PALLD, CPTAC-987 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PALLD	KIAA0992

Sequence Length (AA)	Molecular Weight (Da)
1383	150564

Protein Name
Palladin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSGTSSHESF	YDSLSDMQEE	SKNTDFFPGL	SAFLSQEEIN	KSLDLARRAI
60	70	80	90	100
ADSETEDFDS	EKEISQIFST	SPASLCEHPS	HKETKLGEHA	SRRPQDNRST
110	120	130	140	150
PVQPLAEKQT	KSISSPVSKR	KPAMSPLLTR	PSYIRSLRKA	EKRGAKTPST
160	170	180	190	200
NVKPKTPHQR	KGGPQSQLCD	KAANLIEELT	SIFKAAKPRN	RSPNGESSSP
210	220	230	240	250
DSGYLSPKNQ	PSALLSASAS	QSPMEDQGEM	EREVKSPGAR	HCYQDNQDLA
260	270	280	290	300
VPHNRKSHPQ	PHSALHFPAA	PRFIQKLRSQ	EVAEGSRVYL	ECRVTGNPTP
310	320	330	340	350
RVRWFCEGKE	LHNTPDIQIH	CEGGDLHTLI	IAEAFEDDTG	RYTCLATNPS
360	370	380	390	400
GSDTTSAEVF	IEGASSTDSD	SESLAFKSRA	GAMPQAQKKT	TSVSLTIGSS
410	420	430	440	450
SPKTGVTTAV	IQPLSVPVQQ	VHSPTSYLCR	PDGTTTAYFP	PVFTKELQNT
460	470	480	490	500
AVAEGQVVVL	ECRVRGAPPL	QVQWFRQGSE	IQDSPDFRIL	QKKPRSTAEP
510	520	530	540	550
EEICTLVIAE	TFPEDAGIFT	CSARNDYGSA	TSTAQLVVTS	ANTENCSYES
560	570	580	590	600
MGESNNDHFQ	HFPPPPPILE	TSSLELASKK	PSEIQQVNNP	ELGLSRAALQ
610	620	630	640	650
MQFNAAERET	NGVHPSRGVN	GLINGKANSN	KSLPTPAVLL	SPTKEPPPLL
660	670	680	690	700
AKPKLDPLKL	QQLQNQIRLE	QEAGARQPPP	APRSAPPSPP	FPPPPAFPEL
710	720	730	740	750
AACTPPASPE	PMSALASRSA	PAMQSSGSFN	YARPKQFIAA	QNLGPASGHG
760	770	780	790	800
TPASSPSSSS	LPSPMSPTPR	QFGRAPVPPF	AQPFGAEPEA	PWGSSSPSPP
810	820	830	840	850
PPPPPVFSPT	AAFPVPDVFP	LPPPPPPLPS	PGQASHCSSP	ATRFGHSQTP
860	870	880	890	900
AAFLSALLPS	QPPPAAVNAL	GLPKGVTPAG	FPKKASRTAR	IASDEEIQGT
910	920	930	940	950
KDAVIQDLER	KLRFKEDLLN	NGQPRLTYEE	RMARRLLGAD	SATVFNIQEP
960	970	980	990	1000
EEETANQEYK	VSSCEQRLIS	EIEYRLERSP	VDESGDEVQY	GDVPVENGMA
1010	1020	1030	1040	1050
PFFEMKLKHY	KIFEGMPVTF	TCRVAGNPKP	KIYWFKDGKQ	ISPKSDHYTI
1060	1070	1080	1090	1100
QRDLDGTCSL	HTTASTLDDD	GNYTIMAANP	QGRISCTGRL	MVQAVNQRGR
1110	1120	1130	1140	1150
SPRSPSGHPH	VRRPRSRSRD	SGDENEPIQE	RFFRPHFLQA	PGDLTVQEGK
1160	1170	1180	1190	1200
LCRMDCKVSG	LPTPDLSWQL	DGKPVRPDSA	HKMLVRENGV	HSLIIEPVTS
1210	1220	1230	1240	1250
RDAGIYTCIA	TNRAGQNSFS	LELVVAAKEA	HKPPVFIEKL	QNTGVADGYP
1260	1270	1280	1290	1300
VRLECRVLGV	PPPQIFWKKE	NESLTHSTDR	VSMHQDNHGY	ICLLIQGATK
1310	1320	1330	1340	1350
EDAGWYTVSA	KNEAGIVSCT	ARLDVYTQWH	QQSQSTKPKK	VRPSASRYAA
1360	1370	1380	1383
LSDQGLDIKA	AFQPEANPSH	LTLNTALVES	EDL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-987 Collapse assay details

Data source: Panorama

Official Gene Symbol: PALLD
Peptide Modified Sequence: IAS[+80.0]DEEIQGTK
Modification Type: Phospho (ST)
Protein - Site of Modification: 893
Peptide - Site of Modification: 3
Peptide Start: 891
Peptide End: 901

CPTAC ID: CPTAC-987
Peptide Molecular Mass: 1,269.5490
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9-98 (2+)	8.4	8.9	8.5	13.5	12	9.6	15.9	14.9	12.8	15	24	15
y10-98 (2+)	9.4	8.4	8.7	19.2	10.1	10.1	21.4	13.1	13.3	15	24	15
y9 (1+)	11.7	8.9	10.3	22.4	10.3	10.7	25.3	13.6	14.9	15	24	15
sum	7.8	8.1	8.3	15.3	10	9.7	17.2	12.9	12.8	15	24	15

PALLD

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-987 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PALLD

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-987 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments