SERPINA4, CPTAC-1135 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SERPINA4	KST, PI4

Sequence Length (AA)	Molecular Weight (Da)
427	48542

Protein Name
Kallistatin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MHLIDYLLLL	LVGLLALSHG	QLHVEHDGES	CSNSSHQQIL	ETGEGSPSLK
60	70	80	90	100
IAPANADFAF	RFYYLIASET	PGKNIFFSPL	SISAAYAMLS	LGACSHSRSQ
110	120	130	140	150
ILEGLGFNLT	ELSESDVHRG	FQHLLHTLNL	PGHGLETRVG	SALFLSHNLK
160	170	180	190	200
FLAKFLNDTM	AVYEAKLFHT	NFYDTVGTIQ	LINDHVKKET	RGKIVDLVSE
210	220	230	240	250
LKKDVLMVLV	NYIYFKALWE	KPFISSRTTP	KDFYVDENTT	VRVPMMLQDQ
260	270	280	290	300
EHHWYLHDRY	LPCSVLRMDY	KGDATVFFIL	PNQGKMREIE	EVLTPEMLMR
310	320	330	340	350
WNNLLRKRNF	YKKLELHLPK	FSISGSYVLD	QILPRLGFTD	LFSKWADLSG
360	370	380	390	400
ITKQQKLEAS	KSFHKATLDV	DEAGTEAAAA	TSFAIKFFSA	QTNRHILRFN
410	420	427
RPFLVVIFST	STQSVLFLGK	VVDPTKP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-672 Collapse assay details

Data source: Panorama

Official Gene Symbol: SERPINA4
Peptide Modified Sequence: FLN[+1.0]DTM[+16.0]AVYEAK
Modification Type: Deamidated (NQ), Oxidation (M)
Protein - Site of Modification: 157, 160
Peptide - Site of Modification: 3, 6
Peptide Start: 155
Peptide End: 166

CPTAC ID: CPTAC-672
Peptide Molecular Mass: 1,417.6436
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	6	3.5	4.8	5	3.2	5.4	7.8	4.7	7.2	15	15	15
y8 (1+)	8.3	5	9.1	7.1	6.2	8.5	10.9	8	12.5	15	15	15
y7 (1+)	7.4	7.9	5.1	6.8	9	6.3	10	12	8.1	15	15	15
sum	4.4	2.8	4.4	3.7	3.3	5	5.7	4.3	6.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1135 Collapse assay details

Data source: Panorama

Official Gene Symbol: SERPINA4
Peptide Sequence: VGSALFLSHNLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 139
Peptide End: 150

CPTAC ID: non-CPTAC-1135
Peptide Molecular Mass: 1,284.7190
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers