.9 14.5 46.2 38.8 14.2 68.3 41.2 20.3 15 24 15
y15 (2+) 49.5 60.2 27.3 46.8 61.3 26.8 68.1 85.9 38.3 15 24 15
y8 (1+) 36.8 12 9.8 42.6 39.4 14.1 56.3 41.2 17.2 15 24 15
sum 28.8 11.5 10.9 37.3 38.4 12.2 47.1 40.1 16.4 15 24 15

Additional Resources and Comments