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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINA4 KST, PI4
Sequence Length (AA) Molecular Weight (Da)
427 48542
Protein Name
Kallistatin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHLIDYLLLL LVGLLALSHG QLHVEHDGES CSNSSHQQIL ETGEGSPSLK
60 70 80 90 100
IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ
110 120 130 140 150
ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK
160 170 180 190 200
FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE
210 220 230 240 250
LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ
260 270 280 290 300
EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR
310 320 330 340 350
WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG
360 370 380 390 400
ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN
410 420 427
RPFLVVIFST STQSVLFLGK VVDPTKP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-672 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA4
Peptide Modified Sequence
FLN[+1.0]DTM[+16.0]AVYEAK
Modification Type
Deamidated (NQ), Oxidation (M)
Protein - Site of Modification
157, 160
Peptide - Site of Modification
3, 6
Peptide Start
155
Peptide End
166
CPTAC ID
CPTAC-672
Peptide Molecular Mass
1,417.6436
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6 3.5 4.8 5 3.2 5.4 7.8 4.7 7.2 15 15 15
y8 (1+) 8.3 5 9.1 7.1 6.2 8.5 10.9 8 12.5 15 15 15
y7 (1+) 7.4 7.9 5.1 6.8 9 6.3 10 12 8.1 15 15 15
sum 4.4 2.8 4.4 3.7 3.3 5 5.7 4.3 6.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1135 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA4
Peptide Sequence
VGSALFLSHNLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
139
Peptide End
150
CPTAC ID
non-CPTAC-1135
Peptide Molecular Mass
1,284.7190
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 5.3 3.9 8.1 10 5.5 7.3 11.3 6.7 10.9 15 15 15
y9 (2+) 12 10.3 5 11.3 9.6 6.7 16.5 14.1 8.4 15 15 15
y10 (2+) 6.2 6.7 5.5 5.4 8.3 7 8.2 10.7 8.9 15 15 15
y11 (2+) 6.9 5.9 3.3 6.1 6.9 3.6 9.2 9.1 4.9 15 15 15
y5 (1+) 7.7 4 8.2 10.9 5.2 6.8 13.3 6.6 10.7 15 15 15
sum 4.5 4.7 2.5 4.6 5.3 2.9 6.4 7.1 3.8 15 15 15

Additional Resources and Comments