APOL1, CPTAC-2628 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
APOL1	APOL

Sequence Length (AA)	Molecular Weight (Da)
398	43974

Protein Name
Apolipoprotein L1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEGAALLRVS	VLCIWMSALF	LGVGVRAEEA	GARVQQNVPS	GTDTGDPQSK
60	70	80	90	100
PLGDWAAGTM	DPESSIFIED	AIKYFKEKVS	TQNLLLLLTD	NEAWNGFVAA
110	120	130	140	150
AELPRNEADE	LRKALDNLAR	QMIMKDKNWH	DKGQQYRNWF	LKEFPRLKSE
160	170	180	190	200
LEDNIRRLRA	LADGVQKVHK	GTTIANVVSG	SLSISSGILT	LVGMGLAPFT
210	220	230	240	250
EGGSLVLLEP	GMELGITAAL	TGITSSTMDY	GKKWWTQAQA	HDLVIKSLDK
260	270	280	290	300
LKEVREFLGE	NISNFLSLAG	NTYQLTRGIG	KDIRALRRAR	ANLQSVPHAS
310	320	330	340	350
ASRPRVTEPI	SAESGEQVER	VNEPSILEMS	RGVKLTDVAP	VSFFLVLDVV
360	370	380	390	398
YLVYESKHLH	EGAKSETAEE	LKKVAQELEE	KLNILNNNYK	ILQADQEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2628 Collapse assay details

Data source: Panorama

Official Gene Symbol: APOL1
Peptide Sequence: VAQELEEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 374
Peptide End: 381

CPTAC ID: non-CPTAC-2628
Peptide Molecular Mass: 944.4815
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	9.5	8.8	5.7	13.3	10.4	8	16.3	13.6	9.8	15	15	15
y6 (2+)	18.8	9.4	8	13.8	11.1	9.9	23.3	14.5	12.7	15	15	15
b2 (1+)	11.7	6.1	4.5	18.3	10.8	6.8	21.7	12.4	8.2	15	15	15
sum	7.3	4.4	4.2	9.7	9.3	6.4	12.1	10.3	7.7	15	15	15

APOL1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2628 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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APOL1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2628 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments