PROCR, CPTAC-2664 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PROCR	EPCR

Sequence Length (AA)	Molecular Weight (Da)
238	26671

Protein Name
Endothelial protein C receptor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLTTLLPILL	LSGWAFCSQD	ASDGLQRLHM	LQISYFRDPY	HVWYQGNASL
60	70	80	90	100
GGHLTHVLEG	PDTNTTIIQL	QPLQEPESWA	RTQSGLQSYL	LQFHGLVRLV
110	120	130	140	150
HQERTLAFPL	TIRCFLGCEL	PPEGSRAHVF	FEVAVNGSSF	VSFRPERALW
160	170	180	190	200
QADTQVTSGV	VTFTLQQLNA	YNRTRYELRE	FLEDTCVQYV	QKHISAENTK
210	220	230	238
GSQTSRSYTS	LVLGVLVGSF	IIAGVAVGIF	LCTGGRRC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for non-CPTAC-2664 Collapse assay details

Data source: Panorama

Official Gene Symbol: PROCR
Peptide Sequence: TLAFPLTIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 105
Peptide End: 113

CPTAC ID: non-CPTAC-2664
Peptide Molecular Mass: 1,030.6175
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	4.5	5.2	9.7	11.4	8	12.6	12.3	9.5	15.9	15	15	15
y7 (1+)	5.3	6.8	9.4	11.1	11.2	14.2	12.3	13.1	17	15	15	15
b4 (1+)	9.4	5.8	10.1	14.3	7.6	10.6	17.1	9.6	14.6	15	15	15
b3 (1+)	4.6	4.9	7.9	12	9.4	11.8	12.9	10.6	14.2	15	15	15
y5 (1+)	8.9	7.6	11.4	11.2	9.2	11.5	14.3	11.9	16.2	15	15	15
sum	3.9	5.2	8.1	10.5	8.9	11.4	11.2	10.3	14	15	15	15

PROCR

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2664 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PROCR

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2664 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments