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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PALLD KIAA0992
Sequence Length (AA) Molecular Weight (Da)
1383 150564
Protein Name
Palladin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGTSSHESF YDSLSDMQEE SKNTDFFPGL SAFLSQEEIN KSLDLARRAI
60 70 80 90 100
ADSETEDFDS EKEISQIFST SPASLCEHPS HKETKLGEHA SRRPQDNRST
110 120 130 140 150
PVQPLAEKQT KSISSPVSKR KPAMSPLLTR PSYIRSLRKA EKRGAKTPST
160 170 180 190 200
NVKPKTPHQR KGGPQSQLCD KAANLIEELT SIFKAAKPRN RSPNGESSSP
210 220 230 240 250
DSGYLSPKNQ PSALLSASAS QSPMEDQGEM EREVKSPGAR HCYQDNQDLA
260 270 280 290 300
VPHNRKSHPQ PHSALHFPAA PRFIQKLRSQ EVAEGSRVYL ECRVTGNPTP
310 320 330 340 350
RVRWFCEGKE LHNTPDIQIH CEGGDLHTLI IAEAFEDDTG RYTCLATNPS
360 370 380 390 400
GSDTTSAEVF IEGASSTDSD SESLAFKSRA GAMPQAQKKT TSVSLTIGSS
410 420 430 440 450
SPKTGVTTAV IQPLSVPVQQ VHSPTSYLCR PDGTTTAYFP PVFTKELQNT
460 470 480 490 500
AVAEGQVVVL ECRVRGAPPL QVQWFRQGSE IQDSPDFRIL QKKPRSTAEP
510 520 530 540 550
EEICTLVIAE TFPEDAGIFT CSARNDYGSA TSTAQLVVTS ANTENCSYES
560 570 580 590 600
MGESNNDHFQ HFPPPPPILE TSSLELASKK PSEIQQVNNP ELGLSRAALQ
610 620 630 640 650
MQFNAAERET NGVHPSRGVN GLINGKANSN KSLPTPAVLL SPTKEPPPLL
660 670 680 690 700
AKPKLDPLKL QQLQNQIRLE QEAGARQPPP APRSAPPSPP FPPPPAFPEL
710 720 730 740 750
AACTPPASPE PMSALASRSA PAMQSSGSFN YARPKQFIAA QNLGPASGHG
760 770 780 790 800
TPASSPSSSS LPSPMSPTPR QFGRAPVPPF AQPFGAEPEA PWGSSSPSPP
810 820 830 840 850
PPPPPVFSPT AAFPVPDVFP LPPPPPPLPS PGQASHCSSP ATRFGHSQTP
860 870 880 890 900
AAFLSALLPS QPPPAAVNAL GLPKGVTPAG FPKKASRTAR IASDEEIQGT
910 920 930 940 950
KDAVIQDLER KLRFKEDLLN NGQPRLTYEE RMARRLLGAD SATVFNIQEP
960 970 980 990 1000
EEETANQEYK VSSCEQRLIS EIEYRLERSP VDESGDEVQY GDVPVENGMA
1010 1020 1030 1040 1050
PFFEMKLKHY KIFEGMPVTF TCRVAGNPKP KIYWFKDGKQ ISPKSDHYTI
1060 1070 1080 1090 1100
QRDLDGTCSL HTTASTLDDD GNYTIMAANP QGRISCTGRL MVQAVNQRGR
1110 1120 1130 1140 1150
SPRSPSGHPH VRRPRSRSRD SGDENEPIQE RFFRPHFLQA PGDLTVQEGK
1160 1170 1180 1190 1200
LCRMDCKVSG LPTPDLSWQL DGKPVRPDSA HKMLVRENGV HSLIIEPVTS
1210 1220 1230 1240 1250
RDAGIYTCIA TNRAGQNSFS LELVVAAKEA HKPPVFIEKL QNTGVADGYP
1260 1270 1280 1290 1300
VRLECRVLGV PPPQIFWKKE NESLTHSTDR VSMHQDNHGY ICLLIQGATK
1310 1320 1330 1340 1350
EDAGWYTVSA KNEAGIVSCT ARLDVYTQWH QQSQSTKPKK VRPSASRYAA
1360 1370 1380 1383
LSDQGLDIKA AFQPEANPSH LTLNTALVES EDL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-987 Collapse assay details

Data source: Panorama

Official Gene Symbol
PALLD
Peptide Modified Sequence
IAS[+80.0]DEEIQGTK
Modification Type
Phospho (ST)
Protein - Site of Modification
893
Peptide - Site of Modification
3
Peptide Start
891
Peptide End
901
CPTAC ID
CPTAC-987
Peptide Molecular Mass
1,269.5490
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9-98 (2+) 8.4 8.9 8.5 13.5 12 9.6 15.9 14.9 12.8 15 24 15
y10-98 (2+) 9.4 8.4 8.7 19.2 10.1 10.1 21.4 13.1 13.3 15 24 15
y9 (1+) 11.7 8.9 10.3 22.4 10.3 10.7 25.3 13.6 14.9 15 24 15
sum 7.8 8.1 8.3 15.3 10 9.7 17.2 12.9 12.8 15 24 15

Additional Resources and Comments