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Overview Data source: UniProt

Official Gene Symbol Other Aliases
APOL1 APOL
Sequence Length (AA) Molecular Weight (Da)
398 43974
Protein Name
Apolipoprotein L1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEGAALLRVS VLCIWMSALF LGVGVRAEEA GARVQQNVPS GTDTGDPQSK
60 70 80 90 100
PLGDWAAGTM DPESSIFIED AIKYFKEKVS TQNLLLLLTD NEAWNGFVAA
110 120 130 140 150
AELPRNEADE LRKALDNLAR QMIMKDKNWH DKGQQYRNWF LKEFPRLKSE
160 170 180 190 200
LEDNIRRLRA LADGVQKVHK GTTIANVVSG SLSISSGILT LVGMGLAPFT
210 220 230 240 250
EGGSLVLLEP GMELGITAAL TGITSSTMDY GKKWWTQAQA HDLVIKSLDK
260 270 280 290 300
LKEVREFLGE NISNFLSLAG NTYQLTRGIG KDIRALRRAR ANLQSVPHAS
310 320 330 340 350
ASRPRVTEPI SAESGEQVER VNEPSILEMS RGVKLTDVAP VSFFLVLDVV
360 370 380 390 398
YLVYESKHLH EGAKSETAEE LKKVAQELEE KLNILNNNYK ILQADQEL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2628 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOL1
Peptide Sequence
VAQELEEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
374
Peptide End
381
CPTAC ID
non-CPTAC-2628
Peptide Molecular Mass
944.4815
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 9.5 8.8 5.7 13.3 10.4 8 16.3 13.6 9.8 15 15 15
y6 (2+) 18.8 9.4 8 13.8 11.1 9.9 23.3 14.5 12.7 15 15 15
b2 (1+) 11.7 6.1 4.5 18.3 10.8 6.8 21.7 12.4 8.2 15 15 15
sum 7.3 4.4 4.2 9.7 9.3 6.4 12.1 10.3 7.7 15 15 15

Additional Resources and Comments