NAP1L1, CPTAC-98 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NAP1L1	NRP

Sequence Length (AA)	Molecular Weight (Da)
391	45374

Protein Name
Nucleosome assembly protein 1-like 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MADIDNKEQS	ELDQDLDDVE	EVEEEETGEE	TKLKARQLTV	QMMQNPQILA
60	70	80	90	100
ALQERLDGLV	ETPTGYIESL	PRVVKRRVNA	LKNLQVKCAQ	IEAKFYEEVH
110	120	130	140	150
DLERKYAVLY	QPLFDKRFEI	INAIYEPTEE	ECEWKPDEED	EISEELKEKA
160	170	180	190	200
KIEDEKKDEE	KEDPKGIPEF	WLTVFKNVDL	LSDMVQEHDE	PILKHLKDIK
210	220	230	240	250
VKFSDAGQPM	SFVLEFHFEP	NEYFTNEVLT	KTYRMRSEPD	DSDPFSFDGP
260	270	280	290	300
EIMGCTGCQI	DWKKGKNVTL	KTIKKKQKHK	GRGTVRTVTK	TVSNDSFFNF
310	320	330	340	350
FAPPEVPESG	DLDDDAEAIL	AADFEIGHFL	RERIIPRSVL	YFTGEAIEDD
360	370	380	390	391
DDDYDEEGEE	ADEEGEEEGD	EENDPDYDPK	KDQNPAECKQ	Q

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-98 Collapse assay details

Data source: Panorama

Official Gene Symbol: NAP1L1
Peptide Sequence: GIPEFWLTVFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 166
Peptide End: 176

CPTAC ID: CPTAC-98
Peptide Molecular Mass: 1,335.7227
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	14.5	10.1	6	26.6	22.7	21	30.3	24.8	21.8	15	25	15
y7 (1+)	8	11.3	6.1	29.6	23.5	21.8	30.7	26.1	22.6	15	25	15
y6 (1+)	11.5	11.6	7.2	20	20.3	20.9	23.1	23.4	22.1	15	25	15
sum	8.2	10.9	6.3	21.9	22.1	21.3	23.4	24.6	22.2	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

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