Overview Data source: UniProt

Official Gene Symbol Other Aliases
HBA1 N/A
Sequence Length (AA) Molecular Weight (Da)
142 15258
Protein Name
Hemoglobin subunit alpha
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVLSPADKTN VKAAWGKVGA HAGEYGAEAL ERMFLSFPTT KTYFPHFDLS
60 70 80 90 100
HGSAQVKGHG KKVADALTNA VAHVDDMPNA LSALSDLHAH KLRVDPVNFK
110 120 130 140 142
LLSHCLLVTL AAHLPAEFTP AVHASLDKFL ASVSTVLTSK YR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1130 Collapse assay details

Data source: Panorama

Official Gene Symbol
HBA1
Peptide Sequence
TYFPHFDLSHGSAQVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
42
Peptide End
57
CPTAC ID
non-CPTAC-1130
Peptide Molecular Mass
1,832.8846
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (3+) 9.7 11.8 10.1 10.7 13.4 9.9 14.4 17.9 14.1 15 15 15
y13 (3+) 11.1 12.8 7.1 14.9 11.5 8.4 18.6 17.2 11 15 15 14
y14 (3+) 9.8 6.2 8.3 17.4 8.2 8.2 20 10.3 11.7 15 15 15
y12 (2+) 16.4 11.8 7.4 19.1 9.7 8.7 25.2 15.3 11.4 15 15 14
y13 (2+) 8.1 9.6 6.7 12 10.8 7.4 14.5 14.4 10 15 15 15
sum 4.7 4.8 4.7 10.6 6.4 3.9 11.6 8 6.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1129 Collapse assay details

Data source: Panorama

Official Gene Symbol
HBA1
Peptide Sequence
VGAHAGEYGAEALER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
18
Peptide End
32
CPTAC ID
non-CPTAC-1129
Peptide Molecular Mass
1,528.7270
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 22.5 11.2 6.4 22.9 11.3 7 32.1 15.9 9.5 15 15 15
y3 (1+) 15.6 5.3 8.6 13 8.8 9.1 20.3 10.3 12.5 15 15 15
y4 (1+) 6.4 9.2 6.6 9.1 8.7 7.4 11.1 12.7 9.9 15 15 15
y5 (1+) 17.6 11.3 8.8 17.1 9.5 8.8 24.5 14.8 12.4 15 15 15
y7 (1+) 18.8 11 7.3 21.5 10.5 6.7 28.6 15.2 9.9 15 15 15
sum 6.5 5.8 3.9 7.1 5 4.1 9.6 7.7 5.7 15 15 15

Additional Resources and Comments