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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

GIGYF2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
GIGYF2 KIAA0642, PERQ2, TNRC15
Sequence Length (AA) Molecular Weight (Da)
1299 150070
Protein Name
PERQ amino acid-rich with GYF domain-containing protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAETQTLNF GPEWLRALSS GGSITSPPLS PALPKYKLAD YRYGREEMLA
60 70 80 90 100
LFLKDNKIPS DLLDKEFLPI LQEEPLPPLA LVPFTEEEQR NFSMSVNSAA
110 120 130 140 150
VLRLTGRGGG GTVVGAPRGR SSSRGRGRGR GECGFYQRSF DEVEGVFGRG
160 170 180 190 200
GGREMHRSQS WEERGDRRFE KPGRKDVGRP NFEEGGPTSV GRKHEFIRSE
210 220 230 240 250
SENWRIFREE QNGEDEDGGW RLAGSRRDGE RWRPHSPDGP RSAGWREHME
260 270 280 290 300
RRRRFEFDFR DRDDERGYRR VRSGSGSIDD DRDSLPEWCL EDAEEEMGTF
310 320 330 340 350
DSSGAFLSLK KVQKEPIPEE QEMDFRPVDE GEECSDSEGS HNEEAKEPDK
360 370 380 390 400
TNKKEGEKTD RVGVEASEET PQTSSSSARP GTPSDHQSQE ASQFERKDEP
410 420 430 440 450
KTEQTEKAEE ETRMENSLPA KVPSRGDEMV ADVQQPLSQI PSDTASPLLI
460 470 480 490 500
LPPPVPNPSP TLRPVETPVV GAPGMGSVST EPDDEEGLKH LEQQAEKMVA
510 520 530 540 550
YLQDSALDDE RLASKLQEHR AKGVSIPLMH EAMQKWYYKD PQGEIQGPFN
560 570 580 590 600
NQEMAEWFQA GYFTMSLLVK RACDESFQPL GDIMKMWGRV PFSPGPAPPP
610 620 630 640 650
HMGELDQERL TRQQELTALY QMQHLQYQQF LIQQQYAQVL AQQQKAALSS
660 670 680 690 700
QQQQQLALLL QQFQTLKMRI SDQNIIPSVT RSVSVPDTGS IWELQPTASQ
710 720 730 740 750
PTVWEGGSVW DLPLDTTTPG PALEQLQQLE KAKAAKLEQE RREAEMRAKR
760 770 780 790 800
EEEERKRQEE LRRQQEEILR RQQEEERKRR EEEELARRKQ EEALRRQREQ
810 820 830 840 850
EIALRRQREE EERQQQEEAL RRLEERRREE EERRKQEELL RKQEEEAAKW
860 870 880 890 900
AREEEEAQRR LEENRLRMEE EAARLRHEEE ERKRKELEVQ RQKELMRQRQ
910 920 930 940 950
QQQEALRRLQ QQQQQQQLAQ MKLPSSSTWG QQSNTTACQS QATLSLAEIQ
960 970 980 990 1000
KLEEERERQL REEQRRQQRE LMKALQQQQQ QQQQKLSGWG NVSKPSGTTK
1010 1020 1030 1040 1050
SLLEIQQEEA RQMQKQQQQQ QQHQQPNRAR NNTHSNLHTS IGNSVWGSIN
1060 1070 1080 1090 1100
TGPPNQWASD LVSSIWSNAD TKNSNMGFWD DAVKEVGPRN STNKNKNNAS
1110 1120 1130 1140 1150
LSKSVGVSNR QNKKVEEEEK LLKLFQGVNK AQDGFTQWCE QMLHALNTAN
1160 1170 1180 1190 1200
NLDVPTFVSF LKEVESPYEV HDYIRAYLGD TSEAKEFAKQ FLERRAKQKA
1210 1220 1230 1240 1250
NQQRQQQQLP QQQQQQPPQQ PPQQPQQQDS VWGMNHSTLH SVFQTNQSNN
1260 1270 1280 1290 1299
QQSNFEAVQS GKKKKKQKMV RADPSLLGFS VNASSERLNM GEIETLDDY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-969 Collapse assay details

Data source: Panorama

Official Gene Symbol
GIGYF2
Peptide Modified Sequence
S[+80.0]FDEVEGVFGR
Modification Type
Phospho (ST)
Protein - Site of Modification
139
Peptide - Site of Modification
1
Peptide Start
139
Peptide End
149
CPTAC ID
CPTAC-969
Peptide Molecular Mass
1,320.5387
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5-98 (1+) 52 17.2 14 43.9 22.1 12.5 68.1 28 18.8 15 24 15
y5 (1+) 46.4 9.5 11.2 37 14.3 14.8 59.3 17.2 18.6 15 24 15
y7 (1+) 39.8 11.1 14.9 31.2 15.7 21.2 50.6 19.2 25.9 15 24 15
sum 42.5 10.4 7 30.7 15.2 11.7 52.4 18.4 13.6 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments