HBA1, CPTAC-1129 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
HBA1	N/A

Sequence Length (AA)	Molecular Weight (Da)
142	15258

Protein Name
Hemoglobin subunit alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVLSPADKTN	VKAAWGKVGA	HAGEYGAEAL	ERMFLSFPTT	KTYFPHFDLS
60	70	80	90	100
HGSAQVKGHG	KKVADALTNA	VAHVDDMPNA	LSALSDLHAH	KLRVDPVNFK
110	120	130	140	142
LLSHCLLVTL	AAHLPAEFTP	AVHASLDKFL	ASVSTVLTSK	YR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-1130 Collapse assay details

Data source: Panorama

Official Gene Symbol: HBA1
Peptide Sequence: TYFPHFDLSHGSAQVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 42
Peptide End: 57

CPTAC ID: non-CPTAC-1130
Peptide Molecular Mass: 1,832.8846
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (3+)	9.7	11.8	10.1	10.7	13.4	9.9	14.4	17.9	14.1	15	15	15
y13 (3+)	11.1	12.8	7.1	14.9	11.5	8.4	18.6	17.2	11	15	15	14
y14 (3+)	9.8	6.2	8.3	17.4	8.2	8.2	20	10.3	11.7	15	15	15
y12 (2+)	16.4	11.8	7.4	19.1	9.7	8.7	25.2	15.3	11.4	15	15	14
y13 (2+)	8.1	9.6	6.7	12	10.8	7.4	14.5	14.4	10	15	15	15
sum	4.7	4.8	4.7	10.6	6.4	3.9	11.6	8	6.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1129 Collapse assay details

Data source: Panorama

Official Gene Symbol: HBA1
Peptide Sequence: VGAHAGEYGAEALER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 18
Peptide End: 32

CPTAC ID: non-CPTAC-1129
Peptide Molecular Mass: 1,528.7270
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers