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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FCN3 FCNH, HAKA1
Sequence Length (AA) Molecular Weight (Da)
299 32903
Protein Name
Ficolin-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDLLWILPSL WLLLLGGPAC LKTQEHPSCP GPRELEASKV VLLPSCPGAP
60 70 80 90 100
GSPGEKGAPG PQGPPGPPGK MGPKGEPGDP VNLLRCQEGP RNCRELLSQG
110 120 130 140 150
ATLSGWYHLC LPEGRALPVF CDMDTEGGGW LVFQRRQDGS VDFFRSWSSY
160 170 180 190 200
RAGFGNQESE FWLGNENLHQ LTLQGNWELR VELEDFNGNR TFAHYATFRL
210 220 230 240 250
LGEVDHYQLA LGKFSEGTAG DSLSLHSGRP FTTYDADHDS SNSNCAVIVH
260 270 280 290 299
GAWWYASCYR SNLNGRYAVS EAAAHKYGID WASGRGVGHP YRRVRMMLR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1124 Collapse assay details

Data source: Panorama

Official Gene Symbol
FCN3
Peptide Sequence
GVGHPYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
286
Peptide End
292
CPTAC ID
non-CPTAC-1124
Peptide Molecular Mass
784.3980
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 61.1 14.4 12.3 73.4 15.5 12 95.5 21.2 17.2 9 15 15
y4 (2+) 22.7 18.1 14.2 29.7 18.2 12.6 37.4 25.7 19 13 15 15
y5 (2+) 25.9 7 9.3 33.3 14.4 8 42.2 16 12.3 14 15 15
y3 (1+) 13.5 6.7 5 18.6 7.5 4.9 23 10.1 7 15 15 15
y5 (1+) 29 8.7 5.9 34 9.8 6.6 44.7 13.1 8.9 15 15 15
sum 13.3 6.3 3.7 15.1 5.7 4 20.1 8.5 5.4 15 15 15

Additional Resources and Comments